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  4. Mouse Insulin-like growth factor binding protein 4,IGFBP-4 ELISA kit

Mouse Insulin-like growth factor binding protein 4,IGFBP-4 ELISA kit

  • 中文名稱：
    小鼠胰島素樣生長因子結(jié)合蛋白4(IGFBP-4)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E07355m
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3200/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠胰島素樣生長因子結(jié)合蛋白4(IGFBP-4)酶聯(lián)免疫試劑盒（CSB-E07355m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的IGFBP4含量。IGFBP4是一種胰島素樣生長因子結(jié)合蛋白。它在細胞生長、增殖、分化等過程中發(fā)揮重要作用。研究發(fā)現(xiàn)其能與胰島素樣生長因子結(jié)合，調(diào)節(jié)其活性和功能，影響腫瘤細胞生長、侵襲等。目前在腫瘤等疾病的發(fā)生發(fā)展機制及治療研究上受關(guān)注。試劑盒檢測范圍為0.78 ng/mL-50 ng/mL，本試劑盒適用于科研場景中動物模型或體外實驗的樣本分析，例如探究代謝性疾病機制、生長發(fā)育調(diào)控或內(nèi)分泌相關(guān)研究，能夠為小鼠血清或血漿樣本的IGFBP-4動態(tài)變化提供可靠數(shù)據(jù)支持本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Igfbp4 ELISA Kit; Igfbp-4 ELISA Kit; Insulin-like growth factor-binding protein 4 ELISA Kit; IBP-4 ELISA Kit; IGF-binding protein 4 ELISA Kit; IGFBP-4 ELISA Kit
  • 縮寫：
    IGFBP4
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma
  • 檢測范圍：
    0.78 ng/mL-50 ng/mL
  • 靈敏度：
    0.195 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse IGFBP-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 92    
    Range % 88-96    
    1:2 Average % 84    
    Range % 80-88    
    1:4 Average % 97    
    Range % 92-101    
    1:8 Average % 105    
    Range % 100-112    
  • 回收率：
    The recovery of mouse IGFBP-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 95 90-100    
    EDTA plasma (n=4) 88 84-92    
                   
                   
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    ng/ml OD1 OD2 Average Corrected    
    50 2.384 2.495 2.440 2.290    
    25 2.041 2.103 2.072 1.922    
    12.5 1.605 1.569 1.587 1.437    
    6.25 1.002 0.981 0.992 0.842    
    3.12 0.519 0.538 0.529 0.379    
    1.56 0.339 0.352 0.346 0.196    
    0.78 0.263 0.274 0.269 0.119    
    0 0.152 0.147 0.150 　    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
  • 基因功能參考文獻：
    1. Igfbp4 is required for inguinal fat expansion in female mice but not in male mice. However, gonadal WAT expansion, which is prevented by estrogen during high-fat diet, does not require Igfbp4. PMID: 28938423
    2. IGFBP-4 modulates the skeleton in a gender-specific manner, acting as both a cell autonomous and cell non-autonomous factor. PMID: 28184001
    3. exerts a negative effect on basal intestinal growth but plays a positive regulatory role in the intestinotropic actions of GLP-2 PMID: 25514089
    4. IGF-1/IGFbp4 signaling regulated the post-developmental adipose tissue expansion. PMID: 24778188
    5. SOX9 is a transcriptional regulator of IGFBP-4 and that SOX9-induced activation of IGFBP-4 may be one of the mechanisms by which SOX9 suppresses cell proliferation and progression of colon cancer. PMID: 23660500
    6. findings indicate that the defect in p53 function and the increased proteolysis of IGFBP-4, we had observed, represent two components of the same pathway, which contributes to the oncogenic function of Skp2B PMID: 21829624
    7. Inactivation of IGFBP4 diminished the anabolic effects of PAPP-A on bone. PMID: 20144555
    8. Targeted expression of a protease-resistant IGFBP-4 mutant in smooth muscle of transgenic mice PMID: 11923290
    9. IGFBP-4 is a potential growth inhibitor of lymphoid tissues. PMID: 15072544
    10. This study demonstrates that ES of muscle cells in vitro not only directly modulates the gene expression of contractile proteins but also modulates proteins that are part of the IGF regulatory system, in particular IGFBP-4. PMID: 15584093
    11. Crystallization, heparin and IGF binding, and osteogenic activity of IGFBP4 in its native conformation PMID: 15735305
    12. Overexpression of Hbegf in transgenic mice downregulates Igfbp4. PMID: 16019424
    13. IGFBP-4 may have a role in increasing necrosis and apoptosis, but in decreasing mitosis PMID: 17824980
    14. Results indicate that IGFBP-4 functions as a local reservoir to optimize IGF-II actions needed for normal embryogenesis, and also establish that IGFBP-4 proteolysis is required to activate most, if not all, IGF-II mediated growth-promoting activity. PMID: 18258685
    15. IGFBP-4 is an inhibitor of the canonical Wnt signalling required for cardiogenesis and provides a molecular link between insulin-like growth factor signalling and Wnt signalling PMID: 18528331
    16. SOD1(G93A) mutant transgenic mice as a model of amyotrophic lateral sclerosis (ALS) and immunohistochemical studies to investigate the changes of insulin-like growth factor-binding protein 4 (IGFBP4) in the central nervous system. PMID: 18823765

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  • 亞細胞定位：
    Secreted.
  • 數(shù)據(jù)庫鏈接：