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  3. 酶聯(lián)免疫試劑盒
  4. Mouse Gastrin ELISA Kit
  • 中文名稱：
    小鼠胃泌素酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E12924m
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠胃泌素酶聯(lián)免疫試劑盒（CSB-E12924m）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的GAST含量。小鼠胃泌素是一種由胃竇及小腸黏膜的G細(xì)胞分泌的激素，對(duì)胃酸分泌和胃腸道黏膜生長(zhǎng)有促進(jìn)作用。研究發(fā)現(xiàn)，胃泌素過量表達(dá)與多種腫瘤的發(fā)生發(fā)展密切相關(guān)，其作用機(jī)制主要通過CCK受體介導(dǎo)，影響細(xì)胞增殖、遷移和凋亡等過程。試劑盒檢測(cè)范圍為1.25 pg/mL-80 pg/mL，適用于基礎(chǔ)醫(yī)學(xué)研究中探索胃腸道疾病模型、藥物干預(yù)對(duì)胃泌素分泌的影響，或評(píng)估胃腸功能調(diào)節(jié)機(jī)制等領(lǐng)域本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Gast ELISA Kit; Gas ELISA Kit; Gastrin [Cleaved into: Gastrin-71 ELISA Kit; G71); Big gastrin ELISA Kit; Gastrin-34 ELISA Kit; G34); Gastrin] ELISA Kit
  • 縮寫：
    GAST
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    1.25 pg/mL-80 pg/mL
  • 靈敏度：
    0.31 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse Gastrin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %93
    Range %86-99
    1:2Average %98
    Range %92-105
    1:4Average %94
    Range %88-97
    1:8Average %88
    Range %83-95
  • 回收率：
    The recovery of mouse Gastrin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9387-99
    EDTA plasma (n=4)9895-103
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    802.785 2.650 2.718 2.555
    402.384 2.375 2.380 2.217
    201.689 1.752 1.721 1.558
    100.974 1.030 1.002 0.839
    50.575 0.532 0.554 0.391
    2.50.362 0.357 0.360 0.197
    1.250.247 0.258 0.253 0.090
    00.161 0.165 0.163 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

平均分:
5.0分 - 1 個(gè)評(píng)價(jià)

樣品類型：血清

樣品信息：小鼠

稀釋比：沒有稀釋

產(chǎn)品評(píng)價(jià)： 我用CSB-E12924m檢測(cè)正常血清，其od值為1.72/2.081/1.893/1.946/2.016/1.793可以正常檢測(cè)出

By 張老師

靶點(diǎn)詳情

  • 功能：
    Gastrin stimulates the stomach mucosa to produce and secrete hydrochloric acid and the pancreas to secrete its digestive enzymes. It also stimulates smooth muscle contraction and increases blood circulation and water secretion in the stomach and intestine.
  • 基因功能參考文獻(xiàn)：
    1. Gastrin induces nuclear export and proteasome degradation of menin in duodenal glial cells. PMID: 28859856
    2. PTHLH is a gastrin-regulated growth factor that might contribute to gastric epithelial cell homeostasis. PMID: 28408643
    3. Renal-selective silencing of Gast by the renal subcapsular injection of Gast siRNA in BALB/c mice decreases renal dopamine production and increases blood pressure. PMID: 27780818
    4. Gastrin is expressed in insulin secreting cells of adult diabetic mice. PMID: 27864307
    5. increased circulating concentrations of progastrin provide a physiological advantage against systemic hypoxia in mice PMID: 25394662
    6. Ihh signaling mediates gastrin-induced proliferation of epithelial cells in stomachs of adult mice. PMID: 24859162
    7. Data indicate that epithelial Gli2 expression was sufficient to stimulate Il-1beta expression, repress Gast gene expression and increase proliferation, leading to antral hyperplasia. PMID: 23110168
    8. gastrins play an important direct role, unrelated to their ability to stimulate acid secretion, in hematopoiesis under conditions of iron deficiency. PMID: 21652729
    9. Initiation of antral gastric cancer is associated with progressive epigenetic silencing of TFF1, which can be suppressed by the hormone gastrin. PMID: 21111741
    10. Regulation of mouse gastrin gene in vivo. Mechanisms involved in transcriptional control of the gastrin gene. PMID: 21051525
    11. RegI and gastrin may synergistically regulate gastric mucosal proliferation during certain pathological settings like wound healing PMID: 19969026
    12. To examine the importance of gastrin or its receptors during development in general and for gastric physiology specifically both have been knocked out. PMID: 11713979
    13. stimulates the growth of gastric pit cell precursors by inducing its own receptors. PMID: 11804858
    14. Endocrine gastrin may have a role in the colonic adenoma carcinoma sequence. Hyper-gastrinaemia may promote proliferation of human colonic adenomas that express CCK-2 receptor isoforms. PMID: 12189558
    15. Parietal cells were found to be significantly smaller in the mutant cell preparations, suggesting that gastrin stimulation modulates parietal cell morphology. PMID: 12388185
    16. essential physiologic role for gastrin in glucose homeostasis; however, the gastrin gene is not essential for murine islet development or the adaptive islet proliferative response to beta-cell injury. PMID: 14517799
    17. gastrin promoter is activated by transforming growth factor-beta/Smad and Wnt signaling pathways PMID: 15292219
    18. gastrin expression, possibly contributing to neoplastic progression, is induced by synergistic Wnt and Ras signaling pathway PMID: 16139800
    19. The aim of this study was to evaluate changes in the acid-secreting parietal cell in gastrin-deficient (GAS-KO) mice. PMID: 16278279
    20. Hypergastrinemia increases regeneration of intestinal epithelia following diverse forms of injury. PMID: 16618411
    21. gastrin can induce apoptosis in gastric epithelial cells and contribute to the development of gastric carcinogenesis PMID: 16894354
    22. Gastrin concentrations are increased in the plasma and gastric mucosa of Hfe(-/-) mice, and in the sera of humans with HFE-related hemochromatosis. PMID: 17064691
    23. Gastrin regulates TFF2 transcription through a GC-rich DNA-binding site and a protein kinase dependent pathway. PMID: 17332476
    24. Identification of ezrin as a target of gastrin in immature mouse gastric parietal cells. PMID: 18567601
    25. Results suggest that RNAi targeting of gastrin could serve as an effective treatment for pancreatic cancer. PMID: 19465883

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Gastrin/cholecystokinin family
  • 組織特異性：
    Abundantly expressed in the stomach and duodenum. Low levels in brain, ovary and pancreas.
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: mmu:14459

    STRING: 10090.ENSMUSP00000017309

    UniGene: Mm.4767