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  4. Mouse Keratin, type I cytoskeletal 18 fragment (K18-Asp396）ELISA Kit

Mouse Keratin, type I cytoskeletal 18 fragment (K18-Asp396）ELISA Kit

  • 中文名稱：
    小鼠細胞角蛋白18片段Asp396(K18-Asp396)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E14265m
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠細胞角蛋白18片段Asp396(K18-Asp396)酶聯(lián)免疫試劑盒（CSB-E14265m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CK 18-M30含量。CK 18-M30是細胞角蛋白18的一個片段。它與細胞凋亡相關(guān)，在細胞凋亡時，特定的酶會切割CK18產(chǎn)生M30。在醫(yī)學研究中，可作為細胞凋亡的生物標志物，用于多種疾病如肝臟疾病、腫瘤等的診斷、病情監(jiān)測和預(yù)后評估。試劑盒檢測范圍為15.6 μIU/mL-1000 μIU/mL，該產(chǎn)品可廣泛應(yīng)用于科研領(lǐng)域，例如評估藥物或毒素誘導的肝細胞凋亡模型、上皮源性腫瘤細胞死亡機制研究，或炎癥性疾病中上皮屏障功能損傷的定量分析，為體外實驗提供穩(wěn)定的檢測工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
    CK 18-M30
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    15.6 μIU/mL-1000 μIU/mL
  • 靈敏度：
    3.9 μIU/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse CK 18-M30 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:100 Average % 96  
    Range % 89-104  
    1:200 Average % 95  
    Range % 90-100  
    1:400 Average % 92  
    Range % 87-101  
    1:800 Average % 93  
    Range % 90-97  
  • 回收率：
    The recovery of mouse CK 18-M30 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 89-99  
    EDTA plasma (n=4) 96 90-104  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    µIU/ml OD1 OD2 Average Corrected  
    1000 2.317 2.217 2.267 2.164  
    500 1.467 1.367 1.417 1.314  
    250 0.797 0.777 0.787 0.684  
    125 0.412 0.392 0.402 0.299  
    62.5 0.268 0.258 0.263 0.160  
    31.2 0.199 0.189 0.194 0.091  
    15.6 0.165 0.164 0.165 0.062  
    0 0.103 0.102 0.103 　  
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價