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  4. Mouse F-box only protein 32(FBXO32) ELISA kit

Mouse F-box only protein 32(FBXO32) ELISA kit

  • 中文名稱(chēng)：
    小鼠F-box蛋白32(FBXO32)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL008498MO
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠F-box蛋白32(FBXO32)酶聯(lián)免疫試劑盒（CSB-EL008498MO）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的FBXO32含量。FBXO32是一種重要靶點(diǎn)。它屬于F-box蛋白家族成員，在肌肉萎縮等過(guò)程中發(fā)揮關(guān)鍵作用。研究顯示其參與泛素-蛋白酶體途徑，可識(shí)別并結(jié)合底物蛋白，促使其泛素化降解，目前在肌肉相關(guān)疾病等方面的研究機(jī)制探索備受關(guān)注。試劑盒檢測(cè)范圍為37.5 pg/mL-2400 pg/mL，適用于肌肉組織代謝研究、神經(jīng)退行性疾病模型構(gòu)建、細(xì)胞應(yīng)激反應(yīng)分析等科研領(lǐng)域，為探索FBXO32在蛋白質(zhì)降解通路中的調(diào)控機(jī)制提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    Fbxo32F-box only protein 32 ELISA kit; Atrogin-1 ELISA kit; Muscle atrophy F-box protein ELISA kit; MAFbx ELISA kit
  • 縮寫(xiě)：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類(lèi)型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測(cè)范圍：
    37.5 pg/mL-2400 pg/mL
  • 靈敏度：
    9.375 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Cell Biology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線(xiàn)性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse FBXO32 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 84
    Range % 80-92
    1:2 Average % 96
    Range % 92-100
    1:4 Average % 103
    Range % 99-107
    1:8 Average % 89
    Range % 84-93
  • 回收率：
    The recovery of mouse FBXO32 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 95 89-100
    EDTA plasma (n=4) 92 88-96
  • 標(biāo)準(zhǔn)曲線(xiàn)：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    2400 2.874 2.758 2.816 2.648
    1200 2.314 2.276 2.295 2.127
    600 1.731 1.703 1.717 1.549
    300 1.105 1.139 1.122 0.954
    150 0.617 0.602 0.610 0.442
    75 0.365 0.381 0.373 0.205
    37.5 0.268 0.254 0.261 0.093
    0 0.166 0.170 0.168 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1.
  • 基因功能參考文獻(xiàn)：
    1. Oligonol-mediated downregulation of Atrogin-1 and MuRF1 gene expression alleviates muscle loss and improves the impaired myotube formation, indicating that oligonol supplementation may be useful for the attenuation of myotube loss. PMID: 28930190
    2. Indoxyl sulfate enhanced the production of atrogin-1 by enhancing oxidative stress in skeletal muscle, leading to muscle atrophy. PMID: 27549031
    3. Valproic acid attenuated muscle wasting and myotube atrophy and reduced C/EBPbeta binding to atrogin1 promoter locus in the myotubes. PMID: 27122162
    4. These results have revealed the roles for atrogin-1 in the regulation of smooth muscle contractility through enhancement of myocardin ubiquitylation/degradation and its transcriptional activity. PMID: 27403897
    5. MAFbx mRNA expression was decreased in old mice relative to adult mice, whereas MuRF1 mRNA expression was less affected by ageing PMID: 26590085
    6. Suggest role for atrogin-1 up-regulation in simvastatin-induced heart mitochondria dysfunction. PMID: 25300705
    7. Atrogin1 was upregulated in cancer cachexia mice. Atrogin1 knockdown protected skeletal muscle cells from TNF-alpha induced atrophy. PMID: 25760630
    8. Expression of USP19 correlates with that of MuRF1 and MAFbx/atrogin-1 in skeletal muscles PMID: 26048142
    9. MAFbx not only regulates protein degradation, but also reduces protein synthesis, exerting a dual role in regulating cardiac mass and preventing from cardiac hypertrophy. PMID: 24650875
    10. mechanical vibration strongly down-regulates atrophy genes myostatin and atrogin-1 both in vivo and in vitro. PMID: 22711460
    11. mTORC1 promotes denervation-induced muscle atrophy through a mechanism involving the activation of FoxO and E3 ubiquitin ligases. PMID: 24570486
    12. atrogin-1 promotes cardiomyocyte health through mediating the interplay between the ubiquitin/proteasome system and autophagy/lysosome system and its alteration promotes development of cardiomyopathies PMID: 24789905
    13. Smad3 expression is sufficient to stimulate atrogin-1 promoter activity, inhibit Akt/mTOR signaling and protein synthesis, and induce muscle fiber atrophy. PMID: 24002653
    14. The muscle wasting effects of the myostatin-atrogin-1 axis are not only limited to the degradation of MyoD and eukaryotic translation initiation factor 3 subunit f, but also encompass several other muscle proteins. PMID: 22673621
    15. Expression of MAFbx/Atrogin-1 and MuRF1 was significantly greater in the plantaris muscle than in the soleus muscle during the early stage of atrophy. PMID: 21901639
    16. C/EBPbeta-/- mice were resistant to Lewis lung cancer-induced atrogin1 upregulation and muscle wasting. Activation of the p38beta MAPK-C/EBPbeta signalling pathway appears to be a key component of cachexia. PMID: 21847090
    17. Downregulation of MAFbx inhibits cardiac hypertrophy in part through stabilization of IkappaB-alpha and inactivation of nuclear factor-kappaB PMID: 21617130
    18. Expression of MAFbx and Bnip3 was increased in hearts of mice in bearing colonic tumors. PMID: 21167183
    19. Myogenin is upregulated in skeletal muscle following denervation and regulates expression of the E3 ubiquitin ligases MuRF1 and atrogin-1, which promote muscle proteolysis and atrophy. PMID: 20887891
    20. Berberine impairs mitochonidrial function stimulating the expression of atrogin-1 without affecting phosphorylation of forkhead transcription factors. PMID: 20522589
    21. atrogin-1 specifically targets truncated M7t-cMyBP-C, but not WT-cMyBP-C, for proteasomal degradation and that MuRF1 indirectly reduces cMyBP-C levels by regulating the transcription of myosin heavy chain. PMID: 19850579
    22. Data show that slow in vivo release of GHRP-2 through minipump for 24h attenuated the thermal injury-induced increase in mRNA expression of IL-6 and of the E3 ubiquitin ligases, MuRF-1 and MAFbx, in rat skeletal muscle. PMID: 19577604
    23. Data indicate that the SCF(atrogin-1) ubiquitin ligase complex represses calcineurin by targeting it for ubiquitin-mediated proteolysis, leading to inhibition of cardiac hypertrophy in response to pathologic stimuli. PMID: 15489953
    24. MAFbx may play an important role in the course of muscle differentiation by determining the abundance of MyoD PMID: 15531760
    25. activation of atrogin-1 may be a common general adaptation in cells undergoing a rapid reduction in size. PMID: 17008454
    26. Results indicate that atrogin-1 does not affect the activity of Akt itself, but serves as a coactivator for members of the Forkhead family of transcription factors that function downstream of Akt. PMID: 17965779
    27. atrogin-1 may be a critical mediator of the muscle damage induced by statins. PMID: 17992259
    28. Targets the eukaryotic initiation factor 3 subunit 5 (eIF3-f) for ubiquitination and degradation by the proteasome. PMID: 18354498
    29. Although further study is required to establish the causal relationships, downregulation of MAFbx may have reduced MyoD degradation in favor of muscle regeneration. PMID: 18785187
    30. MAFbx targets MyoD for degradation in several models of skeletal muscle atrophy PMID: 19319192
    31. This study demenostrated that the astrogin1 protein is reduced in skeletal muscle of chronic spinal cord-injured patients. PMID: 19533653
    32. Results suggest that MAFbx functions as an F-box protein in the SCF complex for ubiquitination of myogenin. PMID: 19631210

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  • 亞細(xì)胞定位：
    Cytoplasm. Nucleus.
  • 組織特異性：
    Specifically expressed in cardiac and skeletal muscle.
  • 數(shù)據(jù)庫(kù)鏈接：