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  4. Mouse Laminin,LN ELISA kit

Mouse Laminin,LN ELISA kit

  • 中文名稱：
    小鼠層連蛋白/板層素(LN)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E04645m
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠層連蛋白/板層素(LN)酶聯(lián)免疫試劑盒（CSB-E04645m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的LN含量。小鼠層連蛋白（LN）是一種細胞外基質(zhì)蛋白，參與細胞粘附、遷移和細胞信號傳導。其功能背景涉及多種生理過程，如胚胎發(fā)育、組織修復和腫瘤生長。研究機制主要聚焦于LN與細胞表面受體相互作用，以及其在細胞骨架形成和細胞間通訊中的作用。試劑盒檢測范圍為1.56 ng/mL-100 ng/mL，該產(chǎn)品適用于基礎科研領域，如探索組織再生機制、纖維化病理模型（如肝/肺纖維化）的分子標志物動態(tài)變化，或體外培養(yǎng)細胞分泌LN的功能研究，為相關疾病機制解析及藥物干預效果評估提供可靠工具。試劑盒支持多種生物樣本類型，滿足不同實驗場景需求，且操作流程簡便，重復性良好，適用于實驗室常規(guī)檢測與高通量篩選。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    N/A
  • 縮寫：
    LN
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    1.56 ng/mL-100 ng/mL
  • 靈敏度：
    0.39 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse LN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %99
    Range %94-102
    1:2Average %87
    Range %84-90
    1:4Average %91
    Range %88-96
    1:8Average %101
    Range %97-105
  • 回收率：
    The recovery of mouse LN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9490-98
    EDTA plasma (n=4)9894-102
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    1002.407 2.469 2.438 2.281
    501.472 1.502 1.487 1.330
    250.909 0.935 0.922 0.765
    12.50.597 0.578 0.588 0.431
    6.250.364 0.378 0.371 0.214
    3.120.274 0.298 0.286 0.129
    1.560.209 0.211 0.210 0.053
    00.158 0.156 0.157
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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