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  4. Mouse Galectin 3(GAL-3) ELISA Kit

Mouse Galectin 3(GAL-3) ELISA Kit

  • 中文名稱：
    小鼠半乳糖凝集素-3(Gal-3)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E14296m
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    小鼠半乳糖凝集素-3(Gal-3)酶聯(lián)免疫試劑盒（CSB-E14296m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的LGALS3含量。LGALS3 是一個重要靶點。它在細胞黏附、增殖、凋亡等多種生理病理過程中發(fā)揮作用。相關研究圍繞其與腫瘤發(fā)生發(fā)展、免疫調節(jié)等機制展開，探索通過抑制其活性來開發(fā)新型治療藥物，為癌癥等疾病的治療帶來新希望。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，適用于小鼠疾病模型構建、藥物干預研究及分子機制探索等科研領域，特別針對心血管疾病、肝纖維化或腫瘤微環(huán)境研究中Gal - 3的動態(tài)變化監(jiān)測本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    Lgals3 ELISA Kit; Galectin-3 ELISA Kit; Gal-3 ELISA Kit; 35 kDa lectin ELISA Kit; Carbohydrate-binding protein 35 ELISA Kit; CBP 35 ELISA Kit; Galactose-specific lectin 3 ELISA Kit; IgE-binding protein ELISA Kit; L-34 galactoside-binding lectin ELISA Kit; Laminin-binding protein ELISA Kit; Lectin L-29 ELISA Kit; Mac-2 antigen ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.312 ng/mL-20 ng/mL
  • 靈敏度：
    0.078 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Cell Biology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse GAL-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 91  
    Range % 85-103  
    1:2 Average % 90  
    Range % 82-99  
    1:4 Average % 92  
    Range % 86-100  
    1:8 Average % 92  
    Range % 89-103  
  • 回收率：
    The recovery of mouse GAL-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 90-101  
    EDTA plasma (n=4) 97 91-105  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    20 2.852 2.842 2.847 2.703  
    10 2.207 2.280 2.244 2.100  
    5 1.357 1.384 1.371 1.227  
    2.5 0.841 0.862 0.852 0.708  
    1.25 0.475 0.458 0.467 0.323  
    0.625 0.283 0.297 0.290 0.146  
    0.312 0.231 0.244 0.238 0.094  
    0 0.142 0.146 0.144    
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. Together with TRIM16, coordinates the recognition of membrane damage with mobilization of the core autophagy regulators ATG16L1 and BECN1 in response to damaged endomembranes.
  • 亞細胞定位：
    Cytoplasm. Nucleus. Secreted.
  • 組織特異性：
    The highest levels are found in activated macrophages.
  • 數據庫鏈接：