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  4. Mouse Fatty acid synthase(FASN) ELISA kit

Mouse Fatty acid synthase(FASN) ELISA kit

  • 中文名稱：
    小鼠脂肪酸合成酶(FASN)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL008435MO
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠脂肪酸合成酶(FASN)酶聯(lián)免疫試劑盒（CSB-EL008435MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的FASN含量。FASN（脂肪酸合酶）是參與內(nèi)源性脂肪酸合成的關(guān)鍵酶。其背景在于它在腫瘤等多種疾病中異常高表達(dá)。研究機(jī)制方面，主要聚焦于它促進(jìn)腫瘤細(xì)胞增殖、存活、遷移等過程，抑制 FASN 活性有望為相關(guān)疾病治療提供新策略。試劑盒檢測范圍為3.12 pg/mL-200 pg/mL，該產(chǎn)品適用于基礎(chǔ)科研中探究脂代謝調(diào)控機(jī)制、建立代謝性疾病動(dòng)物模型或腫瘤模型后FASN的動(dòng)態(tài)變化分析，以及藥物干預(yù)實(shí)驗(yàn)中靶向FASN通路的療效評估等研究場景，為揭示FASN在生理病理過程中的作用提供有效工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    FasnFatty acid synthase ELISA Kit; EC 2.3.1.85) [Includes: [Acyl-carrier-protein] S-acetyltransferase ELISA Kit; EC 2.3.1.38); [Acyl-carrier-protein] S-malonyltransferase ELISA Kit; EC 2.3.1.39); 3-oxoacyl-[acyl-carrier-protein] synthase ELISA Kit; EC 2.3.1.41); 3-oxoacyl-[acyl-carrier-protein] reductase ELISA Kit; EC 1.1.1.100); 3-hydroxyacyl-[acyl-carrier-protein] dehydratase ELISA Kit; EC 4.2.1.59); Enoyl-[acyl-carrier-protein] reductase ELISA Kit; EC 1.3.1.39); Oleoyl-[acyl-carrier-protein] hydrolase ELISA Kit; EC 3.1.2.14)] ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    3.12 pg/mL-200 pg/mL
  • 靈敏度：
    0.78 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse FASN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:100

    Average %

    91

    Range %

    86-97

    1:200

    Average %

    96

    Range %

    92-103

    1:400

    Average %

    85

    Range %

    81-89

    1:800

    Average %

    89

    Range %

    83-95

  • 回收率：

    The recovery of mouse FASN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    91

    87-95

    EDTA plasma (n=4)

    94

    90-99

  • 標(biāo)準(zhǔn)曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    200

    2.363

    2.503

    2.433

    2.302

    100

    2.025

    2.053

    2.039

    1.908

    50

    1.412

    1.501

    1.457

    1.326

    25

    0.936

    1.009

    0.973

    0.842

    12.5

    0.609

    0.586

    0.598

    0.467

    6.25

    0.354

    0.364

    0.359

    0.228

    3.12

    0.233

    0.245

    0.239

    0.108

    0

    0.125

    0.136

    0.131

    　

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    Fatty acid synthetase is a multifunctional enzyme that catalyzes the de novo biosynthesis of long-chain saturated fatty acids starting from acetyl-CoA and malonyl-CoA in the presence of NADPH. This multifunctional protein contains 7 catalytic activities and a site for the binding of the prosthetic group 4'-phosphopantetheine of the acyl carrier protein ([ACP]) domain.
  • 基因功能參考文獻(xiàn)：
    1. an unexpected link between FASN and cholesterol synthesis that appears to be required for TLR signal transduction and proinflammatory macrophage activation. PMID: 29463677
    2. FASN regulates the pathogenicity of Th17 cells. PMID: 28848043
    3. genetic deletion of FASN totally suppresses hepatocarcinogenesis driven by AKT and AKT/c-Met protooncogenes in mice. PMID: 28118080
    4. Data show that nuclear factor Y (NF-Y) binds to the inverted CCAAT element (ICE) in the Fatty acid synthase (Fasn) promoter specifically in refeeding states. PMID: 28281280
    5. study demonstrates that co-activation of AKT and c-Met induces hepatocellular carcinoma development that depends on the mTORC1/FASN pathway. PMID: 26857837
    6. Inhibition of G-protein-coupled Receptor Kinase 2 Prevents the Dysfunctional Cardiac Substrate Metabolism in Fatty Acid Synthase Transgenic Mice. PMID: 26670611
    7. These findings suggest that activation of TSHR directly inhibits FASN expression in mature adipocytes, possibly mediated by PKA and ERK PMID: 25655684
    8. UCP2-induced fatty acid synthase promotes NLRP3 inflammasome activation during sepsis PMID: 25574840
    9. FASN plays an important role in exercise-mediated cognitive enhancement, which might be associated to its role in modulating exercise-induced stimulation of neurogenesis. PMID: 24223732
    10. FASN is essential for the development, functional competence, and maintenance of the lactating mammary gland. PMID: 24668799
    11. Gamma-tocotrienol showed attenuation of triglyceride through effect on fatty acid synthase, sterol regulatory element-binding transcription factor 1, stearoyl CoA desaturase 1, and carnitine palmitoyl transferase 1A gene expression in Hepa 1-6 cells. PMID: 23727646
    12. Upregulation of host hepatic fatty acid synthase correlates with hepatitis B virus replication. PMID: 23675653
    13. Modulation of fatty acid synthase degradation by concerted action of p38 MAP kinase, E3 ligase COP1, and SH2-tyrosine phosphatase Shp2. PMID: 23269672
    14. fatty acid synthase (Fasn), the key enzyme of de novo lipogenesis, is highly active in adult neural stem and progenitor cells and conditional deletion of Fasn in mouse NSPCs impairs adult neurogenesis PMID: 23201681
    15. Kr-pok (kidney cancer-related POZ domain and Kruppel-like protein) activates transcription of the FASN gene. PMID: 22331133
    16. FAS deficiency blocked the generation of palmitoylated Mucin 2, which must be S-palmitoylated at its N terminus for proper secretion and function. PMID: 22341463
    17. Fatty acid synthase modulates homeostatic responses to myocardial stress PMID: 21757749
    18. These findings pertinent to the etiology of tumor metabolism also underscore the key role of the Sonic hedgehog-->E2F1-->FASN axis in regulating de novo lipid synthesis in cancers. PMID: 20890301
    19. Fatty acid synthase may serve as a new diagnostic marker or therapeutic target for the progression of nonalcoholic fatty liver disease PMID: 20606731
    20. FAS, which is induced by LXRalpha, may generate regulatory lipids that cause feedback inhibition of LXRalpha in macrophages. PMID: 20479009
    21. FAS is present and active in neurons in the hypothalamus PMID: 12376313
    22. role in fatty acid synthesis PMID: 12738878
    23. fatty acid synthase gene is regulated by SREBP PMID: 12897158
    24. Together, these results suggest that FAS generated signals may be essential to support preadipocyte differentiation. PMID: 15707987
    25. Insulin acutely reduces hepatic FAS activity by inducing phosphorylation of the carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) and its interaction with FAS. PMID: 16054098
    26. Inhibition of hypothalamic fatty acid synthase triggers rapid activation of fatty acid oxidation in skeletal muscle. PMID: 16203972
    27. AMP-activated protein kinase-induced Fatty acid synthase phosphorylation inhibits lipogenesis. PMID: 17635921
    28. 2 diametrically opposed proteins, fatty acid synthase (induced by feeding) and PPARalpha (induced by starvation), unexpectedly form an integrative sensory module in the central nervous system to orchestrate energy balance PMID: 17694178
    29. PPARalpha is required to mediate the response of Scd-1 and Fas gene expression to insulin and that this is normally achieved directly by activation of LXRalpha. PMID: 18079124
    30. Proto-oncogene FBI-1 (Pokemon) and SREBP-1 synergistically activate transcription of fatty-acid synthase gene PMID: 18682402
    31. Report effect of centrally administered C75, a fatty acid synthase inhibitor, on gastric emptying and gastrointestinal transit in mice. PMID: 18706411
    32. FASN activity is essential for B16-F10 melanoma cell proliferation and survival while its inactivation by Orlistat significantly reduces their metastatic spread PMID: 18770866

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  • 亞細(xì)胞定位：
    Cytoplasm. Melanosome.
  • 數(shù)據(jù)庫鏈接：