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  4. Mouse Hepatocyte growth factor activator(HGFAC) ELISA kit

Mouse Hepatocyte growth factor activator(HGFAC) ELISA kit

  • 中文名稱：
    小鼠肝細胞生長因子激活物(HGFAC)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL010328MO
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠肝細胞生長因子激活物(HGFAC)酶聯(lián)免疫試劑盒（CSB-EL010328MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的HGFAC含量。HGFAC（肝細胞生長因子激活因子）是一種關(guān)鍵的酶，通過催化肝細胞生長因子（HGF）的激活，將其轉(zhuǎn)化為具有生物活性的異二聚體，參與細胞增殖、遷移和存活等過程。其研究背景涉及細胞信號傳導和疾病發(fā)生，機制上與HGF的激活和細胞功能密切相關(guān)。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，適用于體外研究HGFAC在肝臟疾病模型、腫瘤發(fā)生機制或組織修復相關(guān)實驗中的表達動態(tài)，為探索HGF/HGFAC信號通路在細胞生物學及分子機制研究提供可靠工具，滿足基礎(chǔ)科研中對蛋白定量分析的需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Hgfac ELISA Kit; Hepatocyte growth factor activator ELISA Kit; HGF activator ELISA Kit; HGFA ELISA Kit; EC 3.4.21.-) [Cleaved into: Hepatocyte growth factor activator short chain; Hepatocyte growth factor activator long chain] ELISA Kit
  • 縮寫：
    HGFAC
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    31.25 pg/mL-2000 pg/mL
  • 靈敏度：
    7.8 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse HGFAC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 104
    Range % 98-109
    1:2 Average % 97
    Range % 90-101
    1:4 Average % 92
    Range % 86-95
    1:8 Average % 97
    Range % 93-102
  • 回收率：
    The recovery of mouse HGFAC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 94 87-98
    EDTA plasma (n=4) 98 92-103
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    2000 2.580 2.469 2.525 2.355
    1000 1.826 1.902 1.864 1.694
    500 1.203 1.183 1.193 1.023
    250 0.682 0.664 0.673 0.503
    125 0.399 0.385 0.392 0.222
    62.5 0.277 0.284 0.281 0.111
    31.25 0.203 0.209 0.206 0.036
    0 0.169 0.171 0.170 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Activates hepatocyte growth factor (HGF) by converting it from a single chain to a heterodimeric form.
  • 基因功能參考文獻：
    1. HGFA is required for repair of injured intestinal mucosa but is not essential for normal development during embryogenesis or after birth. PMID: 15521012
  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Peptidase S1 family
  • 數(shù)據(jù)庫鏈接：