Mouse Nuclear factor-kappa B,NF-κB ELISA Kit
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中文名稱:小鼠核因子κB(NF-κB)酶聯(lián)免疫試劑盒
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貨號(hào):CSB-E12108m
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規(guī)格:96T/48T
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價(jià)格:¥3800/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:小鼠核因子κB(NF-κB)酶聯(lián)免疫試劑盒(CSB-E12108m)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織培養(yǎng)上清液樣本中的NFKB1含量。NFKB1是一種轉(zhuǎn)錄因子,參與調(diào)節(jié)多種生物過程中的轉(zhuǎn)錄,與RELA/p65、RELB和NFKB2/p52形成復(fù)合物。其研究機(jī)制涉及基因表達(dá)變化,與應(yīng)激和免疫信號(hào)通路激活相關(guān),突變可能導(dǎo)致免疫缺陷。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL,適用于體外實(shí)驗(yàn)?zāi)P椭醒装Y信號(hào)通路機(jī)制研究、藥物作用靶點(diǎn)篩選或免疫調(diào)控實(shí)驗(yàn)的定量分析;適用于細(xì)胞刺激實(shí)驗(yàn)、動(dòng)物模型血清學(xué)分析及分子機(jī)制研究等科研場景,為生命科學(xué)領(lǐng)域提供穩(wěn)定可靠的NF - κB蛋白定量解決方案本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名:Nfkb1 ELISA Kit; Nuclear factor NF-kappa-B p105 subunit ELISA Kit; DNA-binding factor KBF1 ELISA Kit; EBP-1 ELISA Kit; NF-kappa-B1 p84/NF-kappa-B1 p98 ELISA Kit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1) [Cleaved into: Nuclear factor NF-kappa-B p50 subunit] ELISA Kit
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縮寫:
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Uniprot No.:
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種屬:Mus musculus (Mouse)
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樣本類型:serum, plasma, cell culture supernates
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檢測范圍:0.312 ng/mL-20 ng/mL
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靈敏度:0.078 ng/mL
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領(lǐng)域:Cell Biology
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse NF-κB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 92 Range % 86-99 1:2 Average % 97 Range % 92-103 1:4 Average % 86 Range % 80-89 1:8 Average % 90 Range % 83-99 -
回收率:
The recovery of mouse NF-κB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 95 89-99 EDTA plasma (n=4) 92 87-96 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average Corrected 20 2.176 2.237 2.207 2.004 10 1.635 1.642 1.639 1.436 5 1.184 1.201 1.193 0.990 2.5 0.795 0.813 0.804 0.601 1.25 0.601 0.637 0.619 0.416 0.625 0.435 0.446 0.441 0.238 0.312 0.333 0.341 0.337 0.134 0 0.201 0.204 0.203 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- Oral Administration of Ulva pertusa Kjellman Improves Intestinal Motility Against Loperamide-Induced Constipation in Mice EJ Koh, IY Sunwoo, YK Ryu, WK Lee, T Kim, WY Choi,Applied Sciences,2024
- L. carnitine can attenuate cadmium induced hepatotoxicity in rat: emphasis on TLR4-NFκB axis AA Elhemiely,Azhar International Journal of Pharmaceutical and Medical Sciences,2024
- Brown Seaweed Byproduct Extracts Improve Intestinal Motility and Auto-Inflammation in Mice with Loperamide-Induced Constipation EJ Koh,Foods,2024
- Licochalcone A plays dual antiviral roles by inhibiting RSV and protecting against host damage Z Li,Journal of medical virology,2023
- Phycocyanin stimulates ulcerative colitis healing via selective activation of cannabinoid receptor-2, intestinal mucosal healing, Treg accumulation, and p38MAPK/MK2 signaling inhibition WH El-Maadawy,Life sciences,2023
- Proanthocyanidins alleviate pentylenetetrazole-induced epileptic seizures in mice via the antioxidant activity NM Alyami,Neurochemical research,2023
- Protective potential of mokko lactone from Cheilocostus speciosus (J. Koenig) CD Specht (Costaceae) rhizomes against fulminant hepatic failure A ALTYAR,Turkish Journal of Botany,2023
- Alpha-Mangostin as a New Therapeutic Candidate for Concanavalin A-Induced Autoimmune Hepatitis: Impact on the SIRT1/Nrf2 and NF-κB Crosstalk AM Shehata,Plants,2022
- Alpha-Mangostin as a New Therapeutic Candidate for Concanavalin A-Induced Autoimmune Hepatitis: Impact on the SIRT1/Nrf2 and NF-κB Crosstalk AM Shehata,Plants,2022
- Vitamin D Combined with Pioglitazone Mitigates Type-2 Diabetes-induced Hepatic Injury Through Targeting Inflammation, Apoptosis, and Oxidative Stress HA Hamouda,Inflammation,2021
- Neuroprotective Effect of ADIOL against Lithium Pilocarpine Induced-Seizures, Behavioral Changes and Cognitive Deficits in Male Mice Heba Ahmed,Research gate,2019
- Hydroxytyrosol ameliorates oxidative challenge and inflammatory response associated with lipopolysaccharide-mediated sepsis in mice MA Alblihed,Human & Experimental Toxicology,2020
- Irbesartan mitigates acute liver injury, oxidative stress, and apoptosis induced by acetaminophen in mice Manar G. Helal,J Biochem Mol Toxicol,2020
- Protective role of pirfenidone against experimentally-induced pancreatitis Dalia H.El-Kashef, et al,Pharmacological Reports,2019
- OR-012 The Regulation of NF-κB-TNF-α/IDO/5-HT Axis by Aerobic Exercise against Hippocampal Neuroinflammation in CUMS Depressive Mice Honglin Qu, et al,Exercise Biochemistry Review,2018
- Perindopril, fosinopril and losartan inhibited the progression of diethylnitrosamine-induced hepatocellular carcinoma in mice via the inactivation of nuclear transcription factor kappa-B Sameh Saber.et al,Toxicology Letters,2018
- Nicorandil alleviates ovalbumin-induced airway inflammation in a mouse model of asthma Dalia H.El-Kashef.et al,Environmental Toxicology and Pharmacology,2018
- N-Acetyl-chitobiose ameliorates metabolism dysfunction through Erk/p38 MAPK and histone H3 phosphorylation in type 2 diabetes mice Xia Wu.et al,Journal of Functional Foods,2017
相關(guān)產(chǎn)品
相關(guān)問答
我想問一下CSB-E12108m小鼠細(xì)胞因子-kappa B (NF-κB) ELISA試劑盒的樣本處理方法。我有一個(gè)血清樣本,但在用于ELISA檢測之前,我會(huì)將其在56℃的溫度下加熱30分鐘以進(jìn)行熱滅活處理,這會(huì)影響我想檢測的細(xì)胞因子嗎?如果我的樣本經(jīng)過預(yù)加熱處理,靈敏度會(huì)如何?
靶點(diǎn)詳情
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功能:NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and RelB-p50 complexes are transcriptional activators. The NF-kappa-B p50-p50 homodimer is a transcriptional repressor, but can act as a transcriptional activator when associated with BCL3. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslational processing. The proteasome-mediated process ensures the production of both p50 and p105 and preserves their independent function, although processing of NFKB1/p105 also appears to occur post-translationally. p50 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. Plays a role in the regulation of apoptosis. Isoform 5, isoform 6 and isoform 7 act as inhibitors of transactivation of p50 NF-kappa-B subunit, probably by sequestering it in the cytoplasm. Isoform 3 (p98) (but not p84 or p105) acts as a transactivator of NF-kappa-B-regulated gene expression. In a complex with MAP3K8, NFKB1/p105 represses MAP3K8-induced MAPK signaling; active MAP3K8 is released by proteasome-dependent degradation of NFKB1/p105.
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基因功能參考文獻(xiàn):
- Reducing NF-kappaB activation attenuates PDLIM2 deficiency-exacerbated inflammation and dyslipidemia in Palmitate -treated primary hepatocytes. PMID: 29852170
- Chandipura virus infection triggered the activation of signalling pathways mediated by mitogen-activated protein kinases, including p38, JNK 1 and 2, and nuclear factor kappaB. PMID: 30001342
- These findings provide mechanistic insight into how polymorphisms that attenuate NFKB1 expression predispose humans to epithelial cancers, highlighting the pro-tumorigenic activity of STAT1 and identifying targetable vulnerabilities in GC. PMID: 29562203
- SIRT1 upregulation protects against liver injury induced by a HFD through inhibiting CD36 and the NF-kappaB pathway in mouse Kupffer cells. PMID: 29845302
- activation of NF-kappaB pathway is essential for Bacterial lipoprotein tolerance-augmented antimicrobial activity in innate phagocytes and depends primarily on both NOD1 and NOD2. PMID: 28079153
- High host NF-kappaB p50 induces murine glioblastoma tumor. PMID: 30030559
- NF-kappaB is responsible for Morgana dependent metastasis. PMID: 29158506
- study indicates that downregulation of NF-kappaB1 can suppress renal cell carcinoma tumorigenesis by inducing late apoptosis/necrosis PMID: 29212573
- TNF-alpha is involved in cardiac PHLPP1 upregulation during reoxygenation, which is mediated by NF-kappaB transcriptional activity PMID: 29940243
- Nuclear Factor kappa B (NF-kappaB) pathway in the bone marrow (BM) vascular niche is a critical signalling axis that regulates haematopoietic stem cells (HSCs) function. PMID: 28000664
- NF-kappaB and survivin are coordinately up-regulated in GBM patient tumors, and functional inhibition of either protein or BRD4 in in vitro and in vivo models restores sensitivity to EGFR tyrosine kinase inhibitors PMID: 28724615
- NF-kappaB1 phosphorylation is regulated by platelet-activating factor in macrophages. PMID: 27554194
- Blockade of CD38 diminishes lipopolysaccharide-induced macrophage classical activation and acute kidney injury involving NF-kappaB signaling suppression. PMID: 29080804
- NF-kappaBmiR15abFGF/VEGFA axis contributes to the impaired angiogenic capacity of bone marrowmesenchymal stem cells in high fat dietfed mice. PMID: 28944834
- Tyr42 phosphorylation of RhoA GTPase promotes tumorigenesis through NF-kappaB. PMID: 28712859
- By modulating the translation of IkappaBalpha via the Mnk2-eIF4E pathway, Brd4 provides an additional layer of control for NF-kappaB-dependent inflammatory gene expression and inflammatory response. PMID: 28461486
- BCA3 interacts with Rac1 and augments NF-kappaB signaling in vitro, but has no effect on RANKL-induced bone resorption in vivo. PMID: 28791343
- TLR4-NF-kappaB signaling pathway was involved in the lipopolysaccharide-induced acute lung injury. PMID: 28886315
- This is the first report demonstrating that NF-kappaB signaling may play a key role in compensatory lung growth (CLG). Given its pathway is crucial in tissue regeneration of various organs, NF-kappaB may shed light on identification of molecular triggers or clinically usable key regulators of CLG. PMID: 28679393
- Abrogating ClC-3 blunts lipopolysaccharide-induced Inflammation via blocking the TLR4/NF-kappaB pathway. PMID: 27363391
- miR-17 approximately 92 family clusters have a role in iNKT cell ontogenesis via modulation of TGF-beta signaling PMID: 27930306
- NF-kappaB1-mediated signaling regulates the development of gastric mucosal pathology following TAM administration. PMID: 28726772
- that TG2 depletion increases nuclear factor-kappaB (NF-kappaB) signaling PMID: 28522262
- LRRK1 physically interacted and potently synergized with CARMA1 to enhance NF-kappaB activation. PMID: 27166870
- NLRC5 knockout mice fed with high fat showed accelerated fibrosis and inflammation response by promoting alpha-SMA, Collagen I, Collagen III, TLR4/MyD88, phosphorylated IKKalpha, IkappaBalpha and NF-kappaB expression. PMID: 28499247
- Our data suggest that rCC16 suppresses LPS-mediated inflammatory mediator TNF-alpha, IL-6, and IL-8 production by inactivating NF-kappaB and p38 MAPK but not AP-1 in RAW264.7 cells. PMID: 28338974
- Our data indicate for the first time that the inflammasome is involved in the inflammatory response and cell death in hypoxia-induced beta cells through the ROS-TXNIP-NLRP3 axis in vitro. This provides new insight into the relationship between hypoxia and inflammation in T2D. PMID: 29278702
- Overall, these results suggest that p53 is involved in improving insulin sensitivity of hepatic cells via inhibition of mitogen-activated protein kinases (MAPKs) and NF-kappaB pathways. PMID: 29258820
- In conclusion, the results suggest that linarin has anti-osteoclastic effects and may serve as potential modulatory agents for the prevention and treatment of bone loss-associated diseases. PMID: 29269297
- The combination therapy of DANFIN with bortezomib dramatically enhanced the apoptosis of multiple myeloma cells and indicated a remarkable anti-tumor effect in a multiple-myeloma xenograft mouse model. PMID: 29284118
- A new mouse modelof CLL was created by crossing the Emicro-TCL1 mouse with the previously described p50 knockout mouse4 to study the role of p50 in CLL pathogenesis. The knockout animals had lower leukocyte counts and less disease burden than the wild type, implying a role for p50 in CLL. PMID: 28515090
- Results suggest that insufficient hepatocyte growth factor activator inhibitor type 1 (HAI-1) function promotes intestinal carcinogenesis by activating nuclear factor-kappaB (NF-kappaB) signaling. PMID: 27612426
- UVB-irradiated or aged mice skin revealed that mTORC2 activity was significantly upregulated which in turn increased Akt activation and Akt-dependent IkappaB kinase alpha (IKKalpha) phosphorylation, and The increased mTORC2 signaling pathway during skin aging were associated to NF-kappaB activation. PMID: 27486771
- activation of the NF-kappaB pathway through TNF plays an important role in the dedifferentiation of astrocytes via the re-expression of Oct4. PMID: 26381429
- The constitutive elevations of Prdx6 and NF-kappaB during Clonorchis sinensis infection may be associated with more severe persistent hepatobiliary abnormalities mediated by clonorchiasis. PMID: 27554973
- SPAK plays a pathogenic role in IgA nephropathy through the activation of NF-kappaB/MAPK signaling pathway. PMID: 27519267
- Identification of a PPAR-gamma --> NF-kappaB --> p22phox neuroprotective signaling cascade opens a new avenue for protecting the brain against ischemic insult. PMID: 26108185
- interactions of NF-kappaB and N-myc with GLT-1/EAAT2 promoter sequences was significantly elevated in the ipsi-lateral cortex of both adult and old Traumatic brain injury mice. PMID: 26081154
- Data show that inhibition of the protease activity of MALT1 might be a strategy to treat inflammatory bowel disease and the NLRP3 inflammasome and NF-kappaB activation are critical components in MALT1 signaling cascades in this disease model. PMID: 27105502
- IFN-gamma/TNFalpha trigger the formation of an NF-kappaB/STAT3 complex. PMID: 28264935
- Importantly, inhibition of NF-kappaB by QDs displayed promising effects against the viral replication and in vivo bacterial endotoxin-induced inflammatory responses. These data suggest the QDs as potent inhibitors of the NF-kappaB signaling pathway, both in vitro and in vivo. PMID: 27639114
- NF-kB mediated upregulation of CADM1 was identified as a mechanism of TNFalpha induced migration. PMID: 26867147
- NF-kappaB activation and the downstream expression of inflammatory factors were therefore down-regulated in along an efficient path and ameliorating the damage as a consequence of LPS-induced acute lung injury. PMID: 28778458
- FSTL1 is a secreted osteoclastogenic factor that plays a critical role in osteoclast formation via the NF-kappaB and MAPKs signaling pathways. PMID: 27234130
- Sustained NF-kappaB inhibition improves insulin sensitivity but is detrimental to muscle health. PMID: 28556540
- Prostaglandin E receptor subtype 2 (EP2; Ptger2) deficiency suppressed nuclear factor kappaB (NF-kappaB) activation in intracranial arteries 4 and 8 weeks after intracranial aneurysm induction. PMID: 28174280
- Loss of HDAC-mediated repression and gain of NF-kappaB activation underlie cytokine induction in ARID1A- and PIK3CA-mutation-driven ovarian cancer. PMID: 27681437
- Absence of Musashi2 in osteoclast precursors promotes apoptosis and inhibits RANKL-induced NF-kappaB activation, which is essential for osteoclast survival. PMID: 27441652
- Study demonstrated that activation of NF-kB in astrocytes contributes not only to white matter demyelination and axonal loss during chronic hypoxia but that an astrocytic pro-inflammatory pathway may have important consequences for cognitive outcome in neurodegenerative disease. PMID: 27487766
- NF-kappaB-mediated miR-130a modulation is critical in lung vascular remodeling. PMID: 28755990
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亞細(xì)胞定位:Nucleus. Cytoplasm. Note=Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B).; [Isoform 5]: Cytoplasm.; [Isoform 6]: Nucleus. Cytoplasm.; [Isoform 7]: Nucleus.
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