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Mouse Osteocrin(OSTN) ELISA kit

  • 中文名稱：
    小鼠骨織素(OSTN)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL017269MO
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠骨織素(OSTN)酶聯(lián)免疫試劑盒（CSB-EL017269MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的OSTN含量。OSTN即骨鈣素，是一種分泌型骨細胞因子。它在骨骼發(fā)育、礦化等過程中發(fā)揮重要作用。研究機制上，其能調(diào)節(jié)成骨細胞與破骨細胞的活動，影響骨代謝平衡，還可參與能量代謝、糖脂代謝等，有望成為骨代謝疾病等領域的治療靶點。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，適用于能量代謝研究、骨發(fā)育調(diào)控機制探索、運動生理學實驗等科研領域?？蓮V泛應用于肥胖模型、骨質(zhì)疏松癥研究或生物節(jié)律相關實驗中的OSTN動態(tài)監(jiān)測，為體外培養(yǎng)細胞或動物模型中的OSTN表達分析提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Ostn ELISA Kit; Osteocrin ELISA Kit; Musclin) [Cleaved into: Processed Osteocrin] ELISA Kit
  • 縮寫：
    OSTN
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    15.6 pg/mL-1000 pg/mL
  • 靈敏度：
    3.9 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Stem Cells
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse OSTN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:5Average %92
    Range %87-97
    1:10Average %95
    Range %88-99
    1:20Average %93
    Range %87-100
    1:40Average %95
    Range %87-104
  • 回收率：
    The recovery of mouse OSTN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9485-99
    EDTA plasma (n=4)9789-105
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    10002.579 2.605 2.592 2.502
    5001.447 1.494 1.471 1.381
    2500.791 0.803 0.797 0.707
    1250.430 0.427 0.429 0.339
    62.50.219 0.225 0.222 0.132
    31.20.144 0.149 0.147 0.057
    15.60.107 0.112 0.110 0.020
    00.088 0.091 0.090
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

相關問答

■ 常見問題解答
Q:

Is an extraction step required from Plasma?

A:
Thanks for your inquiry! Extraction step is not required from Plasma.

靶點詳情

  • 功能：
    Hormone that acts as a ligand for natriuretic peptide receptor NPR3/NPR-C and promotes bone growth and physical endurance in muscle. Acts as a regulator of osteoblast differentiation and bone growth by binding to natriuretic peptide receptor NPR3/NPR-C, thereby preventing binding between NPR3/NPR-C and natriuretic peptides, leading to increase cGMP production. Required to enhance physical endurance: induced following physical exercise in muscle and promotes cGMP production, probably by interacting with NPR3/NPR-C. May act as an autocrine and paracrine factor linked to glucose metabolism in skeletal muscle.
  • 基因功能參考文獻：
    1. The mechanism underlying the effect of OSTN on skeletal growth relevant to C-type natriuretic peptide /Natriuretic peptide receptor B signaling. PMID: 28990933
    2. Data show that musclin (Ostn) production is stimulated by Ca2+-dependent proto-oncogene protein c-Akt (Akt) phosphorylation. PMID: 26668395
    3. findings suggest that palmitate increases musclin gene expression via the activation of the PERK signaling pathway in C2C12 myotubes PMID: 26449458
    4. osteocrin represents a novel, unique vitamin D-regulated bone-specific protein that appears to act as a soluble osteoblast regulator PMID: 14523025
    5. Musclin expression level is tightly regulated by nutritional changes and its physiological role could be linked to glucose metabolism PMID: 15044443
    6. Foxo1 downregulates musclin mRNA expression both in vitro and in vivo, which should explain insulin-mediated upregulation of this gene in muscle cells. PMID: 17950246
    7. Ostn is a naturally occurring ligand of the NPR-C clearance receptor and may act to locally modulate the actions of the natriuretic system in bone by blocking the clearance action of NPR-C, thus locally elevating levels of C-type natriuretic peptide. PMID: 17951249

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Osteocrin family
  • 組織特異性：
    Expressed in skeletal muscle and to a much lesser extent in bone, brown adipose tissue, spleen and testis. Not expressed in neurons.
  • 數(shù)據(jù)庫鏈接：

    KEGG: mmu:239790

    UniGene: Mm.390880