Mouse N-terminal procollagen Ⅲ propeptide(PⅢNP) ELISA Kit
-
中文名稱:小鼠III型前膠原氨基末端肽(PⅢNP)酶聯(lián)免疫試劑盒
-
貨號:CSB-E13334m
-
規(guī)格:96T/48T
-
價(jià)格:¥3600/¥2500
-
其他:
產(chǎn)品詳情
-
產(chǎn)品描述:小鼠III型前膠原氨基末端肽(PⅢNP)酶聯(lián)免疫試劑盒(CSB-E13334m)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的PⅢNP含量。PⅢNP(Ⅲ型前膠原氨基端肽)是一種纖維化指標(biāo),其水平與心室重塑程度相關(guān),對評估慢性心力衰竭(CHF)的嚴(yán)重程度有重要指導(dǎo)意義。研究發(fā)現(xiàn),PⅢNP水平與Gal-3(半乳糖凝集素3)水平呈顯著正相關(guān),提示PⅢNP可能通過促進(jìn)組織纖維化參與CHF的發(fā)生發(fā)展。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL,適用于體外研究纖維化疾病模型中的膠原代謝動(dòng)態(tài)、評估抗纖維化藥物干預(yù)效果,以及干細(xì)胞治療或組織工程領(lǐng)域中對膠原合成活性的監(jiān)測,為探索纖維化發(fā)病機(jī)制及治療策略提供可靠工具本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
-
別名:N-terminal procollagen 3 propeptide(P3NP),_x000D_ N-terminal procollagen III propeptide(PIIINP)
-
縮寫:PⅢNP
-
種屬:Mus musculus (Mouse)
-
樣本類型:serum, plasma, cell culture supernates, tissue homogenates
-
檢測范圍:0.312 ng/mL-20 ng/mL
-
靈敏度:0.078 ng/mL
-
反應(yīng)時(shí)間:1-5h
-
樣本體積:50-100ul
-
檢測波長:450 nm
-
研究領(lǐng)域:Others
-
測定原理:quantitative
-
測定方法:Sandwich
-
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse PIIINP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 89 Range % 86-92 1:2 Average % 94 Range % 91-97 1:4 Average % 100 Range % 95-104 1:8 Average % 104 Range % 100-108 -
回收率:
The recovery of mouse PIIINP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 94 90-97 EDTA plasma (n=4) 95 92-98 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average Corrected 20 2.569 2.499 2.534 2.381 10 1.798 1.768 1.783 1.630 5 1.298 1.246 1.272 1.119 2.5 0.854 0.898 0.876 0.723 1.25 0.474 0.498 0.486 0.333 0.625 0.371 0.398 0.385 0.232 0.312 0.222 0.233 0.228 0.075 0 0.152 0.154 0.153 -
數(shù)據(jù)處理:
-
貨期:3-5 working days
引用文獻(xiàn)
- A cyclic peptide-grafted Fc with hepatocyte growth factor functionality ameliorates hepatic fibrosis in a non-alcoholic steatohepatitis mouse model NM Rojas,/,2024
- Association of novel markers of liver disease with neonatal liver disease in premature baboons, Papio sp. LM Keller,PLoS One,2020
- Cordyceps cicadae NTTU 868 mycelium prevents CCl4-induced hepatic fibrosis in BALB/c mice via inhibiting the expression Bo-JunKe.et al,Journal of Functional Foods,2018
相關(guān)產(chǎn)品
Most popular with customers
-
Human Transforming Growth factor β1,TGF-β1 ELISA kit
Detect Range: 23.5 pg/ml-1500 pg/ml
Sensitivity: 5.8 pg/ml
-
-
-
Mouse Tumor necrosis factor α,TNF-α ELISA Kit
Detect Range: 7.8 pg/ml-500 pg/ml
Sensitivity: 1.95 pg/ml
-
-
-
-