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中文名稱:小鼠胰高血糖素(GC)酶聯免疫試劑盒
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貨號:CSB-E15775m
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規(guī)格:96T/48T
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價格:¥3600/¥2500
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其他:
產品詳情
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產品描述:小鼠胰高血糖素(GC)酶聯免疫試劑盒(CSB-E15775m)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的GCG含量。GCG(胰高血糖素)是一種重要靶點。它在血糖調節(jié)等生理過程中起關鍵作用。研究機制上,GCG通過與受體結合,激活細胞內信號通路,影響糖原分解、糖異生等代謝途徑。圍繞它開展的研究有助于深入了解代謝調控機制及相關疾病防治。試劑盒檢測范圍為1.56 pg/mL-100 pg/mL,適用于能量代謝調控、糖代謝機制及糖尿病相關動物模型的科學研究;能夠滿足體外實驗中GC動態(tài)監(jiān)測、組織分布分析及干預效果評價等科研需求,為代謝性疾病機制研究提供可靠工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
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別名:GcgGlucagon [Cleaved into: Glicentin; Glicentin-related polypeptide ELISA kit; GRPP); Oxyntomodulin ELISA kit; OXM ELISA kit; OXY); Glucagon; Glucagon-like peptide 1 ELISA kit; GLP-1); Glucagon-like peptide 1(7-37 ELISA kit; GLP-1(7-37)); Glucagon-like peptide 1(7-36 ELISA kit; GLP-1(7-36)); Glucagon-like peptide 2 ELISA kit; GLP-2)] ELISA kit
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縮寫:
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Uniprot No.:
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種屬:Mus musculus (Mouse)
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樣本類型:serum, plasma, tissue homogenates
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檢測范圍:1.56 pg/mL-100 pg/mL
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靈敏度:0.39 pg/mL
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反應時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領域:Metabolism
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse GC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:5 Average % 92 Range % 88-98 1:10 Average % 97 Range % 93-110 1:20 Average % 95 Range % 88-100 1:40 Average % 95 Range % 82-104 -
回收率:
The recovery of mouse GC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 94 85-99 EDTA plasma (n=4) 95 86-102 -
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 100 2.564 2.592 2.578 2.489 50 1.439 1.488 1.464 1.375 25 0.805 0.792 0.799 0.710 12.5 0.421 0.432 0.427 0.338 6.25 0.214 0.223 0.219 0.130 3.12 0.146 0.150 0.148 0.059 1.56 0.102 0.105 0.104 0.015 0 0.088 0.090 0.089 -
數據處理:
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貨期:3-5 working days
引用文獻
- Pathophysiological characterization of the ApoE?/?; db/db mouse: A model of diabetes and atherosclerosis M Paniagua-Sancho, AG Casanova,Methods,2025
- TM4SF5-mediated abnormal food-intake behavior and apelin expression facilitate non-alcoholic fatty liver disease features YD Pinanga,iScience,2023
- Cholic acid exposure during late pregnancy causes placental dysfunction and fetal growth restriction by reactive oxygen species-mediated activation of placental GCN2/eIF2α pathway S Lin,FASEB journal,2023
- Effects of long-term administration of theasinensin A on healthy C57BL/6J mice: Enhancing the function of epididymal white adipose tissue and regulating the colonic microenvironment W Xu,Food Chemistry,2022
- Systematic evaluation of antimicrobial food preservatives on glucose metabolism and gut microbiota in healthy mice P Li,NPJ science of food,2022
- The effects of metformin and alendronate in attenuating bone loss and improving glucose metabolism in diabetes mellitus mice Q Zhou,Aging (Albany NY),2022
- Environmental cadmium exposure during pregnancy causes diabetes-like phenotypes in mouse offspring: association with oxidative stress in the fetal liver Yong-Wei Xiong,Science Of The Total Environment,2021
- Si-Miao-Yong-An decoction preserves cardiac function and regulates GLC/AMPK/NF-κB and GLC/PPARα/PGC-1α pathways in diabetic mice L Li,Biomedicine & Pharmacotherapy,2020
- The RNA-binding protein, HuD regulates proglucagon biosynthesis in pancreatic α cells S Ahn,Biochemical and Biophysical Research Communications,2020
- Melatonin ameliorates SGLT2 inhibitor-induced diabetic ketoacidosis by inhibiting lipolysis and hepatic ketogenesis in type 2 diabetic mice Jae-Hyung Park, et al,Journal of Pineal Research,2019
- Hyperglycaemia Stress-Induced Renal Injury is Caused by Extensive Mitochondrial Fragmentation, Attenuated MKP1 Signalling, and Activated JNK-CaMKII-Fis1 Biological Axis Zhang Y, et al,Cellular physiology and biochemistry,2018
- DUSP1 recuses diabetic nephropathy via repressing JNK-Mff-mitochondrial fission pathways Junqin Sheng.et al,Journal of cellular physiology,2018
- SIRT3 Facilitates Amniotic Fluid Stem Cells to Repair Diabetic Nephropathy Through Protecting Mitochondrial Homeostasis by Modulation of Mitophagy Feng J.et al,Cellular Physiology and Biochemistry,2018
- 6-Shogaol ameliorates diabetic nephropathy through anti-inflammatory, hyperlipidemic, anti-oxidative activity in db/db mice Xu Y.et al,Biomed Pharmacother,2017
- Homoarginine supplementation improves blood glucose in diet-induced obese mice Stockebrand M. et al,Amino Acids. ,2015
- Plasma biomarkers as predictors of recurrence of atrial fibrillation. Lewicka E. et al,Pol Arch Med Wewn,2015
產品評價
樣品類型:血清
樣品信息:小鼠
稀釋比:其他 1:10
產品評價: 我用CSB-E15775m檢測自發(fā)性T2DM小鼠db/db小鼠,稀釋比例為1:10,稀釋比例正好,濃度合適,產品真的太好用啦!
By 丁老師
相關產品
靶點詳情
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功能:Plays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin, raises plasma glucose levels in response to insulin-induced hypoglycemia. Plays an important role in initiating and maintaining hyperglycemic conditions in diabetes.; Potent stimulator of glucose-dependent insulin release. Also stimulates insulin release in response to IL6. Plays important roles on gastric motility and the suppression of plasma glucagon levels. May be involved in the suppression of satiety and stimulation of glucose disposal in peripheral tissues, independent of the actions of insulin. Has growth-promoting activities on intestinal epithelium. May also regulate the hypothalamic pituitary axis (HPA) via effects on LH, TSH, CRH, oxytocin, and vasopressin secretion. Increases islet mass through stimulation of islet neogenesis and pancreatic beta cell proliferation. Inhibits beta cell apoptosis (Probable).; Stimulates intestinal growth and up-regulates villus height in the small intestine, concomitant with increased crypt cell proliferation and decreased enterocyte apoptosis. The gastrointestinal tract, from the stomach to the colon is the principal target for GLP-2 action. Plays a key role in nutrient homeostasis, enhancing nutrient assimilation through enhanced gastrointestinal function, as well as increasing nutrient disposal. Stimulates intestinal glucose transport and decreases mucosal permeability.; Significantly reduces food intake. Inhibits gastric emptying in humans. Suppression of gastric emptying may lead to increased gastric distension, which may contribute to satiety by causing a sensation of fullness.; May modulate gastric acid secretion and the gastro-pyloro-duodenal activity. May play an important role in intestinal mucosal growth in the early period of life.
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基因功能參考文獻:
- Data suggest that Tas1r2 and Tas1r3 are involved in regulation of Glp1 secretion in enteroendocrine cells; 3DG (3-deoxyglucosone) attenuates high glucose-stimulated Glp1 secretion by antagonizing Tas1r2/Tas1r3 subunits and downstream cAMP signaling. (Tas1r2 = sweet taste receptor subunit Tas1r2; Tas1r3 = sweet taste receptor subunit Tas1r3; Glp1 = glucagon-like peptide-1) PMID: 29277113
- GPR119 is the oleoyl-lysophosphatidylinositol receptor that is required for GLP-1 secretion in enteroendocrine cells. PMID: 29883799
- data show that the CREB/CRTC2-dependent transcriptional pathway is critical for regulating glucose homeostasis by controlling production of GLP-1 from the L cells at the level of transcription, maturation, and exocytosis. PMID: 29118086
- the results of the present study indicated that GLP1 may be a promising target for the development of novel therapeutic strategies for HGinduced nephropathy, and may function through the activation of SIRT1 PMID: 29845208
- this study, we investigated whether glucagon and glucagon-like peptide-1 (GLP-1), hormones produced by alpha cells, contribute to insulin secretion in INS-1 cells, a beta cell line. Co-treatment with glucagon and exendin-4 (Ex-4), a GLP-1 receptor agonist, additively increased glucose-stimulated insulin secretion in INS-1 cells PMID: 29725251
- results show that glucagon controls gene expression and metabolic zonation in the liver through a counterplay with the Wnt/beta-catenin signaling pathway. PMID: 29555772
- Data (including data from studies using transgenic and knockout mice) suggest that Glp1/Glp1r signaling in insulin-secreting cells plays important role in development of glucose intolerance in obesity; however, Glp1r is not required in insulin-secreting cells for improvement in glucose intolerance after weight loss due to bariatric surgery (here, vertical sleeve gastrectomy). PMID: 29759973
- Data suggest that metabolism of glutamine and related analogs by Gdh in intestinal L-cells explains why Glp1 secretion, but not that of insulin by pancreatic beta-cells, is activated by these secretagogues. (Gdh = glutamate dehydrogenase; Glp1 = glucagon-like peptide 1) PMID: 29229616
- Glucokinase governs an alpha-cell metabolic pathway that suppresses secretion at or above normoglycemic levels; abnormal suppression of glucagon secretion deregulates hepatic glucose metabolism and, over time, induces a pre-diabetic phenotype. PMID: 29416045
- in colonic crypt cultures, the GLP-1 secretion induced by such Gq + Gs GPR40 agonists is indeed inhibited by blockers of both Gq and Gs and is eliminated by combining these. PMID: 27908836
- Enteric GLP-1 activates NO production by enteric neurons that is impaired in type 2 diabetes. Gut microbiota dysbiosis induces enteric neuropathy. Gut microbiota dysbiosis is responsible for the GLP-1 resistance. PMID: 28467926
- of glucagon-like peptide-1 in vagotomized mice may prevent VLDL overproduction and insulin resistance induced by high-fat diet. PMID: 29074588
- beta-cell function, plasma active GLP-1 levels, the GLP-1R pathway in beta cells and L cell differentiation, were investigated. PMID: 27436347
- The role of syntaxin 1A in GLP1 release from intestinal cells as a response to external stimuli is reported. PMID: 28596237
- GCG neurons likely stimulate separate populations of downstream cells to produce a change in food intake and glucose homeostasis and that these effects depend on the metabolic state of the animal. PMID: 28218622
- Together, our data indicate effects of AgoPAMs that go beyond glucose lowering previously observed with GPR40 partial agonist treatment with additional potential for weight loss. PMID: 28292762
- pancreatic reactivation of Gcg fully restored the effect of exendin-[9-39] to impair both oral and intraperitoneal glucose tolerance. PMID: 28325479
- These findings suggest that TRPV2 activation via actin reorganization induced by Gq and G12/13 signaling is involved in LPI-stimulated GLP-1 secretion in enteroendocrine L cells. PMID: 28533434
- These results show novel ex vivo effects of rebaudioside A on enteroendocrine cells of the mouse small intestine and highlight potentially new applications for rebaudioside A in metabolic diseases. PMID: 27798332
- critical for the regulation of glucagon secretion in response to glucose in obesity PMID: 27547850
- These results strongly suggest that incretins upregulate the TNF-alpha-stimulated IL6 synthesis in osteoblasts, and that the amplifying effect of incretin is exerted via reducing the IkappaB/NFkappaB pathway through the adenylyl cyclase-cAMP system. PMID: 28204823
- These results indicated that 5rolGLP-HV had dual-function in treating diabetes and preventing thrombosis. PMID: 26780765
- Glucagon-like peptide-1 regulates calcium homeostasis and electrophysiological activities in cultured cardiomyocytes. PMID: 26930508
- GLP-1 release is altered in intestinal cultures from a high fat diet-fed mice. PMID: 26145551
- Data show that ginsenoside Rg3 stimulated glucagon-like peptide-1 (GLP-1) secretion in NCI-H716 enteroendocrine cells. PMID: 26675132
- GPR119 in L-cells plays a key role in oral lipid-triggered GLP-1 secretion. PMID: 26144594
- AMPK antagonizes hepatic glucagon signalling via phosphorylation-induced PDE4B activation PMID: 26952277
- Deletion of AMPK alpha 1 and alpha 2 in proglucagon-expressing cells results in increased L-cell mass and elevated circulating GLP-1 levels. PMID: 27010458
- The results suggest that TGR5 activation mediates cross-talk between alpha- and beta-cells by switching from glucagon to GLP-1 to restore beta- cell mass and function under hyperglycemic conditions. PMID: 26757816
- the acute combined administration of the strongly insulinotropic GLP-1 and glucagon, both in vivo and in vitro, did not induce any additive or synergistic action on glucose-stimulated insulin secretion. PMID: 26119909
- Data (including data from studies in transgenic mice) suggest neurotensin/Nts, GLP-1, and peptide YY are closely co-expressed and co-secreted within enteroendocrine cells in ileum mucosa; however, Nts is stored in distinct/separate secretory granules. PMID: 26469136
- CEACAM2 regulates insulin secretion, at least in part, by a GLP-1-mediated mechanism, independent of confounding metabolic factors. PMID: 26586918
- These data suggest that GLP-1 released from NTS neurons can reduce highly palatable food intake by suppressing mesolimbic DA signaling. PMID: 26212334
- Neuronostatin acts via GPR107 to increase cAMP-independent PKA phosphorylation and proglucagon mRNA accumulation in pancreatic alpha-cells. PMID: 26561648
- Data indicate that GCGKO mice, lack all proglucagon-derived peptides, including glucagon and GLP-1 are animal model for studying the development, pathogenesis, and metastasis of pancreatic neuroendocrine tumors (panNETs). PMID: 26192435
- Data show that ileal sections were costained for glucagon-like peptide-1 (GLP-1) and tumor necrosis factor receptor TNFR1. PMID: 26270730
- Data show that the action of bile acids on -like peptide-1 (GLP-1) secretion is predominantly mediated by G protein-coupled bile acid receptor GPBAR1 (TGR5) located on the basolateral L-cell membrane. PMID: 26280129
- The PKC-dependent effect of GLP-1 on membrane potential and electrical activity was mediated by activation of Na(+)-permeable TRPM4 and TRPM5 channels by mobilization of intracellular Ca(2+) from thapsigargin-sensitive Ca(2+) stores PMID: 26571400
- It was suggested that increased ileal GPR119 is a potential mechanism by which GLP-1 secretion is enhanced in apoA-IV-/- mice. PMID: 26294669
- Hypoxia decreases GLP-1 secretion from the GLUTag cell line, and our findings suggest that the postprandial decrease in oxygen tension in the intestine attenuates GLP-1 secretion. PMID: 25832631
- In mice, food intake stimulates oxyntomodulin secretion from the gut, which resets liver transcription rhythms via induction of the core clock genes Per1 and 2. PMID: 25821984
- Suggest that endogenous GLP-2 may act as a protective factor against the dysregulation of the glucose metabolism that occurs in mice fed a high fat diet. PMID: 25967277
- synaptotagmin-7 is directly activated by GLP-1 signaling and may serve as a drug target for boosting insulin secretion. PMID: 26216970
- Glucocorticoid receptor activation in GLP-1-producing cells will diminish the secretory responsiveness of these cells to subsequent carbohydrate stimulation leading to diabetes. PMID: 25853863
- The patterns of colocalisation of the K cell marker, glucagon-like insulinotropic peptide, and the L cell markers, glucagon like peptide-1 and peptide YY, in enteroendocrine cells of the small intestine and colon of mouse and pig, were investigated. PMID: 25378285
- These data indicate that GLP-1 but not GIP is a key mediator of beta cell mass expansion and related adaptations in pregnancy, triggered in part by generation of intra-islet GLP-1. PMID: 24927416
- Data suggest that Firmicutes and Bacteroidetes potentially mediate insulin resistance through modulation of glucagon-like peptide 1 (GLP-1) secretion in obesity. PMID: 25713030
- Data suggest that glucagon-like peptide-1 (GLP-1) may be involved in normal sweet taste signal transmission. PMID: 25678625
- NUCB2/nesfatin-1 is co-localized with GLP-1 and GIP in small intestinal cells. Data support the hypothesis that nesfatin-1 is present in enteroendocrine cells and that it stimulates incretin secretion. PMID: 25930999
- results suggest that the fine-turning of GLP-1 secretion from enteroendocrine L cells is established by the balance between alpha1-, alpha2-, and beta-ARs activation PMID: 25843795
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亞細胞定位:Secreted.; [Glucagon-like peptide 1]: Secreted.
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蛋白家族:Glucagon family
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組織特異性:[Glucagon]: Secreted in the A cells of the islets of Langerhans.; [Glucagon-like peptide 1]: Secreted in the A cells of the islets of Langerhans. Secreted from enteroendocrine L cells throughout the gastrointestinal tract. Also secreted in selected neuron
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