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  4. Mouse Filaggrin(FLG) ELISA kit

Mouse Filaggrin(FLG) ELISA kit

  • 中文名稱：
    小鼠絲聚蛋白(FLG)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL008712MO
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠絲聚蛋白(FLG)酶聯(lián)免疫試劑盒（CSB-EL008712MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的FLG含量。FLG即絲聚蛋白，是皮膚角質形成細胞終末分化產(chǎn)物。它與皮膚屏障功能密切相關，編碼基因缺陷可導致皮膚屏障受損、水分丟失增加。其研究機制聚焦于闡釋在特應性皮炎等皮膚病中的作用，探尋基于FLG的診斷標記和治療靶點。試劑盒檢測范圍為3.12 pg/mL-200 pg/mL，適用于科研領域探索小鼠模型中FLG的表達調控機制，如研究皮膚屏障功能障礙、特應性皮炎等疾病的分子機制，或評估藥物干預、基因修飾對FLG蛋白水平的影響。為體外實驗或動物模型研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    FlgFilaggrin ELISA Kit; Fragment ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    3.12 pg/mL-200 pg/mL
  • 靈敏度：
    0.78 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse FLG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %91
    Range %86-95
    1:2Average %97
    Range %92-101
    1:4Average %88
    Range %86-95
    1:8Average %90
    Range %86-95
  • 回收率：
    The recovery of mouse FLG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9389-97
    EDTA plasma (n=4)10195-106
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    2002.290 2.433 2.362 2.197
    1001.503 1.390 1.447 1.282
    500.810 0.871 0.841 0.676
    250.573 0.610 0.592 0.427
    12.50.381 0.366 0.374 0.209
    6.250.288 0.274 0.281 0.116
    3.120.229 0.239 0.234 0.069
    00.163 0.167 0.165
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Aggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments during terminal differentiation of mammalian epidermis.
  • 亞細胞定位：
    Cytoplasmic granule.
  • 蛋白家族：
    S100-fused protein family
  • 組織特異性：
    Expressed in the granular layer of the epidermis.
  • 數(shù)據(jù)庫鏈接：