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  4. Mouse Noradrenaline,NA ELISA Kit

Mouse Noradrenaline,NA ELISA Kit

  • 中文名稱：
    小鼠去甲腎上腺素(NA)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E07870m
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠去甲腎上腺素(NA)酶聯(lián)免疫試劑盒（CSB-E07870m）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿樣本中的NA含量。小鼠去甲腎上腺素（NA）作為一種重要的神經(jīng)遞質(zhì)，在中樞神經(jīng)系統(tǒng)和交感神經(jīng)系統(tǒng)中參與調(diào)節(jié)生理過程，如感覺信號(hào)、注意力、清醒與睡眠、學(xué)習(xí)與記憶等。其釋放或信號(hào)傳遞的受損與精神疾病和神經(jīng)退行性疾病相關(guān)。近年來，研究者開發(fā)了新型基因編碼的NA熒光探針，通過實(shí)時(shí)監(jiān)測(cè)NA動(dòng)態(tài)變化，為研究NA相關(guān)神經(jīng)環(huán)路提供了重要工具。試劑盒檢測(cè)范圍為7.5 pg/mL-300 pg/mL，本試劑盒適用于神經(jīng)生物學(xué)研究、應(yīng)激反應(yīng)機(jī)制探索、心血管藥理學(xué)實(shí)驗(yàn)中對(duì)NA分泌水平的動(dòng)態(tài)監(jiān)測(cè)，也可用于評(píng)估抗高血壓藥物或神經(jīng)調(diào)節(jié)劑對(duì)NA代謝通路的影響。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    N/A
  • 縮寫：
    NA
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma
  • 檢測(cè)范圍：
    7.5 pg/mL-300 pg/mL
  • 靈敏度：
    7.5 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Neuroscience
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse noradrenaline in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 100  
    Range % 98-104  
    1:2 Average % 87  
    Range % 84-90  
    1:4 Average % 86  
    Range % 83-89  
    1:8 Average % 91  
    Range % 88-94  
  • 回收率：
    The recovery of mouse noradrenaline spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 85 82-89  
    EDTA plasma (n=4) 97 94-100  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    300 0.371 0.396 0.384    
    150 0.548 0.578 0.563    
    69 0.775 0.798 0.787    
    22.5 1.126 1.168 1.147    
    7.5 1.672 1.587 1.630    
    0 2.201 2.209 2.205    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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