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  4. Mouse NAD(P)H dehydrogenase [quinone] 1 (NQO1) ELISA kit

Mouse NAD(P)H dehydrogenase [quinone] 1 (NQO1) ELISA kit

  • 中文名稱：
    小鼠NAD(P)H脫氫酶[醌]1(NQO1)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL016039MO
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠NAD(P)H脫氫酶[醌]1(NQO1)酶聯(lián)免疫試劑盒（CSB-EL016039MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的NQO1含量。NQO1即醌氧化還原酶1，是一種重要的抗氧化酶。它廣泛存在于人體組織中，能參與醌類化合物代謝，在解毒、抗氧化等方面發(fā)揮關(guān)鍵作用。研究發(fā)現(xiàn)其與腫瘤發(fā)生發(fā)展密切相關(guān)，常被作為腫瘤治療潛在靶點，通過調(diào)控它來干預(yù)腫瘤進程。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，該產(chǎn)品為科研人員研究氧化應(yīng)激相關(guān)分子機制、建立疾病模型或評估藥物/化合物對NQO1調(diào)控作用提供可靠工具，特別適用于探索NQO1在代謝性疾病、癌癥或神經(jīng)保護中的功能，以及篩選影響該酶活性的天然產(chǎn)物或合成化合物。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Nqo1 ELISA Kit; Dia4 ELISA Kit; Nmo1 ELISA Kit; Nmor1 ELISA Kit; NAD(P)H dehydrogenase [quinone] 1 ELISA Kit; EC 1.6.5.2 ELISA Kit; Azoreductase ELISA Kit; DT-diaphorase ELISA Kit; DTD ELISA Kit; Menadione reductase ELISA Kit; NAD(P)H:quinone oxidoreductase 1 ELISA Kit; Phylloquinone reductase ELISA Kit; Quinone reductase 1 ELISA Kit; QR1 ELISA Kit
  • 縮寫：
    NQO1
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    31.25 pg/mL-2000 pg/mL
  • 靈敏度：
    7.81 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse NQO1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %97
    Range %92-104
    1:2Average %94
    Range %88-97
    1:4Average %84
    Range %80-88
    1:8Average %89
    Range %83-97
  • 回收率：
    The recovery of mouse NQO1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9386-98
    EDTA plasma (n=4)9994-106
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    20002.705 2.600 2.653 2.498
    10002.304 2.371 2.338 2.183
    5001.659 1.758 1.709 1.554
    2500.794 0.795 0.795 0.640
    1250.495 0.522 0.509 0.354
    62.50.352 0.357 0.355 0.200
    31.250.207 0.224 0.216 0.061
    00.150 0.160 0.155 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.
  • 基因功能參考文獻：
    1. Although the exact role of NQO1 in prostatic hyperplasia remains unclear, the hyperplasia exacerbation due to NQO1 deletion might be independent of type 2 5alpha-reductase and might be related to enhanced androgen receptor affinity due to enhanced HSP90-alpha expression. PMID: 29110315
    2. Novel RNA-binding activity of NQO1 promotes SERPINA1 mRNA translation. PMID: 27515817
    3. NQO1 and autophagy participate in a protective role in cisplatin injury. PMID: 26935540
    4. Miroestrol restored hepatic NQO1 expression in beta-naphthoflavone-treated mice. PMID: 26893163
    5. previous and present results collectively show that NQO1 is involved in the formation of tight junctions in the small intestine, and that defects in NQO1 enhance C. difficile toxin A-induced acute inflammatory responses, presumably via the loss of epithelial cell tight junctions PMID: 27116994
    6. NQO1 plays a critical role in protection against energy depletion in acetaminophen-induced liver injury, and is associated with improvement of mitochondrial dysfunction PMID: 25224400
    7. The present results demonstrate that exacerbated cisplatin-induced nephrotoxicity under the NQO1-knockout condition was accompanied by the reduced expression of MRN complex proteins, ATM, PARP1, and Sirt1. PMID: 26723870
    8. We defined the basal and butylated hydroxyanisole induced expression patterns of Nqo1, AKR1B8, and Ho-1 in the liver and small intestine of C57BL/6 mice. PMID: 26291391
    9. The colons of NQO1-KO mice also showed high levels of reactive oxygen species (ROS) and histone deacetylase (HDAC) activity, which are known to affect transcriptional regulation. PMID: 24393524
    10. induction by humulusol PMID: 24397993
    11. the induction of cellular NAD(+) levels using beta-lapachone (beta-Lap), whose intracellular target is NQO1, prevents the toxic effects of cisplatin through the regulation of PARP-1 and SIRT1 activity. PMID: 24922076
    12. Nqo1 expression is protective against renal ischemia/reperfusion injury in mice. PMID: 24189322
    13. results indicate that AAI can increase its own metabolic activation by inducing NQO1, thereby enhancing its own genotoxic potential. PMID: 24769487
    14. PCB-77 induced reductions in insulin signaling in adipose tissue were also abolished by Resveratrol, which increased NQO1 expression. PMID: 24231106
    15. NQO1 does not play a major role in the production of vitamin K hydroquinone and supports the existence of multiple vitamin K reduction pathways. PMID: 24015818
    16. NQO1 protects cells against renal failure induced by cisplatin; this effect is mediated by decreased NOX activity via cellular NADPH/NADP modulation. PMID: 23831944
    17. findings link NQO1, a cellular redox sensor, to the metabolite-sensing network that tunes PGC-1alpha expression and activity in regulating energy metabolism PMID: 23648480
    18. In the present study we show that [a] NQO1 protein locates to cytosol, ER and mitochondria. PMID: 23692925
    19. the results demonstrate for the first time that NQO1 is an endogenous factor in protection against painful bladder syndrome PMID: 22985937
    20. NADPH:quinone oxidoreductase 1 regulates host susceptibility to ozone via isoprostane generation PMID: 23275341
    21. hypertrophy is negatively regulated by NQO1 activity mouse 3T3-L1 adipocytes. PMID: 22683604
    22. NAD(P)H quinone oxidoreductase 1 regulates neutrophil elastase-induced mucous cell metaplasia. PMID: 22659878
    23. expression in cochlea did not decrease with age PMID: 21631178
    24. PGI synthase overexpression protects against bleomycin-induced mortality and is associated with increased Nqo 1 expression. PMID: 21764988
    25. NQO1 plays an important role in renal AAI nitroreduction and may thus contribute to AAI-induced nephrotoxicity. PMID: 21613233
    26. catalase overexpression upregulates BaP-induced NQO1 expression by enhancing the Sp1-AhR-Nrf2 signaling cascade. PMID: 21569840
    27. NQO1 activation has a hypotensive effect mediated by eNOS activation via cellular NAD(+)/NADH ratio modulation in an animal model. PMID: 21502369
    28. Hyperhomocysteinemia leads to a decrease of hepatic Nqo1 gene expression and activity with a reversal effect of wine polyphenol extract supplementation. PMID: 21550378
    29. Plasma membrane electron transport in pancreatic beta-cells is mediated in part by NQO1. PMID: 21505151
    30. findings assign NQO1 an original role in the regulation of mRNA translation via the control of eukaryotic translation initiation factor 4GI stability by the proteasome. PMID: 20028737
    31. NQO1 regulates p53 proteasomal degradation independently of MDM2 and ubiquitin. PMID: 12232053
    32. Induction of NQO1 activity exaggerates neuronal damage in a mouse focal cerebral ischaemia model PMID: 12603827
    33. NQO1 has an important role in stabilizing hot-spot p53 mutant proteins in cancer PMID: 14634213
    34. A role is suggested for NQO1 in myelination in the central nervous system or in the insulating/wrapping function of glial cells. PMID: 14991562
    35. These results indicate that one division of both the accessory and the main olfactory projection maps are composed of sensory neurons that are specialized to reduce environmental and/or endogenously produced quinones via an NQO1-dependent mechanism. PMID: 15128404
    36. NQO1 has a role in regulating ubiquitin-independent degradation of ornithine decarboxylase by the 20S proteasome PMID: 15749015
    37. This is the first demonstration that heavy metals (mercury, lead and copper) regulate the expression of Noqo1 gene through a transcriptonal mechanism. PMID: 16243960
    38. NQO1 plays a pivotal role in signaling activated by TNF and other inflammatory stimuli and that its suppression is a potential therapeutic strategy for tumor cells. PMID: 16682409
    39. NQO1 and NQO2 are endogenous factors in the regulation of immune response and autoimmunity PMID: 16905546
    40. NOS2 induction and NO production may be involved in TNF-alpha- and LPS-mediated downregulation of Cyp1a1 without affecting the downregulation of Nqo1. PMID: 17588258
    41. A major in vivo role of NQO1 in metabolic activation of mitomycin C with implications in mitomycin C chemotherapy. PMID: 17804703
    42. Bach1 repression of ferritin and thioredoxin reductase1 is heme-sensitive in cells and in vitro and coordinates expression with heme oxygenase1, beta-globin, and NADP(H) quinone (oxido) reductase1. PMID: 17901053
    43. Drug metabolizing enzyme induction of Nqo1 is reported in experimental fatty liver. PMID: 18488193
    44. The lack of induction of apoptosis and differentiation contributed to radiation-induced myeloproliferative disease in NQO1(-/-) mice. PMID: 18829548
    45. NQO1 modulates cellular redox status and influences the biologic and physiologic effects of ozone. PMID: 19059883
    46. It is concluded that cisplatin markedly increases the sensitivity of cancer to beta-lap in vitro and in vivo by upregulating NQO1. PMID: 19738461

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  • 亞細胞定位：
    Cytoplasm.
  • 蛋白家族：
    NAD(P)H dehydrogenase (quinone) family
  • 數(shù)據(jù)庫鏈接：

    KEGG: mmu:18104

    STRING: 10090.ENSMUSP00000003947

    UniGene: Mm.252