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  4. Mouse Insulin-like growth factor-binding protein complex acid labile subunit(IGFALS) ELISA kit

Mouse Insulin-like growth factor-binding protein complex acid labile subunit(IGFALS) ELISA kit

  • 中文名稱：
    小鼠胰島素樣生長(zhǎng)因子結(jié)合蛋白復(fù)合物酸不穩(wěn)定亞基(IGFALS)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL011094MO
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠胰島素樣生長(zhǎng)因子結(jié)合蛋白復(fù)合物酸不穩(wěn)定亞基(IGFALS)酶聯(lián)免疫試劑盒（CSB-EL011094MO）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的IGFALS含量。IGFALS即胰島素樣生長(zhǎng)因子結(jié)合蛋白酸不穩(wěn)定亞基，是胰島素樣生長(zhǎng)因子（IGF）軸的關(guān)鍵組分，能與IGF和結(jié)合蛋白形成三元復(fù)合物，延長(zhǎng)IGF半衰期。目前對(duì)其研究主要聚焦于在生長(zhǎng)發(fā)育、代謝及疾病如腫瘤中的作用機(jī)制。試劑盒檢測(cè)范圍為3.12 ng/mL-200 ng/mL，適用于探索小鼠模型中IGFALS在生長(zhǎng)發(fā)育異常、代謝性疾病、內(nèi)分泌調(diào)控及相關(guān)信號(hào)通路中的功能機(jī)制，也可用于評(píng)估藥物或?qū)嶒?yàn)干預(yù)對(duì)IGF系統(tǒng)的影響，為相關(guān)基礎(chǔ)研究提供可靠的定量分析工具，滿足科研場(chǎng)景下對(duì)IGFALS表達(dá)水平的動(dòng)態(tài)監(jiān)測(cè)需求本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Igfals ELISA Kit; Albs ELISA Kit; AlsInsulin-like growth factor-binding protein complex acid labile subunit ELISA Kit; ALS ELISA Kit
  • 縮寫：
    IGFALS
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    3.12 ng/mL-200 ng/mL
  • 靈敏度：
    0.78 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Others
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse IGFALS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:100Average %96
    Range %93-99
    1:200Average %84
    Range %80-87
    1:400Average %91
    Range %87-94
    1:800Average %107
    Range %104-110
  • 回收率：
    The recovery of mouse IGFALS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9490-98
    EDTA plasma (n=4)8581-89
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    2001.985 1.901 1.943 1.838
    1001.543 1.578 1.561 1.456
    501.055 1.064 1.060 0.955
    250.657 0.648 0.653 0.548
    12.50.346 0.352 0.349 0.244
    6.250.248 0.268 0.258 0.153
    3.120.191 0.193 0.192 0.087
    00.106 0.104 0.105
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    May have an important role in regulating the access of circulating IGFs to the tissues.
  • 基因功能參考文獻(xiàn)：
    1. the Igfals gene has a role in skeletal response of male mice to anabolic hormone therapy PMID: 24424061
    2. A modest reduction in post-natal growth in the null ALS mice and in the ALS-deficient patients was observed PMID: 16114275
  • 亞細(xì)胞定位：
    Secreted, extracellular space.
  • 數(shù)據(jù)庫鏈接：

    KEGG: mmu:16005

    STRING: 10090.ENSMUSP00000060169

    UniGene: Mm.3135