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  4. Mouse Glypican-3(GPC-3)ELISA Kit

Mouse Glypican-3(GPC-3)ELISA Kit

  • 中文名稱：
    小鼠磷脂酰肌醇蛋白聚糖3(GPC-3)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E15877m
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠磷脂酰肌醇蛋白聚糖3(GPC-3)酶聯(lián)免疫試劑盒（CSB-E15877m）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液樣本中的GPC3含量。GPC3是一種糖基磷脂酰肌醇錨定蛋白，在正常肝臟組織中幾乎不表達(dá)，在肝癌等多種腫瘤中高表達(dá)。研究機(jī)制上，其可促進(jìn)腫瘤細(xì)胞增殖、遷移等。因其在腫瘤組織中的特異性，成為極具潛力的腫瘤治療靶點(diǎn)，為肝癌等治療提供了新思路。試劑盒檢測(cè)范圍為3.12 ng/mL-200 ng/mL，適用于小鼠模型中的GPC-3表達(dá)水平監(jiān)測(cè)、腫瘤相關(guān)信號(hào)通路研究以及體外藥物干預(yù)效果評(píng)估等科研場(chǎng)景，為肝臟疾病機(jī)制研究及生物標(biāo)志物篩選提供高效工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
  • 別名：
    Gpc3 ELISA Kit; Glypican-3 [Cleaved into: Glypican-3 alpha subunit; Glypican-3 beta subunit] ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, cell culture supernates
  • 檢測(cè)范圍：
    3.12 ng/mL-200 ng/mL
  • 靈敏度：
    0.78 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse GPC-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 92
    Range % 84-95
    1:2 Average % 94
    Range % 84-98
    1:4 Average % 94
    Range % 85-97
    1:8 Average % 90
    Range % 84-94
  • 回收率：
    The recovery of mouse GPC-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 99 91-103
    EDTA plasma (n=4) 95 90-99
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    200 2.173 2.202 2.188 2.050
    100 1.648 1.683 1.666 1.528
    50 0.936 0.911 0.924 0.786
    25 0.549 0.540 0.545 0.407
    12.5 0.301 0.323 0.312 0.174
    6.25 0.245 0.251 0.248 0.110
    3.12 0.178 0.181 0.180 0.042
    0 0.137 0.139 0.138 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Cell surface proteoglycan that bears heparan sulfate. Negatively regulates the hedgehog signaling pathway when attached via the GPI-anchor to the cell surface by competing with the hedgehog receptor PTC1 for binding to hedgehog proteins. Binding to the hedgehog protein SHH triggers internalization of the complex by endocytosis and its subsequent lysosomal degradation. Positively regulates the canonical Wnt signaling pathway by binding to the Wnt receptor Frizzled and stimulating the binding of the Frizzled receptor to Wnt ligands. Positively regulates the non-canonical Wnt signaling pathway. Binds to CD81 which decreases the availability of free CD81 for binding to the transcriptional repressor HHEX, resulting in nuclear translocation of HHEX and transcriptional repression. Inhibits the dipeptidyl peptidase activity of DPP4. Plays a role in limb patterning and skeletal development by controlling the cellular response to BMP4. Modulates the effects of growth factors BMP2, BMP7 and FGF7 on renal branching morphogenesis. Required for coronary vascular development. Plays a role in regulating cell movements during gastrulation.
  • 基因功能參考文獻(xiàn)：
    1. Coupling of pGPC3 to liposomes was essential for effective priming of GPC3-specific CTLs. PMID: 26616051
    2. The expression of GPC-3 was altered by DEN treatment. PMID: 24377529
    3. Finding represents a rare four layer genomic overlap consisting of growth associated quantitative trait locus (QTL), body mass associated Gpc3 gene, highly conserved miRNA gene and mature miRNA seed SNP identified in the lean mouse. PMID: 21152117
    4. Hepatocyte overexpression of GPC3 suppresses hepatocyte proliferation and liver regeneration and alters gene expression profiles. PMID: 20812357
    5. glypican-3 is involved in the recruitment of M2-polarized tumor-associated macrophages in hepatocellular carcinoma PMID: 19838081
    6. GPC3 inhibits the PI3K/Akt anti-apoptotic pathway while it stimulates the p38MAPK stress-activated one in murine mammary adenocarcinoma LM3 cells PMID: 19288189
    7. Gpc3 function in development of Simpson-Golabi-Behmel syndrome is IGF-independent PMID: 11846487
    8. GPC3 knockout mice exhibit alterations in the Wnt signaling pathway, which is also associated with the regulation of cell proliferation PMID: 15537637
    9. Polymorphisms in the 3' untranslated region of Gpc3 are strong candidates for the causal sequence variation and loss-of-function mutations can cause overgrowth and developmental abnormalities. PMID: 15799711
    10. Altered hematopoiesis in glypican-3-deficient mice results in decreased osteoclast differentiation and a delay in endochondral ossification. PMID: 15936336
    11. Zhx2 acts as a repressor of Gpc3 in the adult liver. Gpc3 is activated in the regenerating liver in an Afr2-dependent manner. PMID: 17668883
    12. A novel glycosyl phosphatidyl inositol (GPI)-linked proteoglycan, Glypican3 (GPC3, binding to GLUT4 was identified. PMID: 18343214
    13. Glypican-3 regulates migration, adhesion and actin cytoskeleton organization in mammary tumor cells through Wnt signaling modulation. PMID: 18404367
    14. GPC3 acts as a negative regulator of Hedgehog signaling during mammalian development. PMID: 18477453
    15. The heparan sulfate proteoglycan (HSPG) glypican-3 mediates commitment of MC3T3-E1 cells toward osteogenesis. PMID: 19479939
    16. The Gpc3-null mice show increased levels of Ihh protein and signalling, but similar levels of Ihh messenger RNA. PMID: 19590577
    17. Data show that glypican-3-deficient mice display a high incidence of congenital cardiac malformations like ventricular septal defects, common atrioventricular canal and double outlet right ventricle. PMID: 19733558

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  • 亞細(xì)胞定位：
    Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side.
  • 蛋白家族：
    Glypican family
  • 組織特異性：
    In the developing limb, absent from the apical epidermal ridge at 11 dpc but highly expressed in the underlying mesenchyme. Expression in the mesenchyme at this stage is asymmetric with highest levels in the regions of the distal mesenchyme within the pro
  • 數(shù)據(jù)庫(kù)鏈接：