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Mouse Macrophage Inflammatory Protein 3α,MIP-3α ELISA kit

  • 中文名稱:
    小鼠巨噬細(xì)胞炎性蛋白3α(MIP-3α/CCL20)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-E04668m
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3200/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    小鼠巨噬細(xì)胞炎性蛋白3α(MIP-3α/CCL20)酶聯(lián)免疫試劑盒(CSB-E04668m)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的CCL20含量。CCL20是重要靶點(diǎn)。其背景上,它是一種趨化因子,在免疫調(diào)節(jié)等生理過(guò)程中發(fā)揮作用。研究機(jī)制方面,它能與特定受體結(jié)合,介導(dǎo)免疫細(xì)胞的遷移和活化,在炎癥反應(yīng)、腫瘤微環(huán)境等領(lǐng)域是研究熱點(diǎn),對(duì)相關(guān)疾病治療意義重大。試劑盒檢測(cè)范圍為39 pg/ml- 2500 pg/ml,靈敏度為9.75 pg/ml。可廣泛應(yīng)用于炎癥機(jī)制研究、免疫調(diào)節(jié)藥物開(kāi)發(fā)、感染性疾病模型評(píng)估等領(lǐng)域,為探索CCL20在Th17細(xì)胞募集、腸道屏障功能、病原體防御中的分子機(jī)制提供可靠工具,尤其適用于關(guān)節(jié)炎模型、結(jié)腸炎模型或呼吸道感染模型中趨化因子動(dòng)態(tài)變化的科研分析。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名:
    Ccl20 ELISA kit; Larc ELISA kit; Scya20C-C motif chemokine 20 ELISA kit; Beta-chemokine exodus-1 ELISA kit; CC chemokine LARC ELISA kit; CC chemokine ST38 ELISA kit; Liver and activation-regulated chemokine ELISA kit; Macrophage inflammatory protein 3 alpha ELISA kit; MIP-3-alpha ELISA kit; Small-inducible cytokine A20 ELISA kit
  • 縮寫(xiě):
  • Uniprot No.:
  • 種屬:
    Mus musculus (Mouse)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍:
    39 pg/ml - 2500 pg/ml
  • 靈敏度:
    9.75 pg/ml
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Immunology
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Sandwich
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評(píng)價(jià)

平均分:
5.0分 - 1 個(gè)評(píng)價(jià)

樣品類型:組織勻漿液 小鼠組織

樣品信息:小鼠

稀釋比:沒(méi)有稀釋

產(chǎn)品評(píng)價(jià): 我用CSB-E04668m試劑盒檢測(cè)小鼠的組織勻漿液,試劑盒發(fā)揮穩(wěn)定,建議在進(jìn)行正式實(shí)驗(yàn)前先摸索一下稀釋濃度。實(shí)驗(yàn)前比較擔(dān)心檢測(cè)不出或太高,但是最后結(jié)果不錯(cuò)。信賴華美,我用試劑盒的首選。

By 王老師

靶點(diǎn)詳情

  • 功能:
    Acts as a ligand for C-C chemokine receptor CCR6. Signals through binding and activation of CCR6 and induces a strong chemotactic response and mobilization of intracellular calcium ions. The ligand-receptor pair CCL20-CCR6 is responsible for the chemotaxis of dendritic cells (DC), effector/memory T-cells and B-cells and plays an important role at skin and mucosal surfaces under homeostatic and inflammatory conditions, as well as in pathology, including cancer and autoimmune diseases. CCL20 acts as a chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Involved in the recruitment of both the proinflammatory IL17 producing helper T-cells (Th17) and the regulatory T-cells (Treg) to sites of inflammation. Required for optimal migration of thymic natural regulatory T cells (nTregs) and DN1 early thymocyte progenitor cells. Positively regulates sperm motility and chemotaxis via its binding to CCR6 which triggers Ca2+ mobilization in the sperm which is important for its motility. May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells.
  • 基因功能參考文獻(xiàn):
    1. Obesity-induced IL-6 shifts macrophage polarization towards tumor-promoting macrophages that produce the CCL-20 in the colitis-associated colorectal cancer (CAC) microenvironment. CCL-20 promotes CAC progression by recruiting CCR6-expressing B-cells and gammadelta T cells via chemotaxis. PMID: 29695802
    2. Blocking or knockdown CCN1 expression ameliorated skin inflammation and reduced the expression of CCL20 in both imiquimod and IL-23-induced psoriasis-like mouse models. PMID: 28602508
    3. these data indicate that CCL20/CCR6 signaling may play an important role in regulating bone mass accrual, potentially by modulating osteoblast maturation, survival, and the recruitment of osteoblast-supporting cells. PMID: 26890063
    4. We crossed Ptf1a(Cre/+) ;Kras(G12D/+) mice with JNK1(-/-) mice to generate Ptf1a(Cre/+) ;Kras(G12D/+) ;JNK1(-/-) (Kras;JNK1(-/-) ) mice. Tumor weight was significantly lower in Kras;JNK1(-/-) mice than in Kras;JNK1(+/-) mice, whereas histopathological features were similar.we concluded that inhibition of activated JNK in pancreatic tumor stroma could be a potential therapeutic target to increase Ccl20 secretion PMID: 28837246
    5. mutually exclusive transcriptional regulation by AP-1 (cjun/cfos) and non-canonical NF-kappaB (RelB/p52) downstream of MEK-ERK and NIK-IKK-alpha-NF-kappaB2 (p100) phosphorylation, respectively was responsible for persistent Ccl20 expression in the colonic cells. PMID: 27590109
    6. Taken together, these data show that, in adipose tissues, IL-17A contributes to exacerbating insulin resistance-enhancing IL-6 production and promotes the infiltration of Th17 cells in cooperation with TNFalpha; these findings represent a novel hypothesis for the association between IL-17A-producing cells and type 2 diabetes. PMID: 27311858
    7. Data (including data from studies in knockout/mutant mice) suggest that expression of Ahr (aryl-hydrocarbon receptor) and its translocation into nucleus are necessary for Ahr ligand-mediated synergistic induction of Ccl20; here, TCDD is Ahr ligand. PMID: 26259605
    8. alpha-hemolytic streptococcus may exacerbate kidney damage in IgA nephropathy through CCL20 response to the effect of Th17 cells. PMID: 25265199
    9. Acquired mtDNA mutations may promote tumorigenic phenotypes through up-regulation of chemokine CCL20. PMID: 25177208
    10. Overexpression of CCL20 in human proximal tubular cells is inhibited by blockade of KCa3.1 under diabetic conditions through inhibition of the NF-kappaB pathway. PMID: 24733189
    11. Our data indicate that estradiol is important in regulating the effects of keratinocyte growth factor on mouse uterine epithelial cell secretion of CCL20 and CXCL1. PMID: 24807244
    12. CCL20 blockage lead to a diminished cerebral immune response in experimental pneumococcal meningitis. PMID: 24699535
    13. This study suggests that CCL20/IL-15 can induce a strong antitumor immune response in tumor tissues and it is a suitable candidate for cancer immunotherapy. PMID: 24657179
    14. Estrogen receptors alpha regulates CCL20/CXCL1 secretion in the female reproductive tract. PMID: 23025258
    15. Cardiac fibroblasts could recruit Th17 cells infiltration into myocardium by secreting CCL20 in acute viral myocarditis. PMID: 24296428
    16. Data indicate that a CCR6/CCL20 chemokine loop instructed rapid increase of B cells in the spleen in response to systemic administration of Nod1 agonists in a TNF-alpha-dependent manner. PMID: 24534531
    17. Neutralization of CCL20 before induction of sepsis increased mortality during sepsis accompanied with increasing epithelial apoptosis in the jejunum and augmenting serum TNF-alpha. PMID: 23601903
    18. CXCR3 promotes recruitment of Th17 cells from the blood into the liver in both human and murine liver injury. Their subsequent positioning near bile ducts is dependent on CCR6 and cholangiocyte-secreted CCL20. PMID: 22796894
    19. Findings suggest that TNF-alpha is essential in the induction of autoimmune hepatitis (AIH) through upregulation of hepatic CCL20 expression, which allows migration of dysregulated splenic T cells. PMID: 23178752
    20. Regulation of CCL20 expression in astrocytes is stimulated by the addition of interleukin (IL)-6, IL-6 soluble receptor, and IL-17. PMID: 22319003
    21. Tumor-associated macrophages recruit CCR6+ regulatory T cells and promote the development of colorectal cancer via enhancing CCL20 production in mice PMID: 21559338
    22. the healing response to corneal epithelial abrasion includes CCL20-dependent influx of CCR6(+) IL-17(+) IL-22(+) gammadelta T cells, and IL-22 contributes to the inflammatory response and promotes epithelial healing PMID: 21518851
    23. IL-9 is produced by several T helper (Th) cell subsets in the presence of IL-4 and induces CCL-20 production by astrocytes to induce the migration of Th17 cells into the central nervous system. PMID: 21346235
    24. Data show that the synergistic actions of pGM-CSF and pMIP3alpha presents a potentially feasible means of controlling immunogenic malignancies and provides a basis for the development of novel immunotherapeutic treatments. PMID: 20804501
    25. CCL20 is important in mediating leukocyte recruitment early upon infection with respiratory syncytial virus. PMID: 20101616
    26. Interfering with CCL20 significantly downregulates the expression of CD4(+)CD25(+) T cell development in mouse thymus. PMID: 18315924
    27. through secretion of CCL20, astrocytes could play an important role in orchestrating the recruitment of specific leukocyte subsets to the inflamed CNS and in regulating CNS-targeted immune responses. PMID: 12528183
    28. In experimental autoimmune encephalitis MIP-3 alpha is required for sensitization of T cells to antigen and for release of T cells from lymph nodes during the course of an immune response. PMID: 12794163
    29. CCL20 plays a vital role in B-cell adhesion to the inflamed endothelium PMID: 12816871
    30. our findings indicate that blockade of MIP-3alpha bioactivity can significantly reduce TNBS-mediated colonic injury and T cell recruitment, suggesting a role for this chemokine in the pathophysiology of intestinal inflammation. PMID: 17272517
    31. CXCL12-CXCR4 and CCL20-CCR6 systems are involved in T lymphocyte-endothelial interaction in microvessels of the small and large intestines. PMID: 17885999
    32. The joint cytokine milieu formed by T cells and synovial cells controls the production of CCL20 and, consequently, the recruitment of chemokine receptor 6 arthritogenic Th cells to the inflamed joints. PMID: 18025126
    33. Our studies suggest that TLR4 expressed by tumor cells may be involved in the induction of chemokines like CCL20. PMID: 18083111
    34. Th17 cytokines stimulate CCL20 production in vitro and in vivo, and thus provide a potential explanation of how CCR6-positive Th17 cells maintain their continual presence in psoriasis through a positive chemotactic feedback loop. PMID: 19295614
    35. Investigated the regulation of ccl20 expression and found different NF-kappaB pathways modulate CCL20 transcription by operating on the same NF-kappaB binding site in the same cell type. PMID: 19303953
    36. the CCR6-CCL20 axis in the choroid plexus controls immune surveillance of the CNS. PMID: 19305396

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  • 亞細(xì)胞定位:
    Secreted.
  • 蛋白家族:
    Intercrine beta (chemokine CC) family
  • 組織特異性:
    Thymic medulla (at protein level). Prominently expressed in the small intestine, colon and appendix. Also found in thymus, spleen, lymph node and lung. The long form might be dominant in intestinal, and the short form in lymphoid tissues. Expressed by IL1
  • 數(shù)據(jù)庫(kù)鏈接: