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  4. Mouse Hydroxyacid oxidase 1(HAO1) ELISA kit

Mouse Hydroxyacid oxidase 1(HAO1) ELISA kit

  • 中文名稱：
    小鼠羥基酸氧化酶1(HAO1)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL010127MO
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠羥基酸氧化酶1(HAO1)酶聯(lián)免疫試劑盒（CSB-EL010127MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的HAO1含量。小鼠羥基酸氧化酶1（HAO1）是草酸代謝中的關(guān)鍵酶，其表達(dá)下調(diào)可顯著降低細(xì)胞草酸生成，具有作為干預(yù)細(xì)胞草酸生成靶點(diǎn)的潛力。研究機(jī)制涉及HAO1在氧化應(yīng)激損傷中的作用，以及其對相關(guān)疾病如糖尿病和腫瘤轉(zhuǎn)移的影響。試劑盒檢測范圍為6.25 pg/mL-400 pg/mL，適用于探索HAO1在代謝通路中的分子機(jī)制、評(píng)估基因敲除/過表達(dá)模型小鼠的酶活性變化，或研究藥物干預(yù)、營養(yǎng)狀態(tài)等因素對HAO1表達(dá)的影響，為脂質(zhì)代謝相關(guān)基礎(chǔ)研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Hao1 ELISA Kit; Gox1 ELISA Kit; Hao-1 ELISA Kit; Hydroxyacid oxidase 1 ELISA Kit; HAOX1 ELISA Kit; EC 1.1.3.15 ELISA Kit; Glycolate oxidase ELISA Kit; GOX ELISA Kit
  • 縮寫：
    HAO1
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    6.25 pg/mL-400 pg/mL
  • 靈敏度：
    1.56 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse HAO1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %94
    Range %83-107
    1:2Average %95
    Range %89-103
    1:4Average %92
    Range %85-105
    1:8Average %91
    Range %87-99
  • 回收率：
    The recovery of mouse HAO1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9892-103
    EDTA plasma (n=4)9690-104
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    4002.413 2.347 2.380 2.198
    2001.603 1.589 1.596 1.414
    1000.902 0.865 0.884 0.702
    500.549 0.527 0.538 0.356
    250.388 0.363 0.376 0.194
    12.50.282 0.272 0.277 0.095
    6.250.253 0.237 0.245 0.063
    00.180 0.183 0.182
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Has 2-hydroxyacid oxidase activity. Most active on the 2-carbon substrate glycolate, but is also active on 2-hydroxy fatty acids, with high activity towards 2-hydroxy palmitate and 2-hydroxy octanoate.
  • 亞細(xì)胞定位：
    Peroxisome.
  • 蛋白家族：
    FMN-dependent alpha-hydroxy acid dehydrogenase family
  • 組織特異性：
    Liver.
  • 數(shù)據(jù)庫鏈接：