搡老女人多毛老妇女中国,日韩亚洲欧美中文高清在线,人妻少妇一区二区三区,色妞色综合久久夜夜,日本熟妇xxxx

Your Good Partner in Biology Research

  1. 首頁
  2. 試劑盒
  3. 酶聯(lián)免疫試劑盒
  4. Mouse Hepatocyte Growth Factor Receptor(C-MET/HGFR)ELISA Kit

Mouse Hepatocyte Growth Factor Receptor(C-MET/HGFR)ELISA Kit

  • 中文名稱：
    小鼠肝細(xì)胞生長因子受體(c-MET/HGFR)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E13492m
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠肝細(xì)胞生長因子受體(c-MET/HGFR)酶聯(lián)免疫試劑盒（CSB-E13492m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的MET含量。MET是一種原癌基因編碼的受體酪氨酸激酶。其異常激活與腫瘤的發(fā)生、發(fā)展、轉(zhuǎn)移及預(yù)后密切相關(guān)。研究機(jī)制主要聚焦于MET信號通路，阻斷該通路可抑制腫瘤細(xì)胞增殖、存活和遷移，為腫瘤治療提供了潛在的靶點(diǎn)策略。試劑盒檢測范圍為0.078 ng/mL-5 ng/mL，靈敏度為0.02 ng/mL。適用于小鼠疾病模型中腫瘤微環(huán)境研究、肝臟再生機(jī)制探索以及靶向藥物干預(yù)實(shí)驗(yàn)的療效評估。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Met ELISA Kit; Hepatocyte growth factor receptor ELISA Kit; HGF receptor ELISA Kit; EC 2.7.10.1 ELISA Kit; HGF/SF receptor ELISA Kit; Proto-oncogene c-Met ELISA Kit; Scatter factor receptor ELISA Kit; SF receptor ELISA Kit; Tyrosine-protein kinase Met ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.078 ng/mL-5 ng/mL
  • 靈敏度：
    0.02 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cancer
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

    　

    Intra-Assay Precision

    Inter-Assay Precision

    Sample

    1

    2

    3

    1

    2

    3

    n

    20

    20

    20

    20

    20

    20

    Mean(pg/ml)

    639.988

    613.434

    633.318

    588.302

    625.563

    621.144

    SD

    0.035

    0.040

    0.036

    0.045

    0.048

    0.052

    CV(%)

    4.698

    5.548

    4.871

    6.447

    6.557

    7.143

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse C-MET/HGFR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    91

    Range %

    87-96

    1:2

    Average %

    102

    Range %

    96-107

    1:4

    Average %

    91

    Range %

    87-96

    1:8

    Average %

    95

    Range %

    89-99

  • 回收率：

    The recovery of mouse C-MET/HGFR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    97

    92-101

    EDTA plasma (n=4)

    104

    92-108

  • 標(biāo)準(zhǔn)曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    Corrected

    5

    2.723

    2.854

    2.789

    2.701

    2.5

    1.856

    1.987

    1.922

    1.834

    1.25

    1.219

    1.264

    1.242

    1.154

    0.625

    0.715

    0.732

    0.724

    0.636

    0.312

    0.399

    0.386

    0.393

    0.305

    0.156

    0.248

    0.255

    0.252

    0.164

    0.078

    0.158

    0.162

    0.160

    0.072

    0

    0.087

    0.088

    0.088

    　

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. The RAS-ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling plays a role in gastrulation, development and migration of muscles and neuronal precursors, angiogenesis and kidney formation. In adults, participates in wound healing as well as organ regeneration and tissue remodeling. Promotes also differentiation and proliferation of hematopoietic cells. May regulate cortical bone osteogenesis.; (Microbial infection) Acts as a receptor for Listeria monocytogenes internalin InlB, mediating entry of the pathogen into cells.
  • 相關(guān)疾病：
    Activation of Met after rearrangement with the TPR (translocated promoter) locus of chromosome 1 produces an oncogenic protein.
  • 亞細(xì)胞定位：
    Membrane; Single-pass type I membrane protein.
  • 蛋白家族：
    Protein kinase superfamily, Tyr protein kinase family
  • 數(shù)據(jù)庫鏈接：