搡老女人多毛老妇女中国,日韩亚洲欧美中文高清在线,人妻少妇一区二区三区,色妞色综合久久夜夜,日本熟妇xxxx

Your Good Partner in Biology Research

  1. 首頁
  2. 試劑盒
  3. 酶聯(lián)免疫試劑盒
  4. Mouse Fetuin A ELISA Kit

Mouse Fetuin A ELISA Kit

  • 中文名稱：
    小鼠胎球蛋白A酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E14042m
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3900/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠胎球蛋白A酶聯(lián)免疫試劑盒（CSB-E14042m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的AHSG含量。AHSG即α2-HS糖蛋白，是肝臟合成的血漿糖蛋白。研究顯示它參與多種生理病理過程，如調(diào)節(jié)細(xì)胞生長、免疫反應(yīng)及礦物質(zhì)代謝等。其機(jī)制可能包括與細(xì)胞表面受體結(jié)合，影響信號通路傳導(dǎo)，進(jìn)而調(diào)節(jié)細(xì)胞的功能與行為。試劑盒檢測范圍為6.25 ng/mL-400 ng/mL，靈敏度為1.56 ng/mL。適用于科研領(lǐng)域探究小鼠模型中肝臟功能異常、糖脂代謝紊亂、心血管疾病或骨代謝相關(guān)機(jī)制。其寬泛的樣本兼容性可支持血清/血漿樣本的體液分析，或通過組織勻漿檢測特定器官的蛋白表達(dá)差異，為構(gòu)建疾病模型、評估干預(yù)效果提供可靠數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Ahsg ELISA kit; FetuaAlpha-2-HS-glycoprotein ELISA kit; Countertrypin ELISA kit; Fetuin-A ELISA kit
  • 縮寫：
    AHSG
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    6.25 ng/mL-400 ng/mL
  • 靈敏度：
    1.56 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

    　

    Intra-Assay Precision

    Inter-Assay Precision

    Sample

    1

    2

    3

    1

    2

    3

    n

    20

    20

    20

    20

    20

    20

    Mean(ng/ml)

    52.627

    50.900

    51.353

    52.901

    49.725

    50.990

    SD

    0.041

    0.035

    0.037

    0.054

    0.049

    0.047

    CV(%)

    5.216

    4.563

    4.793

    6.844

    6.499

    6.120

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse fetuin A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:10

    Average %

    95

    Range %

    87-103

    1:20

    Average %

    95

    Range %

    84-105

    1:40

    Average %

    90

    Range %

    83-103

    1:80

    Average %

    93

    Range %

    82-107

  • 回收率：

    The recovery of mouse fetuin A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    94

    90-98

    Heparin plasma (n=4)

    91

    85-96

  • 標(biāo)準(zhǔn)曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    Corrected

    400

    2.657

    2.704

    2.681

    2.547

    200

    1.905

    2.002

    1.954

    1.820

    100

    1.259

    1.279

    1.269

    1.135

    50

    0.714

    0.758

    0.736

    0.602

    25

    0.426

    0.442

    0.434

    0.300

    12.5

    0.361

    0.348

    0.355

    0.221

    6.25

    0.257

    0.244

    0.251

    0.117

    0

    0.134

    0.133

    0.134

    　

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Probably involved in differentiation.
  • 基因功能參考文獻(xiàn)：
    1. Downregulation of adiponectin in inflamed adipocyte by fetuin-A through the mediation of Wnt3a and PPARgamma is a new report. PMID: 27720679
    2. FETUA expression and ER stress markers in the liver and serum of HFD mice were decreased by melatonin treatment. In conclusion, melatonin can improve hepatic insulin resistance and hepatic steatosis through reduction in ER stress and the resultant AHSG expression. PMID: 29607540
    3. Impairment of energy sensors, SIRT1 and AMPK, in lipid induced inflamed adipocyte is regulated by Fetuin A. PMID: 29030114
    4. The growth plate slippage occurred in 70% of Ahsg-/- mice of both sexes around three weeks postnatal. PMID: 29088242
    5. AHSG is produced in bone, mainly in osteocytes and also show for the first time that its production is modulated by FGF23. PMID: 26476373
    6. These findings establish urinary Fetuin-A as a sensitive biomarker of the progression of autosomal dominant polycystic kidney disease. PMID: 25888842
    7. Plasma levels of the calcification inhibitor fetuin-A were significantly decreased in CKD animals compared to controls. PMID: 24994603
    8. fetuin-A regulates endochondral ossification through mineralization inhibition and lipid (hormone) binding [review] PMID: 23277412
    9. Our results show that CSF fetuin-A is a biomarker of disease activity and natalizumab response in multiple sclerosis PMID: 23439582
    10. lipid-induced FetA from adipocytes is an efficient chemokine for macrophage migration and polarization PMID: 23943623
    11. FetA, via its terminal galactoside moiety, directly binds Leu100-Gly123 & Thr493-Thr516 in Tlr4, fulfilling the requirement of an endogenous ligand for TLR4 through which lipids induce insulin resistance. PMID: 22842477
    12. Our results show that fetuin-A has a developmental rather than a structural role in murine bone. PMID: 23091616
    13. Fetuin-A-containing calciprotein particles facilitate the clearance of mineral debris by macrophages via SR-A. PMID: 22753077
    14. ER stress induced by high glucose and palmitate increases the expression of fetuin-A and further contributes to the development of insulin resistance PMID: 22619360
    15. fetuin-A is protective against lethal systemic inflammation partly by inhibiting active HMGB1 release PMID: 21347455
    16. Fetuin-A deficiency is associated with soft tissue calcification in mice and humans. PMID: 21659653
    17. breast cancer but also of other tumors whose initiation and promotion are under the tight control of TGF-beta. PMID: 20847285
    18. Fetuin-A expressions were negatively correlated with the liver pathological scores and TNFa levels in fulminant hepatic failure. PMID: 18578995
    19. alpha 2-HS glycoprotein/fetuin, a transforming growth factor-beta/bone morphogenetic protein antagonist, regulates postnatal bone growth and remodeling PMID: 11901155
    20. Improved insulin sensitivity and resistance to weight gain in knockout mice PMID: 12145157
    21. role as a systemically acting inhibitor of ectopic calcification PMID: 12897203
    22. Regulation of BMP-induced ectopic bone formation by Ahsg. PMID: 15885488
    23. Onset of malaria infection is delayed in fetuin-A-deficient mice compared to that in wild-type C57BL/6 mice challenged with Plasmodium berghei sporozoites PMID: 16113307
    24. Fetuin-A knockout (fetuin-KO) mice spontaneously develop widespread soft tissue calcification, including significant myocardial calcification, whereas larger arteries are spared. PMID: 16177000
    25. The absence of fetuin may contribute to the improvement of insulin sensitivity associated with aging. PMID: 17011519
    26. Fetuin-A deficiency, chronic kidney disease, and high phosphate diet act synergistically in the pathogenesis of extraosseous calcification. PMID: 17389622
    27. fetuin recognized by anti-LY-R antibodies is an immunogenic form associated only with the tumorigenic LY-R cells and might be involved in tumor progression in this lymphoma. PMID: 19194829
    28. inhibits pathologic calcification in both the soft tissue and vasculature, even in the setting of atherosclerosis PMID: 19389852
    29. inhibition of ectopic calcification PMID: 12556469
    30. inhibition of ectopic calcification PMID: 9395485

    顯示更多

    收起更多

  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Fetuin family
  • 組織特異性：
    Liver is the major site of synthesis, but fetuin is also expressed in limb buds and other extrahepatic tissues during development.
  • 數(shù)據(jù)庫鏈接：