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  4. Mouse Membrane primary amine oxidase(AOC3) ELISA kit

Mouse Membrane primary amine oxidase(AOC3) ELISA kit

  • 中文名稱：
    小鼠伯胺氧化酶膜(AOC3)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL001855MO
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠伯胺氧化酶膜(AOC3)酶聯(lián)免疫試劑盒（CSB-EL001855MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的AOC3含量。AOC3作為靶點，是含氨氧化酶家族成員，主要分布在白細(xì)胞等細(xì)胞表面。其作用機(jī)制與細(xì)胞黏附、遷移等過程相關(guān)。目前研究聚焦于其在免疫調(diào)節(jié)、炎癥反應(yīng)等病理生理過程中的角色，有望為相關(guān)疾病的治療提供新方向。試劑盒檢測范圍為1.25 ng/mL-80 ng/mL，本試劑盒適用于科研場景下的小鼠模型研究，如通過檢測血清或血漿樣本評估循環(huán)系統(tǒng)中AOC3的動態(tài)變化，或通過組織勻漿分析特定器官（如肝臟、脂肪組織）中該蛋白的表達(dá)特征，為炎癥機(jī)制、藥物干預(yù)效果或免疫調(diào)節(jié)研究提供定量分析工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Aoc3; Vap1; Membrane primary amine oxidase; Copper amine oxidase; Semicarbazide-sensitive amine oxidase; SSAO; Vascular adhesion protein 1; VAP-1
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    1.25 ng/mL-80 ng/mL
  • 靈敏度：
    0.31 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse AOC3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %83
    Range %80-86
    1:2Average %90
    Range %85-96
    1:4Average %96
    Range %93-99
    1:8Average %104
    Range %100-109
  • 回收率：
    The recovery of mouse AOC3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9590-100
    EDTA plasma (n=4)9794-100
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    802.507 2.569 2.538 2.351
    401.662 1.600 1.631 1.444
    200.999 0.968 0.984 0.797
    100.587 0.572 0.580 0.393
    50.444 0.458 0.451 0.264
    2.50.354 0.362 0.358 0.171
    1.250.279 0.298 0.289 0.102
    00.188 0.186 0.187
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Cell adhesion protein that participates in lymphocyte recirculation by mediating the binding of lymphocytes to peripheral lymph node vascular endothelial cells in an L-selectin-independent fashion. Has a monoamine oxidase activity. May play a role in adipogenesis.
  • 基因功能參考文獻(xiàn)：
    1. Data indicate strong-to-moderate expression of vascular adhesion protein-1 (VAP-1) in the synovium of Borrelia burgdorferi-infected mice, while only weak expression of VAP-1 was detected in uninfected mice. PMID: 29166944
    2. VAP-1 was expressed on endothelial cells lining inflamed atherosclerotic lesions; normal vessel walls in aortas of C57BL/6N control mice were VAP-1-negative. PMID: 27731409
    3. VAP-1 plays a critical role in the pathophysiology of renal ischemia/reperfusion injury by enhancement of neutrophil infiltration generating a local hydrogen peroxide gradient. PMID: 28318627
    4. This study showed that the oxidase activity of AOC3 contributes to the development of lung fibrosis mainly by regulating the accumulation of pathogenic leukocyte subtypes, which drive the fibrotic response. PMID: 28251930
    5. data indicate that SSAO inactivation in vivo stabilizes the established plaques mainly via inducing the switch of SMCs from a contractile to a synthetic phenotype PMID: 27043821
    6. Suggest inhibiting VAP-1/SSAO enzymatic function, by PXS-4728A, as a novel therapeutic approach in lung diseases that are characterized by neutrophilic pattern of inflammation. PMID: 25889951
    7. these results link the amine oxidase activity of VAP-1 with hepatic inflammation and fibrosis and suggest that targeting VAP-1 has therapeutic potential for NAFLD and other chronic fibrotic liver diseases. PMID: 25562318
    8. these results suggest a largely redundant function for AOC3/VAP-1 in allergic inflammatory responses of the airways. PMID: 25116373
    9. The results of the study establish VCAM-1 and VAP-1 as mediators of myeloid cell recruitment in metastasis and identify VAP-1 as a potential target for therapeutic intervention to combat early metastasis. PMID: 23407548
    10. The antihyperglycemic effect of benzylamine is abolished by AOC3 gene knockout in mice. PMID: 22547093
    11. Long-term activation of semicarbazide-sensitive amine oxidase by benzylamine lowers circulating levels of uric acid in diabetic conditions. PMID: 22480418
    12. AOC3 expression is increased in perigonadal and subcutaneous adipose tissue of diabetic db-/- obese mice. PMID: 21298297
    13. This review examines the biological functions of VAP-1, especially the role that this molecule might play in the establishment and progression of chronic liver disease. PMID: 21512782
    14. The demonstrated AOC3 turnover of primary amines that are non-native to human tissue suggests possible roles for the adipocyte enzyme in subcutaneous bacterial infiltration and obesity. PMID: 22238597
    15. VAP-1-mediated M2 macrophage infiltration underlies IL-1beta- but not VEGF-A-induced lymph- and angiogenesis PMID: 21435467
    16. VAP-1 inhibition provided antiinflammation effect by reducing adhesion molecule expression and immune cell infiltration after intracerebral hemorrhage. PMID: 20877383
    17. histamine moderately activated lipolysis in adipocytes in AOC3 knockout mice PMID: 20012150
    18. Enzyme is in involved in deamination in atherogenesis in KKAy diabetic mice fed with high cholesterol diet. PMID: 12242458
    19. the increased enzyme activity observed in diabetes is not a result of increased SSAO gene transcription. It is controlled by posttranslational modifications of the protein, and the catalytic activity controls the gene expression PMID: 12606817
    20. vascular adhesion protein-1 expression may contribute to the functional heterogeneity of endothelial cells within the lung to create distinct sites for the recruitment of inflammatory cells PMID: 12700900
    21. Release of this enzyme is regulated by TNF-alpha and insulin and, in adipose cells could contribute to the atherogenesis and vascular dysfunction associated with diabetes and obesity. PMID: 14968297
    22. The vascular endothelium is the major source of circulating VAP-1 protein and semicarbazide-sensitive amine oxidase activity. PMID: 15178639
    23. VAP-1 mediates leukocyte trafficking to sites of inflammation and thus is a potential target for anti-inflammatory therapies PMID: 15743791
    24. T helper type 2 cells use Vap-1 to roll and adhere in the inflamed liver microcirculation PMID: 16111634
    25. These data demonstrate the potential clinical benefit of small molecule anti-SSAO therapy in this mouse multiple sclerosis model. PMID: 17393060
    26. The effects of exogenous or biogenic amines on glucose transport are not receptor-mediated but are oxidation-dependent. The major SSAO form expressed in mouse adipocytes is encoded by the AOC3 gene. PMID: 17406965
    27. VAP-1-deficient mice have mild deviations in the mucosal immune system PMID: 17947691
    28. The atheroprotective effect of LXR agonist T0901317 is related to the inhibition of SSAO gene expression and its activity. PMID: 18330481
    29. LOX binds to mature TGF-beta1 and enzymatically regulates its signaling in bone via amine oxidase activity, and thus may play an important role in bone maintenance and remodeling PMID: 18835815
    30. VAP-1 regulates the inflammatory response in ischemia-reperfusion injury and suggest that blockade of VAP-1 may have therapeutic value. PMID: 18991279

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  • 亞細(xì)胞定位：
    Membrane; Single-pass type II membrane protein.
  • 蛋白家族：
    Copper/topaquinone oxidase family
  • 數(shù)據(jù)庫鏈接：