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  4. Mouse High density lipoprotein cholesterol, HDL-C ELISA kit

Mouse High density lipoprotein cholesterol, HDL-C ELISA kit

  • 中文名稱：
    小鼠高密度脂蛋白膽固醇檢測(cè)試劑盒
  • 貨號(hào)：
    CSB-E12874m
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠高密度脂蛋白膽固醇檢測(cè)試劑盒（CSB-E12874m）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的HDL-C含量。HDL-C（高密度脂蛋白膽固醇）是一種被稱為“好”的膽固醇，有助于清除血液中的多余膽固醇，降低心臟病風(fēng)險(xiǎn)。研究顯示，高HDL-C水平與較低的冠心病風(fēng)險(xiǎn)相關(guān)，其作用機(jī)制包括促進(jìn)膽固醇逆向轉(zhuǎn)運(yùn)和抗氧化等。試劑盒檢測(cè)范圍為7.8 ng/mL-500 ng/mL，適用于心血管疾病模型、肥胖或糖尿病研究中脂代謝動(dòng)態(tài)監(jiān)測(cè)、藥物干預(yù)效果評(píng)估等科研場(chǎng)景。通過檢測(cè)血清、血漿樣本可系統(tǒng)性評(píng)估循環(huán)系統(tǒng)中HDL-C水平，而組織勻漿檢測(cè)則有助于探索特定器官（如肝臟、脂肪組織）的局部脂質(zhì)代謝調(diào)控機(jī)制，為代謝性疾病基礎(chǔ)研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
    HDL-C
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    7.8 ng/mL-500 ng/mL
  • 靈敏度：
    1.95 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Others
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse HDL-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1000Average %88
    Range %84-92
    1:2000Average %91
    Range %85-97
    1:4000Average %99
    Range %95-104
    1:8000Average %92
    Range %88-96
  • 回收率：
    The recovery of mouse HDL-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9690-102
    EDTA plasma (n=4)9791-104
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    5002.509 2.598 2.554 2.440
    2502.045 2.068 2.057 1.943
    1251.401 1.457 1.429 1.315
    62.50.977 0.999 0.988 0.874
    31.20.572 0.598 0.585 0.471
    15.60.315 0.338 0.327 0.213
    7.80.256 0.267 0.262 0.148
    00.113 0.115 0.114 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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