Mouse Interleukin 15,IL-15 ELISA Kit
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中文名稱:小鼠白介素15(IL-15)酶聯(lián)免疫試劑盒
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貨號:CSB-E04604m
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規(guī)格:96T/48T
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價格:¥3200/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:小鼠白介素15(IL-15)酶聯(lián)免疫試劑盒(CSB-E04604m)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的IL15含量。IL15是重要的細(xì)胞因子。其背景在于它參與免疫調(diào)節(jié),在機(jī)體抗感染、抗腫瘤等免疫反應(yīng)中有重要作用。研究機(jī)制方面,IL15可激活NK細(xì)胞和CD8?T細(xì)胞活性,增強(qiáng)其殺傷功能,還參與調(diào)節(jié)T細(xì)胞增殖、分化,是免疫治療領(lǐng)域的潛在靶點。試劑盒檢測范圍為0.3125 - 20ng/ml,靈敏度為0.078 ng/ml。適用于免疫學(xué)基礎(chǔ)研究、炎癥反應(yīng)機(jī)制探索及疾病模型構(gòu)建等領(lǐng)域,例如在感染性疾病模型、自身免疫性疾病研究或腫瘤微環(huán)境分析中,量化 IL - 15 的表達(dá)水平變化;支持實驗室常規(guī)檢測需求,滿足細(xì)胞因子動態(tài)監(jiān)測和生物標(biāo)志物篩選等科研場景應(yīng)用本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名:Il15 ELISA Kit; Interleukin-15 ELISA Kit; IL-15 ELISA Kit
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縮寫:
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Uniprot No.:
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種屬:Mus musculus (Mouse)
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樣本類型:serum, plasma, tissue homogenates
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檢測范圍:0.312ng/ml - 20ng/ml
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靈敏度:0.078 ng/ml
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反應(yīng)時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領(lǐng)域:Immunology
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
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線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-15 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
84
Range %
80-92
1:2
Average %
90
Range %
85-96
1:4
Average %
93
Range %
91-105
1:8
Average %
93
Range %
86-98
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回收率:
The recovery of mouse IL-15 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
95
89-98
EDTA plasma (n=4)
88
84-93
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標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml
OD1
OD2
Average
Corrected
20
2.187
2.054
2.120
1.951
10
1.413
1.467
1.440
1.270
5
0.879
0.928
0.904
0.734
2.5
0.589
0.609
0.599
0.429
1.25
0.355
0.341
0.348
0.178
0.625
0.283
0.273
0.278
0.108
0.312
0.222
0.214
0.218
0.048
0
0.175
0.165
0.170
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數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- Interleukin-15 as a myokine: mechanistic insight into its effect on skeletal muscle metabolism Lucien Nadeau.et al,Applied Physiology, Nutrition, and Metabolism,2018
- Discovery and characterization of a novel humanized anti-IL-15 antibody and its relevance for the treatment of refractory celiac disease and eosinophilic esophagitis Alain P.et al,mAbs ,2017
- Combined effect of aerobic interval training and selenium nanoparticles on expression of IL-15 and IL-10/TNF-α ratio in skeletal muscle of 4T1 breast cancer mice with cachexia. Molanouri Shamsi M.et al,Cytokine. ,2016
- Interleukin-15 suppresses hepatitis B virus replication via IFN-ß production in a C57BL/6 mouse model Yin W et al,International Journal of Rheumatic Diseases,2012
相關(guān)產(chǎn)品
相關(guān)問答
I am having a question concerning your murine IL-15 ELISA kit. I would like
to know, if this kit detects both single-peptide IL-15 and IL-15/Ra complex, or only
either of the forms? I would highly appreciate if you could clear this question for me.
since I would need a kit that detects the both forms.
Our kit CSB-E04604m detects total mouse IL-15, including another form or the receptor binding IL-15 form.
靶點詳情
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功能:Cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL-15 requires interaction of IL-15 with components of IL-2R, including IL-2R beta and probably IL-2R gamma but not IL-2R alpha. In neutrophils, stimulates phagocytosis probably by signaling through the IL15 receptor, composed of the subunits IL15RA, IL2RB and IL2RG, which results in kinase SYK activation.
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基因功能參考文獻(xiàn):
- SINTBAD regulates IL-15-induced autophagy and NKT cell survival. PMID: 30022064
- this study shows that IL-15 supports the generation of protective lung-resident memory CD4 T cells PMID: 29186108
- The small intestinal lamina propria innate lymphoid cells subset are not completely IL-7R dependent, but can persist partially through IL-15 signalling. PMID: 28361874
- IL-15, mainly produced by non-hematopoietic cells in lung tissue and being trans-presented, promoted inflationary T cell survival by increasing expression of Bcl-2 PMID: 29652930
- This review provides significant characterization of specific systemic, cellular and molecular alterations caused by IL-15 superagonist treatment as compared to native IL-15 and compares their anti-tumor effectiveness in multiple preclinical studies [Review] PMID: 28888485
- IL-15 trans-presentation by non-CD4 T cells is the primary mechanism via which IL-15 controls CD4 effector T cell differentiation. PMID: 28807496
- the protective effect on metastasis was lost upon patrolling monocyte or NK cell depletion, IL15 neutralization, or IFNgamma ablation. The combined analysis of these approaches allowed us to establish a hierarchy in which patrolling monocytes, making IL15 in response to primary tumors, activate NK cells and IFNg production that then inhibit lung metastasis formation. PMID: 28811289
- an IL-15 isoform lacking exon-6, IL-15DeltaE6, generated by alternative splicing events of activated immune cells, including macrophages and B cells, is reported. PMID: 27166125
- IL-15, but not IL-15Ralpha, is required for the development of spontaneous and virus-induced Type 1 diabetes. PMID: 26853723
- hetIL15 administration improves the outcome of Adoptive cell transfer in lymphoreplete hosts, a finding with significant implications for improving cell-based cancer immunotherapy strategies. PMID: 27986749
- These data demonstrate that mice with an endogenous IL-15 deficiency are susceptible to the development of severe, enhanced Th2-mediated allergic airway disease, which can be regulated by CD8(+) T cells. PMID: 28093832
- IL-15 induces the activation and survival of effector immune cells that are necessary for its antitumoral activity; but, long-term exposure to IL-15 is associated with the development of important side effects mainly mediated by IFN-gamma-producing T-cells PMID: 27356750
- findings indicated that IL-15 plays an important role in preventing leukemia development. PMID: 26876593
- NK cells activated by IL-4 in cooperation with IL-15 exhibit distinctive characteristics with enhanced immunologic cytotoxicity. PMID: 27551096
- Genetic ablation of the IL2Rbeta chain on CD8(+) T cells restrains inhibitory receptor induction, in particular 2B4 and Tim-3; precludes terminal differentiation of highly defective PD-1(hi) effectors; and rescues memory T-cell development and responsiveness to IL-7-dependent signals. Together, we ascribe a previously unexpected role to IL-2 and IL-15 as instigators of CD8(+) T-cell exhaustion during chronic viral infe... PMID: 27573835
- this study reveals the function of IL-15 in astrocyte survival via Akt phosphorylation in response to OGD-induced damage. PMID: 28110119
- Our new compelling findings have established the critical roles of both IL-7 and IL-15 in the maintenance of memory Th17 cells, and the previously undescribed therapeutic advantages of targeting IL-7 and IL-15 further support a promising clinical translation in Th17 cell-mediated autoimmune and inflammatory disorders. PMID: 27899224
- results show that IL-15 is implicated in the high fat diet-induced lipid accumulation and inflammation in the liver, leading to fatty liver disease PMID: 26868085
- homeostasis of IL-15-dependent lymphocyte subsets is also regulated by trans-presentation of IL-15 by non-hematopoietic cells in the tissue environment. PMID: 26778709
- Recombinant IL-15 is a promising agent for cancer immunotherapy both in humans and animals. PMID: 28286036
- Taken together, these data suggest that CCR7 biases memory CD8 T cells toward IL-7-dependent niches over IL-15-dependent niches, which provides insight into the homeostatic regulation of different memory T-cell subsets. PMID: 27385825
- results demonstrate that IL-15 and RANKL induce osteoclastogenesis synergistically, and IL-15 might play a novel and major role in destructive inflammatory bone diseases PMID: 27608420
- This study provides mechanistic insight into how IL-15 controls the generation of memory CD8 T cells and influences their trafficking and ability to take up residence within peripheral tissues. PMID: 28814601
- findings extend understanding of the differential modes of action between IL-2 and IL-15, and highlight how, although sharing the CD122/CD132 receptor, they elicit such different immune actions PMID: 28507024
- IL-15 induced by inflammatory signals in response to lymphodepletion drives lymphocyte responses, as memory CD8 T cells proliferated in an IL-15-dependent manner. PMID: 27183627
- these findings unveil a role for IL-15 as a critical mediator in defense against systemic candidiasis and hold promise for the design of IL-15-based antifungal immunotherapies. PMID: 28636955
- developmental arrest of Natural killer cells results from a lack of IL-15 availability in the microenvironment. PMID: 27837016
- IL-15 impacts butyrate-producing bacteria and lowers butyrate levels in the absence of overt pathology, which represent events that precede and promote intestinal inflammatory diseases. PMID: 27648810
- endogenous IL-15 does not directly augment the pathogenesis of sepsis but enables the development of septic shock by maintaining NK cell numbers and integrity. PMID: 28031340
- this study shows that IL-15-mediated control of the precursor population affects the development of virus-specific CD8+ T-cell memory PMID: 27457412
- this study shows that unleashed trans-presentation of IL-15 on MyD88-deficient fibroblastic reticular cells during viral infection fosters the activation of cytotoxic and non-cytotoxic group 1 innate lymphoid cells and accelerates clearance of the virus PMID: 27798617
- this study shows that synergistic enhancement of adaptive immunity by the combination of epigallo catechin gallate and vitamin A or E is dependent on both IL-15 and its receptor PMID: 27135790
- Under steady-state conditions, KLF2-deficient NK cells alter their expression of homeostatic homing receptors and subsequently undergo apoptosis due to IL-15 starvation. PMID: 27114551
- The induction of IgM and IgA, which can play pivotal roles in mucosal immunity, was promoted in the presence of IL-15. Collectively, the data implicate IL-15 as the master cytokine that induces B-1a cells to mount a mucosal immune response. PMID: 25748019
- The novel mechanism of IL-15 transfer to the surface of antigen-processing DCs may explain the enhanced potency of IL-15:IL-15Ralpha-coated nanoparticles for antigen delivery. PMID: 26719339
- Muscle FNDC5 mRNA expression and irisin release are not IL-15-dependent in mice. PMID: 25920499
- Virtual memory T cells develop and mediate bystander protective immunity in an IL-15-dependent manner. PMID: 27097762
- Impaired delivery of IL-15 to CD4+ T cells in the colon downmodulates Foxp3 expression and enhances RORgammat expression. CD4+ T cells deprived of IL-15 trigger IBD characterized by production of pro-inflammatory cytokines and accumulation of Th1/Th17 cells. PMID: 26964669
- Most (86%) IL15 in serum resides in the free state, with a minor proportion (14%) residing in complex with IL15Ralpha. Results suggest that IL15 is released as a free molecule by an unknown mechanism rather than via cleavage of membrane-bound IL15/IL15Ralpha. PMID: 26812159
- IL-15 may influence mononuclear phagocytes subsets in the gut in a novel way that alters the frequency of lamina propria Th17 cells. PMID: 26600079
- Downregulation of STAT3 phosphorylation enhances tumoricidal effect of IL-15-activated dendritic cell against doxorubicin-resistant lymphoma and leukemia via TNF-alpha. PMID: 26255115
- Exercise-stimulated interleukin-15 is controlled by AMPK and regulates skin metabolism and aging. PMID: 25902870
- IL-15 specifically down-regulates RARB expression, and RARB may play a protective role in lung injury caused by smoking or viral infections. PMID: 25923039
- Hair follicle expression of IL-15 was required for CD8+ skin-resident memory T (TRM) cells to exert tropism for the epidermis. PMID: 26479922
- High levels of IL-15 can promote tumor destruction and reduce metastasis in breast cancer via effects on NK1.1 positive cells. PMID: 25879689
- The role of the IL-15 receptor alpha in metabolism and obesity is reported. PMID: 26269523
- We demonstrated that IL-15 has a role in mammary cancer immunosurveillance and that IL-15-regulated NK and CD8+ memory cells play a role in long-lasting immunoprevention PMID: 25997501
- Type I interferon signaling is required for generating soluble IL-15 complexes. PMID: 25756182
- data reveal that inflammatory IL-15 promotes optimal responses by memory CD8+ T cells. PMID: 26241055
- IL-15 is involved in adipose tissue regulation and linked to altered mitochondrial function PMID: 25517731
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亞細(xì)胞定位:Secreted.
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蛋白家族:IL-15/IL-21 family
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