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  4. Mouse Macrophage-Derived Chemokine,MDC ELISA kit

Mouse Macrophage-Derived Chemokine,MDC ELISA kit

  • 中文名稱：
    小鼠巨噬細胞來源的趨化因子(MDC/CCL22)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E04661m
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3200/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠巨噬細胞來源的趨化因子(MDC/CCL22)酶聯(lián)免疫試劑盒（CSB-E04661m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、組織培養(yǎng)上清液、細胞裂解物樣本中的CCL22含量。CCL22是一種趨化因子。它在免疫調(diào)節(jié)等生理過程中發(fā)揮重要作用。在研究機制上，其主要通過與特定受體結(jié)合，引導(dǎo)免疫細胞遷移和激活。目前圍繞CCL22在腫瘤免疫、自身免疫病等領(lǐng)域有諸多研究，探索其在疾病發(fā)生發(fā)展中的作用。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，靈敏度為23.672 pg/mL。本產(chǎn)品可廣泛應(yīng)用于免疫學、炎癥機制研究及藥物開發(fā)等科研領(lǐng)域，幫助研究者分析 MDC/CCL22 在不同實驗?zāi)Ｐ停ㄈ缦⒆陨砻庖呒膊』蚰[瘤免疫調(diào)控）中的表達變化，為揭示趨化因子介導(dǎo)的細胞遷移與信號通路提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Ccl22 ELISA Kit; Abcd1 ELISA Kit; Scya22C-C motif chemokine 22 ELISA Kit; Activated B and dendritic cell-derived ELISA Kit; CC chemokine ABCD-1 ELISA Kit; Small-inducible cytokine A22 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell culture supernates, cell lysates
  • 檢測范圍：
    31.25 pg/mL-2000 pg/mL
  • 靈敏度：
    23.672 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse MDC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 89    
    Range % 83-92    
    1:2 Average % 100    
    Range % 92-103    
    1:4 Average % 94    
    Range % 81-98    
    1:8 Average % 99    
    Range % 90-103    
  • 回收率：
    The recovery of mouse MDC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 99 89-103    
    EDTA plasma (n=4) 93 84-98    
                   
                   
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average Corrected    
    2000 2.888 2.812 2.850 2.683    
    1000 2.589 2.513 2.551 2.384    
    500 2.110 2.168 2.139 1.972    
    250 1.463 1.446 1.455 1.288    
    125 0.810 0.827 0.819 0.652    
    62.5 0.537 0.554 0.546 0.379    
    31.25 0.320 0.311 0.316 0.149    
    0 0.169 0.165 0.167 　    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 最新研究進展：
        可以簡單地將CCL22描述為一種趨化因子（chemokine），屬于家族中的CC型趨化因子。CCL22主要由單核細胞和樹突狀細胞產(chǎn)生，并在調(diào)節(jié)免疫細胞的遷移和定位中發(fā)揮作用。CCL22與其特定的受體CCR4結(jié)合，參與了過敏性疾病、炎癥和腫瘤的發(fā)生發(fā)展過程。最新研究表明，CCL22-CCR4信號通路的抑制可能有助于治療一些腫瘤類型。
     
  • 功能：
    Chemotactic for activated T-lymphocytes. May play an important role in the collaboration of dendritic cells and B-lymphocytes with T-cells in immune responses.
  • 基因功能參考文獻：
    1. the subset of peritoneal CD11b(+)CD169(+) macrophages increased and CCL22 expression level decreased significantly during the DSS-induced colitis PMID: 28466432
    2. Intratumoral administration of anti-CCL22 antibody inhibited B16F10 melanoma growth. PMID: 28668835
    3. this study shows that intravenous injection of apoptotic cells induces a subsequent increase in CCL22 expression and CCR4+ Treg cells, which contribute to the maintenance of immune homeostasis at least partially by splenic CD8alpha+ CD103+ dendritic cells PMID: 26868141
    4. CCL22-specific antibodies reveal that engagement of two distinct binding domains on CCL22 is required for CCR4-mediated function. PMID: 26683175
    5. CCL22 was localised mainly on the cell surface and or in the cytoplasm. Within sections of omental milky spot micrometastases, CCR4 was recognised on or in gastric cancer cells, constituent cells milky spots, blood cells and blood endothelial cells PMID: 25245466
    6. results show that the IL-4/CCL22/CCR4 axis is involved in the migration of Tregs to osteolytic lesion sites, and attenuates development of lesions by inhibiting inflammatory migration and the production of proinflammatory and osteoclastogenic mediators PMID: 25264308
    7. CCL22 has a role in Treg skin homing to suppress depigmentation PMID: 25634358
    8. CCL22 is a novel mediator of lung inflammation following hemorrhage and resuscitation PMID: 25136780
    9. The immunomodulatory properties of CCL22 could be harnessed for prevention of graft rejection and type 1 diabetes as well as other autoimmune disorders. PMID: 25740943
    10. Data indicate macrophage-derived chemokine CCL22 as an adjuvant could enhance the immune protective effect of NTHi-P6 protein vaccine to an extent. PMID: 25200152
    11. islet expression of CCL22 recruits Tregs and attenuates autoimmune destruction of beta cell PMID: 21737880
    12. Data show that the presence of the Ccr4 and Ccl22 transcripts were detected in brain slices. PMID: 21177120
    13. These results suggest that CCL22 functions to regulate development of experimental autoimmune encephalomyelitis through macrophage chemoattraction and effector function. PMID: 20940325
    14. role of CCL22 for the recruitment of eosinophils during allergic pleurisy PMID: 12629149
    15. functionally increased expression during Salmonella enterica serovar Typhimurium infection of dendritic cells PMID: 15731072
    16. Regulatory elements of the CCL22 gene required to mediate a strong and highly specific expression are included in a 4.1-kb promoter region, with the major elements active in dendritic cells and B cells being located in a small proximal 250-bp region. PMID: 15843561
    17. CCL22 was selectively upregulated in osteoclast-like cells derived from RAW264.7 cells and mouse bone marrow cells upon stimulation with RANKL (receptor activator of nuclear factor-kappaB ligand). PMID: 16821125
    18. IgE appears to be capable of stimulating basophils to produce MDC in the absence of a specific Ag, which may contribute to IgE-mediated and/or Th2-predominant allergic inflammation. PMID: 18832724
    19. When isolated, only natural killer (NK)-containing cellular fractions secrete CCL22, and the same fraction isolated from metastatic Lewis lung carcinoma (LLC)-bearing lungs secrete higher levels. PMID: 19234170
    20. Mice genetically deficient in CCR4 (the receptor for CCL22) show markedly reduced indoleamine dioxygenase (IDO) expression in MLN-DCs and support the involvement of the CCL22/CCR4 axis in IDO induction. PMID: 19843945

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Intercrine beta (chemokine CC) family
  • 組織特異性：
    Expressed by activated splenic B-lymphocytes and dendritic cells. Low expression in lung, thymocytes, lymph node, and unstimulated splenic cells.
  • 數(shù)據(jù)庫鏈接：