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  4. Mouse GDNF family receptor alpha-like(GFRAL) ELISA kit

Mouse GDNF family receptor alpha-like(GFRAL) ELISA kit

  • 中文名稱(chēng)：
    小鼠GDNF家族α樣受體(GFRAL)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL009383MO
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠GDNF家族α樣受體(GFRAL)酶聯(lián)免疫試劑盒（CSB-EL009383MO）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的GFRAL含量。GFRAL是一個(gè)重要靶點(diǎn)。它與機(jī)體能量代謝等生理過(guò)程密切相關(guān)。研究機(jī)制上，其能參與特定信號(hào)通路，可能成為調(diào)節(jié)能量平衡的關(guān)鍵環(huán)節(jié)。目前圍繞它正開(kāi)展眾多科研工作，有望為肥胖癥等代謝疾病治療帶來(lái)新策略。試劑盒檢測(cè)范圍為25 pg/mL-1600 pg/mL，本試劑盒通過(guò)高特異性配對(duì)抗體實(shí)現(xiàn)目標(biāo)蛋白的精準(zhǔn)捕獲與信號(hào)放大，適用于基礎(chǔ)科研領(lǐng)域探索GFRAL在代謝性疾病動(dòng)物模型中的動(dòng)態(tài)變化、評(píng)估特定組織或細(xì)胞中受體表達(dá)差異，以及篩選調(diào)控GFRAL通路的藥物或生物活性分子。產(chǎn)品嚴(yán)格驗(yàn)證了線(xiàn)性范圍與靈敏度指標(biāo)，可滿(mǎn)足體外實(shí)驗(yàn)中低豐度蛋白的穩(wěn)定檢測(cè)需求，為代謝機(jī)制研究、神經(jīng)內(nèi)分泌調(diào)控及疾病模型構(gòu)建提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    Gfral ELISA Kit; Gral ELISA Kit; GDNF family receptor alpha-like ELISA Kit
  • 縮寫(xiě)：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類(lèi)型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測(cè)范圍：
    25 pg/mL-1600 pg/mL
  • 靈敏度：
    6.25 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Cell Biology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線(xiàn)性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse GFRAL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 92
    Range % 88-98
    1:2 Average % 101
    Range % 97-107
    1:4 Average % 94
    Range % 90-98
    1:8 Average % 97
    Range % 92-101
  • 回收率：
    The recovery of mouse GFRAL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 95 89-100
    EDTA plasma (n=4) 88 84-92
  • 標(biāo)準(zhǔn)曲線(xiàn)：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    1600 1.859 1.952 1.906 1.766
    800 1.258 1.260 1.259 1.119
    400 0.720 0.734 0.727 0.587
    200 0.466 0.489 0.478 0.338
    100 0.315 0.328 0.322 0.182
    50 0.229 0.235 0.232 0.092
    25 0.172 0.176 0.174 0.034
    0 0.138 0.141 0.140 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses. Upon interaction with its ligand, GDF15, interacts with RET and induces cellular signaling through activation of MAPK- and AKT- signaling pathways.
  • 基因功能參考文獻(xiàn)：
    1. isolating GFRAL as the receptor for GDF15-induced anorexia and weight loss, the identification of a mechanistic basis for the non-homeostatic regulation of neural circuitry by a peripheral signal associated with tissue damage and stress; findings provide opportunities to develop therapeutic agents for the treatment of disorders with altered energy demand PMID: 28953886
  • 亞細(xì)胞定位：
    Cell membrane; Single-pass membrane protein; Extracellular side.
  • 蛋白家族：
    GDNFR family
  • 組織特異性：
    Expressed in the brainstem, restricted to cells in the area postrema and the immediately adjacent region of the nucleus tractus solitarius.
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: mmu:404194

    UniGene: Mm.213862