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  4. Mouse Histidine-rich glycoprotein(HRG) ELISA kit

Mouse Histidine-rich glycoprotein(HRG) ELISA kit

  • 中文名稱：
    小鼠富組氨酸糖蛋白(HRG)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL010736MO
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠富組氨酸糖蛋白(HRG)酶聯(lián)免疫試劑盒（CSB-EL010736MO）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的HRG含量。小鼠富組氨酸糖蛋白（HRG）是一種位于血漿和血小板中的糖蛋白，其生理功能尚不明確，但已知可結(jié)合血紅素、染料和二價金屬離子，并可能具有抗菌活性。HRG在凝血過程中可能通過抑制凝血因子FXIIa的活性來調(diào)節(jié)血栓形成，其功能受蛋白二硫鍵異構(gòu)酶PDI的調(diào)控。試劑盒檢測范圍為6.25 ng/mL-400 ng/mL，其在小鼠模型中的表達水平變化常被用于研究感染性疾病、腫瘤微環(huán)境調(diào)控及代謝紊亂等科研領(lǐng)域；為探索HRG在凝血功能異常、炎癥反應(yīng)或免疫相關(guān)疾病模型中的分子機制提供可靠工具，特別適用于基礎(chǔ)醫(yī)學(xué)研究、藥效評價及分子病理學(xué)等科研場景，助力研究者深入解析HRG在疾病發(fā)生發(fā)展中的生物學(xué)功能。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    HrgHistidine-rich glycoprotein ELISA Kit; Histidine-proline-rich glycoprotein ELISA Kit; HPRG ELISA Kit
  • 縮寫：
    HRG
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    6.25 ng/mL-400 ng/mL
  • 靈敏度：
    3.12 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse HRG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1000Average %87
    Range %81-96
    1:2000Average %89
    Range %83-97
    1:4000Average %97
    Range %92-103
    1:8000Average %95
    Range %88-98
  • 回收率：
    The recovery of mouse HRG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9489-100
    EDTA plasma (n=4)9590-99
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2Average
    4000.144 0.146 0.145
    2000.226 0.232 0.229
    1000.371 0.377 0.374
    500.539 0.564 0.552
    250.965 0.992 0.979
    12.51.704 1.563 1.634
    6.252.341 2.359 2.350
    02.761 2.750 2.756
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Plasma glycoprotein that binds a number of ligands such as heme, heparin, heparan sulfate, thrombospondin, plasminogen, and divalent metal ions. Binds heparin and heparin/glycosaminoglycans in a zinc-dependent manner. Binds heparan sulfate on the surface of liver, lung, kidney and heart endothelial cells. Binds to N-sulfated polysaccharide chains on the surface of liver endothelial cells. Inhibits rosette formation. Acts as an adapter protein and is implicated in regulating many processes such as immune complex and pathogen clearance, cell chemotaxis, cell adhesion, angiogenesis, coagulation and fibrinolysis. Mediates clearance of necrotic cells through enhancing the phagocytosis of necrotic cells in a heparan sulfate-dependent pathway. This process can be regulated by the presence of certain HRG ligands such as heparin and zinc ions. Binds to IgG subclasses of immunoglobins containing kappa and lambda light chains with different affinities regulating their clearance and inhibiting the formation of insoluble immune complexes. Tethers plasminogen to the cell surface. Binds T-cells and alters the cell morphology. Acts as a regulator of the vascular endothelial growth factor (VEGF) signaling pathway; inhibits endothelial cell motility by reducing VEGF-induced complex formation between PXN/paxillin and ILK/integrin-linked protein kinase and by promoting inhibition of VEGF-induced tyrosine phosphorylation of focal adhesion kinases and alpha-actinins in endothelial cells. Also plays a role in the regulation of tumor angiogenesis and tumor immune surveillance. Normalizes tumor vessels and promotes antitumor immunity by polarizing tumor-associated macrophages, leading to decreased tumor growth and metastasis. Modulates angiogenesis by blocking the CD6-mediated antiangiongenic effect of thrombospondins, THBS1 and THBS2.
  • 基因功能參考文獻：
    1. HRG binds to alpha2 integrin through low-affinity interactions in a heparin sulfate-independent manner, thereby blocking endothelial cells adhesion to collagen I. PMID: 26051322
    2. Mice deficient in HRG have normal hemostasis, but demonstrate accelerated thrombosis via the contact system. PMID: 25691157
    3. mononuclear phagocytes have specific binding sites for HRG and that these cells are essential for uptake of HRG from blood and distribution of HRG in tissues. Thus, inflammatory cells mediate the effect of HRG on tumor growth and metastatic spread. PMID: 25243896
    4. Results show that HRG is a novel transcriptional target gene of FXR in human hepatoma cells, human upcyteVR primary hepatocytes and 3D human liver microtissues in vitro and in mouse liver in vivo. PMID: 25363753
    5. HRG-deficiency promotes tumor progression via enhanced platelet activity. HRG is a potent tumor suppressor, with capacity to attenuate the angiogenic switch, tumor growth, EMT and subsequent metastatic spread, by regulating platelet activity. PMID: 23793459
    6. CD36-mediated anti-angiogenic activity has context-dependent role in the tumor microenvironment in the presence of TSP-1 while HRG modulates this activity. PMID: 22808089
    7. HRG loss influences macrophage gene regulation, leading to excessive stimulation of tumor angiogenesis, suppression of tumor immune response, and increased tumor growth and metastatic spread PMID: 22374984
    8. the identification and characterization of a novel peptide, VTIP-peptide (VTIP-P), which binds and enhances the activation of CREM/CREB PMID: 19968777
    9. role for HRG as a regulator of inflammation and in the defense at the local site of bacterial infection PMID: 20587784
    10. HRG acts as a soluble adaptor molecule that binds to cells at sites of tissue injury, tumor growth, and angiogenesis PMID: 15220341
    11. CD36 mediates the antiangiogenic effect of thrombospondin-2, which is modulated by histidine-rich glycoprotein PMID: 15748999
    12. HRG plays a role as both an anticoagulant and an antifibrinolytic modifier, and may regulate platelet function in vivo PMID: 15869579
    13. antifungal role of HRG in innate immunity PMID: 18797515

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  • 亞細(xì)胞定位：
    Secreted.
  • 組織特異性：
    Expressed in liver, blood plasma, serum and in platelets. Also present in fibrin clots, wound fluid from acute wounds and chronic leg ulcers.
  • 數(shù)據(jù)庫鏈接：