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  4. Mouse Gastrin-releasing peptide(GRP) ELISA kit

Mouse Gastrin-releasing peptide(GRP) ELISA kit

  • 中文名稱：
    小鼠胃泌素釋放肽(GRP)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL009940MO
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠胃泌素釋放肽(GRP)酶聯(lián)免疫試劑盒（CSB-EL009940MO）檢測血清、血漿、組織勻漿樣本中的GRP含量。GRP即胃泌素釋放肽，是一種神經(jīng)遞質(zhì)。它在多種生理過程中發(fā)揮作用，還與腫瘤發(fā)生發(fā)展密切相關(guān)。研究機(jī)制上，GRP與其受體結(jié)合，能激活下游信號通路，促進(jìn)細(xì)胞增殖、抑制凋亡等，因此成為腫瘤等疾病治療的潛在靶點(diǎn)。試劑盒檢測范圍為39 pg/mL-2500 pg/mL，適用于胃腸功能研究、神經(jīng)生物學(xué)實(shí)驗(yàn)及腫瘤相關(guān)基礎(chǔ)科研領(lǐng)域，滿足體外實(shí)驗(yàn)中對GRP表達(dá)動態(tài)的定量分析需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Grp ELISA Kit; Gastrin-releasing peptide ELISA Kit; GRP) [Cleaved into: Neuromedin-C ELISA Kit; GRP-10)] ELISA Kit
  • 縮寫：
    GRP
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    39 pg/mL-2500 pg/mL
  • 靈敏度：
    9.75 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse GRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 98  
    Range % 92-102  
    1:2 Average % 98  
    Range % 91-106  
    1:4 Average % 95  
    Range % 89-98  
    1:8 Average % 96  
    Range % 92-104  
  • 回收率：
    The recovery of mouse GRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 89-98  
    EDTA plasma (n=4) 89 82-96  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    2500 2.738 2.616 2.677 2.512  
    1250 2.158 2.010 2.084 1.919  
    625 1.390 1.368 1.379 1.214  
    312.5 0.866 0.886 0.876 0.711  
    156.25 0.560 0.542 0.551 0.386  
    78.125 0.351 0.363 0.357 0.192  
    39.063 0.256 0.250 0.253 0.088  
    0 0.162 0.167 0.165 　  
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點(diǎn)詳情

  • 功能：
    Stimulates the release of gastrin and other gastrointestinal hormones. Contributes to the perception of prurient stimuli and to the transmission of itch signals in the spinal cord that promote scratching behavior. Contributes primarily to nonhistaminergic itch sensation. Contributes to long-term fear memory, but not normal spatial memory. Contributes to the regulation of food intake.
  • 基因功能參考文獻(xiàn)：
    1. These data suggest that GRPR expression in fibroblast-like synoviocytes (FLS)and that exogenous GRP are able to activate FLS invasion. This effect occurs at least in part through the AKT activation. Therefore, understanding of the GRP/GRPR pathway could be relevant in the development of FLS-targeted therapy for Rheumatoid arthritis . PMID: 28733141
    2. Fos and phosphorylation of extracellular signal-regulated kinases (ERK) were used to look for evidence that interneurons expressing GRP were activated following intradermal injection of chloroquine. PMID: 27270268
    3. GrpmRNA and protein are upregulated in dorsal root ganglias, but not in the spinal cord, of mice with chronic itch. PMID: 27068287
    4. GRP is expressed by a distinct population of excitatory interneurons in laminae I-II that are likely to be involved in the itch pathway PMID: 25496164
    5. BNP-NPRA signaling is involved in both itch and pain and does not function upstream of the GRP-GRPR dedicated neuronal pathway. PMID: 24438367
    6. nonamidated peptides derived from the C terminus of pro-GRP are expressed in significant quantities in colorectal cancer cell lines PMID: 22202166
    7. GRP delays the phase of the clock during the early night by prolonging day-like membrane properties of SCN cells PMID: 21454290
    8. The GRP receptor system does not mediate kappa opioid receptor antagonist (GNTI)-induced scratching. The kappa opioid system is involved, at least in part, in the scratch suppressing activity of the kappa opioid receptor agonist nalfurafine. PMID: 21126550
    9. These studies demonstrate that (64)Cu-SarAr-SA-Aoc-bombesin(7-14) and (64)Cu-SarAr-SA-Aoc-GSG-bombesin(7-14) bound with high affinity to GRPR-expressing cells. PMID: 21321264
    10. A general possible effect of GRP on cortical inhibitory transmission, was shown. PMID: 20836873
    11. GRP/GRP-R play a transient and non-critical role in intestinal development PMID: 11960700
    12. GRP has a role in regulation of fear and memory for fear in the mouse amygdala PMID: 12526815
    13. Potential new role for gastrin-releasing peptide -BB2 signaling within the circadian clock. PMID: 16319315
    14. New evidence for the occurrence of marked effects of bombesin/gastrin-releasing peptide concerning both the articular chondrocytes and the inflammatory process is obtained in this study PMID: 17911468
    15. GRP nerve fibers were markedly high in the epidermis of atopic dermatitis mice PMID: 19571818

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  • 亞細(xì)胞定位：
    Secreted. Cytoplasmic vesicle, secretory vesicle lumen.
  • 蛋白家族：
    Bombesin/neuromedin-B/ranatensin family
  • 組織特異性：
    Detected in the suprachiasmatic nucleus in the hypothalamus. Detected in peptidergic dorsal root ganglion neurons (at protein level). Highly expressed both in the lateral nucleus of the amygdala, and in regions sending synaptic projections to the lateral
  • 數(shù)據(jù)庫鏈接：