Mouse Glucose-dependent insulin-releasing polypeptide,GIP ELISA Kit
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中文名稱:小鼠葡萄糖依賴性胰島素釋放多肽(GIP)酶聯(lián)免疫試劑盒
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貨號(hào):CSB-E08486m
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規(guī)格:96T/48T
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價(jià)格:¥3200/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:小鼠葡萄糖依賴性胰島素釋放多肽(GIP)酶聯(lián)免疫試劑盒(CSB-E08486m)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的GIP含量。GIP(葡萄糖依賴性促胰島素釋放多肽)是一種腸促胰島素激素,參與糖代謝和脂代謝,通過多種途徑增加胰島素敏感性,改善胰島素抵抗。其研究機(jī)制涉及調(diào)節(jié)脂肪細(xì)胞代謝、促進(jìn)β細(xì)胞增殖等,對(duì)治療2型糖尿病和肥胖具有重要意義。試劑盒檢測(cè)范圍為0.156 ng/mL-10 ng/mL,適用于科研領(lǐng)域探索GIP在糖代謝調(diào)控、胰島素分泌及能量平衡中的作用機(jī)制。該產(chǎn)品適用于小鼠模型中GIP與糖尿病、肥胖癥或胃腸道功能相關(guān)的基礎(chǔ)研究,實(shí)驗(yàn)結(jié)果需結(jié)合其他代謝指標(biāo)進(jìn)行綜合分析,嚴(yán)禁用于臨床診斷。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名:GipGastric inhibitory polypeptide ELISA Kit; GIP ELISA Kit; Glucose-dependent insulinotropic polypeptide ELISA Kit
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縮寫:
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Uniprot No.:
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種屬:Mus musculus (Mouse)
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樣本類型:serum, plasma, tissue homogenates
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檢測(cè)范圍:0.156 ng/mL-10 ng/mL
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靈敏度:0.039 ng/mL
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測(cè)波長(zhǎng):450 nm
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研究領(lǐng)域:Signal Transduction
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測(cè)定原理:quantitative
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測(cè)定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse GIP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 88 Range % 84-92 1:2 Average % 97 Range % 94-101 1:4 Average % 102 Range % 97-108 1:8 Average % 94 Range % 89-98 -
回收率:
The recovery of mouse GIP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 102 98-107 EDTA plasma (n=4) 93 82-97 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average Corrected 10 2.134 2.257 2.196 2.080 5 1.675 1.739 1.707 1.591 2.5 1.144 1.185 1.165 1.049 1.25 0.672 0.696 0.684 0.568 0.625 0.404 0.423 0.414 0.298 0.312 0.288 0.293 0.291 0.175 0.156 0.202 0.209 0.206 0.090 0 0.115 0.117 0.116 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- Lacticaseibacillus paracasei HP7 Improves Gastric Emptying by Modulating Digestive Factors in a Loperamide-Induced Functional Dyspepsia Mouse Model JW Jeong, D Lee, H Kim, H Gwon,Journal of Microbiology and Biotechnology,2025
- The role of gut microbiota in anorexia induced by T-2 toxin T Huang,Ecotoxicology and environmental safety,2024
- Antidiabetic Effects of Bisamide Derivative of Dicarboxylic Acid in Metabolic Disorders A Vladimirovna Pakhomova,Int J Mol Sci.,2020
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能:Potent stimulator of insulin secretion and relatively poor inhibitor of gastric acid secretion.
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基因功能參考文獻(xiàn):
- GIP stimulation induces a switch in GIPR recycling from a rapid endosomal to a slow trans-Golgi network (TGN) pathway. GPCR kinases and b-arrestin2 are required for this switch in recycling. PMID: 27974210
- GIP provides a novel link between the immune system and the gut, with proinflammatory-immune modulatory function but minor glucose regulatory relevance in the context of acute endotoxemia. PMID: 27350651
- our data suggest that the metabolic hormone GIP plays an important role in bone marrow hematopoiesis PMID: 28250160
- It was demonstrated that cardiomyocytes represent a direct target of GIP action in vitro, and that GIP ameliorated AngII-induced cardiac hypertrophy via suppression of cardiomyocyte enlargement, apoptosis, and fibrosis in vivo. PMID: 27375170
- The loss of food-induced GIP response in Roux-limb of intestine likely contributes to the attenuated serum GIP response to feeding. PMID: 26266950
- GIP induces the expression of the proatherogenic cytokine osteopontin (OPN) in mouse arteries via local release of endothelin-1 and activation of CREB. GIPR mRNA is higher in symptomatic carotid endarterectomy patients. PMID: 26395740
- These studies support the hypothesis that a reduction in GIP signaling using a GIP-neutralizing mAb might provide a useful method for the treatment and prevention of obesity and related disorders. PMID: 26487006
- This study provides evidences that GIP acts directly on osteoblasts and is capable of improving collagen maturity and fibril diameter. PMID: 25582623
- There was decrease in GIP gene transcripts and protein in the gut of HNF1a-null mice. PMID: 25979074
- Circulating levels of GIP were significantly decreased in FABP5-deficient mice. PMID: 25268051
- phosphatidylinositol 3-kinase gamma has a role in insulin secretion induced by glucose-dependent insulinotropic polypeptide PMID: 25288806
- The results describe key beneficial immunoregulatory properties for glucose-dependent insulinotropic polypeptide in diet-induced obesity and reveal that its augmentation ameliorates adipose tissue inflammation and improves insulin resistance. PMID: 25217161
- GIP-reduced mice demonstrate that partial reduction of GIP does not extensively alter glucose tolerance, but it alleviates obesity and lessens the degree of insulin resistance under high-fat diet conditions, suggesting a potential therapeutic value. PMID: 24584548
- It was concluded from these observations that GIP, but not glucagon-like peptide-1, directly activates PepT1 activity by a cAMP-dependent signaling pathway in jejunum. PMID: 24072682
- A novel acylated form of (d-Ala(2))GIP with improved antidiabetic potential, lacking effect on body fat stores. PMID: 23518200
- The results indicated that ectopic glucose-dependent insulinotropic polypeptide expression in pancreatic beta cells maintains insulin secretion in the absence of proglucagon-derived peptides, revealing a novel mechanism for sustaining incretin action. PMID: 23099862
- Rfx6 increases GIP expression and content in enteroendocrine K-cells and is involved in GIP hypersecretion in high fat diet-induced obesity. PMID: 23192339
- GIP signaling may play a role in early embryonic pancreas differentiation to form insulin-positive cells or beta-cells. PMID: 21270265
- truncated forms of GIP exhibit potent anti-diabetic actions, without pro-obesity effects, and that the C-terminus contributes to the lipogenic actions of GIP PMID: 20231880
- GIP is expressed in and secreted from pancreatic islets and promotes islet glucose competence and also could support islet development and/or survival. PMID: 20138041
- substantial quantities of glycated GIP exist within the intestines of diabetic ob/ob mice, suggesting that this may be a contributing factor to the physiological disarray of this syndrome PMID: 11954659
- a novel pathway of obesity promotion via GIP; GIP directly links overnutrition to obesity PMID: 12068290
- Novel insulin/GIP co-producing cell lines provide unexpected insights into Gut K-cell function in vivo. PMID: 12124779
- Calcium mobilization from intracellular and extracellular sources, independent from K(ATP) channels, regulates secretion from some, but not all, subpopulations of enteroendocrine cells. PMID: 12676650
- GIP is the major insulinotropic factor in the secretion of insulin in response to glucose load in K(ATP) channel-deficient mice. PMID: 15362972
- Transcription factor PDX-1 plays a critical role in the cell-specific expression of the GIP gene. PMID: 15486225
- The physiological importance of glucose-dependent insulinotropic polypeptide has been investigated in single and double incretin receptor knockout mice. PMID: 15780432
- PI3K/PKB/Foxo1 signaling mediates GIP suppression of bax gene expression, which is a key pathway by which GIP regulates beta-cell apoptosis in vivo PMID: 15817464
- GIP plays a crucial role in switching from fat oxidation to fat accumulation under the diminished insulin action as a potential target for secondary prevention of insulin resistance PMID: 16105663
- Because GIPR(-/-) mice exhibited an increased plasma calcium concentration after meal ingestion, GIP directly links calcium contained in meal to calcium deposition on bone. PMID: 16469773
- Prohormone convertase 1/3 is essential for processing of the glucose-dependent insulinotropic polypeptide precursor PMID: 16476726
- protein kinase B, LKB1, and AMP-activated protein kinase have roles in activation of lipoprotein lipase by glucose-dependent insulinotropic polypeptide in adipocytes PMID: 17244606
- Glucose-dependent insulinotropic peptide inhibits bone resorption and stimulates bone formation PMID: 17321229
- First characterization of the anatomical distribution of GIP-immunoreactive cells in the rat brain providing an anatomical framework for future investigations regarding the functions of GIP in the central nervous system. PMID: 17510976
- Administration of resistin to adipocytes mimicked the effects of GIP on the PKB/LKB1/AMPK/LPL pathway: increasing phosphorylation of PKB, reducing levels of phosphorylated LKB1 and AMPK, and increasing LPL activity. PMID: 17890220
- genetic inactivation of GIP signaling can prevent the development of aging-associated insulin resistance through body composition changes PMID: 17937928
- GATA-4 may function to augment or enhance GIP expression rather than act as an initiator of GIP transcription. PMID: 18343025
- Gpr40 modulates FFA-stimulated insulin secretion from beta-cells not only directly but also indirectly via regulation of incretin(GIP and GLP-1) secretion PMID: 18519800
- The GIP-induced increase in glucose transport appears to be mediated, at least in part, by SGLT-1. PMID: 18719661
- These results indicate that inhibition of GIP signaling increases adiponectin levels, resulting in increased fat oxidation in peripheral tissues under high-fat diet. PMID: 18723001
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亞細(xì)胞定位:Secreted.
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蛋白家族:Glucagon family
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