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  4. Mouse Macrophage Migration Inhibitory Factor,MIF ELISA Kit

Mouse Macrophage Migration Inhibitory Factor,MIF ELISA Kit

  • 中文名稱：
    小鼠巨噬細(xì)胞移動(dòng)抑制因子(MIF)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E07292m
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3200/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠巨噬細(xì)胞移動(dòng)抑制因子(MIF)酶聯(lián)免疫試劑盒（CSB-E07292m）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的MIF含量。MIF即巨噬細(xì)胞移動(dòng)抑制因子，是一種多功能細(xì)胞因子。它在炎癥、免疫反應(yīng)及腫瘤發(fā)生發(fā)展等過程中扮演重要角色。研究機(jī)制上，MIF可與多種受體結(jié)合，激活下游信號(hào)通路，調(diào)控細(xì)胞的增殖、凋亡和炎癥因子釋放等，是極具潛力的治療靶點(diǎn)。試劑盒檢測(cè)范圍為125 pg/ml- 8000 pg/ml，靈敏度為31.25 pg/ml。適用于基礎(chǔ)醫(yī)學(xué)研究中炎癥機(jī)制探索、免疫調(diào)控實(shí)驗(yàn)驗(yàn)證，以及心血管疾病、代謝綜合征等動(dòng)物模型的生物標(biāo)志物分析，可廣泛用于藥物開發(fā)過程中抗炎化合物或免疫調(diào)節(jié)劑的體外效果評(píng)估。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    MifMacrophage migration inhibitory factor ELISA Kit; MIF ELISA Kit; EC 5.3.2.1 ELISA Kit; Delayed early response protein 6 ELISA Kit; DER6 ELISA Kit; Glycosylation-inhibiting factor ELISA Kit; GIF ELISA Kit; L-dopachrome isomerase ELISA Kit; L-dopachrome tautomerase ELISA Kit; EC 5.3.3.12 ELISA Kit; Phenylpyruvate tautomerase ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    125 pg/ml - 8000 pg/ml
  • 靈敏度：
    31.25 pg/ml
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse MIF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    100

    Range %

    95-105

    1:2

    Average %

    97

    Range %

    91-105

    1:4

    Average %

    97

    Range %

    92-107

    1:8

    Average %

    92

    Range %

    86-98

  • 回收率：

    The recovery of mouse MIF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    102

    99-110

    EDTA plasma (n=4)

    97

    90-106

  • 標(biāo)準(zhǔn)曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    8000

    2.326

    2.434

    2.380

    2.212

    4000

    1.818

    1.767

    1.793

    1.625

    2000

    1.243

    1.228

    1.236

    1.068

    1000

    0.759

    0.729

    0.744

    0.576

    500

    0.417

    0.421

    0.419

    0.251

    250

    0.327

    0.333

    0.330

    0.162

    125

    0.263

    0.274

    0.269

    0.101

    0

    0.171

    0.165

    0.168

    　

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 最新研究進(jìn)展：
    MIF是一種促炎癥因子，全名為遷移抑制因子。它參與免疫反應(yīng)、炎癥反應(yīng)、細(xì)胞增殖等多種生理過程，在多種疾病的發(fā)生和發(fā)展中發(fā)揮重要作用。最近的研究表明，MIF與腫瘤、炎癥性疾病、自身免疫性疾病等多種疾病的發(fā)生和發(fā)展密切相關(guān)。
  • 功能：
    Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.
  • 基因功能參考文獻(xiàn)：
    1. MIF mediates LPS-induced cardiac dysfunction in murine cardiomyocytes, which was attenuated by MIF knockout. PMID: 29350381
    2. MIF attenuates oxygen-glucose deprivation-induced cochlear cells injury. MIF enhances Nrf2 and inhibit oxidative stress in cochlear cells. Enhanced Akt-Nrf2-HO-1 pathway may mediate cochlear protection by MIF. PMID: 29908183
    3. Data indicate function of macrophage migration inhibitory factor (MIF) as a regulator of the NLR family pyrin domain containing 3 (NLRP3) inflammasome complex in macrophages. PMID: 29884801
    4. that macrophage migration inhibitory factor directly engages in dengue NS1-induced glycocalyx degradation and that targeting MIF may represent a possible therapeutic approach for preventing dengue-induced vascular leakage PMID: 29702687
    5. our data suggests a model in which MIF expression in the primary tumor dampens the anti-tumor immune response, promoting tumor growth PMID: 29864117
    6. MIF knockdown significantly accentuates hearing loss in young mice. PMID: 28990052
    7. Mif mediates PAR4-induced bladder pain through urothelial HMGB1. PMID: 29263120
    8. These results show although high systemic levels of MIF contribute to the development of type 2 diabetes mellitus pathology. PMID: 28780379
    9. High MIF expression is associated with progressive multiple sclerosis. PMID: 28923927
    10. The lack of MIF leads to disturbances of systemic and hippocampal insulin sensitivity, which are possibly responsible for memory deficits and anxiety, most likely through decreased PSA-NCAM-mediated neuroplasticity rather than through neurotrophic factors. PMID: 28919555
    11. These data indicate the functional role of the MIF-COX-p53 axis in inflammation and cancer at the genomic and proteomic levels in COX-2-ablated cells. PMID: 29247872
    12. Our results showed that MIF regulates MCP-1 expression in hepatocytes of injured liver via CD74, CD44, and p38 MAPK in an autocrine manner. PMID: 27273604
    13. MIF is involved in the pathogenesis of AF, probably by down-regulating the protein and gene expression of Cx43 via ERK1/2 kinase activation PMID: 28429502
    14. Endogenous MIF reduces the accumulation and toxicity of misfolded SOD1 in a mouse model of amyotrophic lateral sclerosis. PMID: 27551074
    15. Gene expression of MIF was 30-fold higher in the heart, compared to skeletal muscle and protein expression of MIF was 3-fold higher in the heart compared to skeletal muscle. PMID: 27364992
    16. renal tubular MIF is an endogenous renoprotective factor in progressive kidney diseases PMID: 28801314
    17. locally produced MIF in the inflammatory bone lytic site is engaged in the chemoattraction of circulating CXCR4+ osteoclast precursor cells. PMID: 27082509
    18. MIF expression was induced in chondrocytes of tissue-engineered cartilage, and could exert a profound effect on chondrocytes by promoting cartilage maturation. MIF could also regulate the phenotype of surrounding macrophages, impairing the maturation of transplanted tissues. PMID: 28574571
    19. pretreatment of P. aeruginosa with rMIF is associated with reduced bacterial killing by tobramycin. PMID: 28768722
    20. loss of autophagy, by pharmacological inhibition or siRNA silencing of Atg5, enhances MIF secretion by monocytes and macrophages. PMID: 27163877
    21. CHD7 is an important factor in the proliferation and stemness maintenance of neural stem/progenitor cells. PMID: 27955690
    22. MIF-deficient mice have reduced Nippostrongylus brasiliensis burden and mounted an enhanced type 2 immune response, including increased Gata3 expression and interleukin-13 production in the mesenteric lymph nodes PMID: 27049059
    23. Sertoli cells to produce MIF under normal conditions. MIFR is expressed in GFRalpha1 and Sertoli cells. MIF induced spermatogonial cell migration PMID: 27925200
    24. MIF-transgenic cells exhibited substantially decreased levels of p53 after hyperthermia treatment compared with WT and MIF-knockout cells PMID: 27528627
    25. This study showed that loss of keratinocyte-derived MIF leads to a loss of control of epithelial skin tumor formation in chemical skin carcinogenesis, which highlights an unexpected tumor-suppressive activity of MIF in murine skin. PMID: 27825106
    26. This study was undertaken to investigate the potential role of Macrophage migration inhibitory factor in osteoarthritis in human joint tissues and in vivo in mice with age-related and surgically induced osteoarthritis PMID: 27564840
    27. MIF (macrophage migrating inhibitory factor), a potential pathogenic molecule in African trypanosomosis, was found herein to promote erythrophagocytosis, to block extramedullary erythropoiesis and RBC maturation, and to trigger hemodilution. PMID: 27632207
    28. findings suggest that macrophage migration inhibitory factor regulates extramedullary erythropoiesis by inhibiting an overexpansion of splenic immature erythroid cells during chronic stress and indicate a novel role for this cytokine under chronic stress conditions PMID: 27129368
    29. Findings suggest that Mif plays a role in the molecular mechanisms of macrophage and dendritic cell activation and drives T cell responses involved in the pathology of type 1 diabetes mellitus. PMID: 27699180
    30. MIF has a potential role in pathological angiogenesis of proliferative retinopathy. PMID: 28070752
    31. genetic Mif deletion reduces the incidence and severity of oral carcinogenesis, by inhibiting the expression of chronic pro-inflammatory immune mediators. Thus, targeting MIF is a promising strategy for the prevention or therapy of oral cancer. PMID: 27164411
    32. MIF inhibits the myoblast differentiation by affecting the cell cycle progression, but does not affect proliferation. PMID: 26927414
    33. this paper shows that the detrimental effect of MIF knockout was associated with accentuated loss in cardiac autophagy with aging PMID: 26940544
    34. our results suggest that MIF promotes mCSC survival, proliferation and endothelial differentiation through the activation of the PI3K/Akt/mTOR and AMPK signaling pathways. PMID: 27035848
    35. Posttranslational modification of MIF by S-nitrosation results in intracellular accumulation and protection from myocardial ischemia reperfusion injury. PMID: 26310191
    36. Data show that the siRNA-induced macrophage migration inhibitory factor (MIF) reduction in murine mammary cancer line 4T1 and human breast cancer line MDA-MB-231 resulted in significant reduction of cell proliferation and increase of apoptosis. PMID: 26403072
    37. High expression levels of macrophage migration inhibitory factor sustain the innate immune responses of neonates. PMID: 26858459
    38. The deletion of the MIF gene led to reduced behavioural despair in mice of both sexes and IFN-gamma mRNA levels were reduced in the hippocampus of the MIF KO mice. PMID: 26338025
    39. In D-galactosamine-sensitized mice CP+Cu(II) increased the LPS-induced lethality from 54 to 100%, while administration of antibodies against MIF prevented the lethal effect. The enhancement by CP+Cu(II) of the pro-inflammatory signal of MIF is discussed. PMID: 26091949
    40. data suggest that the MIF-Notch axis may play an important role in the pathogenesis of experimental autoimmune uveitis PMID: 26400205
    41. The functional role of MIF in cell recruitment was investigated by a chemotaxis assay and by flow cytometry of labeled macrophages that were injected into Mif-/-and wildtype mice PMID: 26348853
    42. these results implicate MIF in the pathogenesis of esophageal inflammation and suggest that targeting MIF might represent a novel therapy for EoE. PMID: 25712805
    43. Data suggest that macrophage migration inhibitory factor (MIF) inhibition could be a promising approach to the treatment of diabetes mellitus (DM)-associated atherosclerosis (AS). PMID: 25661015
    44. Bladder PAR activation elicits urothelial MIF release and urothelial MIF receptor signaling at least partly through CXCR4 to result in abdominal hypersensitivity without overt bladder inflammation PMID: 26020638
    45. Transcription factor MEF2 and Zac1 mediate MIF-induced GLUT4 expression through CD74-dependent AMPK activation in cardiomyocytes PMID: 26455966
    46. Blockade of CXCR7 suppressed MIF-mediated ERK- and zeta-chain-associated protein kinase (ZAP)-70 activation PMID: 26139098
    47. Macrophage migration inhibitory factor is detrimental for survival and is associated with lung pathology, inflammatory cellular infiltration, and bacterial replication in a mouse model of pneumococcal pneumonia. PMID: 25943202
    48. Macrophage migration inhibitory factor may play an important role in recovery from acoustic trauma PMID: 25853607
    49. data indicate that MIF and CD74 facilitate RANKL-induced osteoclastogenesis, and suggest that MIF contributes directly to bone erosion, as well as inflammation, in rheumatoid arthritis PMID: 25647268
    50. MIF was found to be a major platelet-derived chemotactic recruitment factor with clot-modulating properties and therefore might be relevant in inflammatory diseases such as atherosclerosis PMID: 25561410

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  • 亞細(xì)胞定位：
    Secreted. Cytoplasm.
  • 蛋白家族：
    MIF family
  • 數(shù)據(jù)庫鏈接：

    KEGG: mmu:17319

    STRING: 10090.ENSMUSP00000041149

    UniGene: Mm.2326