Mouse Osteoprotegerin,OPG ELISA KIT
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中文名稱:小鼠骨保護(hù)素(OPG)酶聯(lián)免疫試劑盒
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貨號(hào):CSB-E04693m
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規(guī)格:96T/48T
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價(jià)格:¥3800/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:小鼠骨保護(hù)素(OPG)酶聯(lián)免疫試劑盒(CSB-E04693m)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的TNFRSF11B含量。TNFRSF11B是重要靶點(diǎn)。它參與調(diào)控骨代謝平衡,在破骨細(xì)胞生成和骨吸收過(guò)程中發(fā)揮關(guān)鍵作用。研究發(fā)現(xiàn)其異常與骨質(zhì)疏松等骨疾病密切相關(guān)。目前對(duì)它的研究機(jī)制主要聚焦于其信號(hào)通路,有望為相關(guān)疾病治療提供新思路。試劑盒檢測(cè)范圍為15.6 pg/mL-1000 pg/mL,靈敏度為3.9 pg/mL。廣泛應(yīng)用于骨代謝疾病模型構(gòu)建、藥物干預(yù)效果評(píng)估、骨骼發(fā)育研究及炎癥相關(guān)信號(hào)通路探索等科研場(chǎng)景,其穩(wěn)定性和特異性為體外實(shí)驗(yàn)提供可靠數(shù)據(jù)支持,是研究骨穩(wěn)態(tài)調(diào)控機(jī)制及病理過(guò)程的理想工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
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別名:Tnfrsf11b ELISA Kit; Ocif ELISA Kit; Opg ELISA Kit; Tumor necrosis factor receptor superfamily member 11B ELISA Kit; Osteoclastogenesis inhibitory factor ELISA Kit; Osteoprotegerin ELISA Kit
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縮寫(xiě):
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Uniprot No.:
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種屬:Mus musculus (Mouse)
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樣本類型:serum, plasma, tissue homogenates
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檢測(cè)范圍:15.6 pg/mL-1000 pg/mL
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靈敏度:3.9 pg/mL
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測(cè)波長(zhǎng):450 nm
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研究領(lǐng)域:Cell Biology
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測(cè)定原理:quantitative
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測(cè)定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse OPG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:5 Average % 87 Range % 83-90 1:10 Average % 102 Range % 98-106 1:20 Average % 85 Range % 81-89 1:40 Average % 97 Range % 93-100 -
回收率:
The recovery of mouse OPG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 85 81-89 EDTA plasma (n=4) 96 92-99 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 1000 2.045 2.102 2.074 1.938 500 1.357 1.308 1.333 1.197 250 0.879 0.915 0.897 0.761 125 0.593 0.578 0.586 0.450 62.5 0.381 0.368 0.375 0.239 31.2 0.275 0.298 0.287 0.151 15.6 0.193 0.187 0.190 0.054 0 0.135 0.137 0.136 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- Improvement of Menopausal Symptoms by Oat Hull Extracts in Ovariectomized Animal Model MJ Lee, Y Lee, HG Lee, H Jin, YA Shin, SY Song,Research Square,2024
- Ulmus macrocarpa Hance trunk bark extracts inhibit RANKL-induced osteoclast differentiation and prevent ovariectomy-induced osteoporosis in mice C Jeong,Journal of ethnopharmacology,2024
- Yak milk promotes renal calcium reabsorption in mice with osteoporosis via the regulation of TRPV5 J Zhang,Journal of dairy science,2023
- Regulation of bone and fat balance by Fructus Ligustri Lucidi in ovariectomized mice X Qin,Pharmaceutical biology,2023
- Effect of MC3T3 cell density on osteoclastic differentiation of mouse bone marrow cells Y Wang,Tissue Cell,2022
- Substance P blocks ovariectomy-induced bone loss by modulating inflammation and potentiating stem cell function J Piao,Aging-US,2020
- Melatonin prevents bone destruction in mice with retinoic acid-induced osteoporosis Wang X, et al,Molecular Medicine,2019
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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最新研究進(jìn)展:最近的研究表明,TNFRSF11B在骨代謝和腫瘤的發(fā)生中起著重要作用。
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功能:Acts as decoy receptor for TNFSF11/RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local ratio between TNFSF11 and TNFRSF11B. May also play a role in preventing arterial calcification. May act as decoy receptor for TNFSF10/TRAIL and protect against apoptosis. TNFSF10/TRAIL binding blocks the inhibition of osteoclastogenesis.
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基因功能參考文獻(xiàn):
- lactation increases physiological maxillary bone remodeling and orthodontic tooth movement, and both require activation of RANK/RANKL/OPG system PMID: 29382610
- Hdac3-depletion in osteoblasts increases expression of Opg, subsequently preserving insulin sensitivity. PMID: 28840938
- Epithelial rests of Malassez may be associated with root resorption via OPG, thus helping to explain the mechanism underlying root resorption. PMID: 29644561
- findings demonstrate that mTORC1 activation-stimulated RANKL expression in B cells is sufficient to induce bone loss and osteoporosis. The study also established a link between mTORC1 and the RANKL/OPG axis via negative regulation of beta-catenin. PMID: 26825871
- RANK/RANKL signaling is involved in the androgen deprivation therapy-induced acceleration of bone metastasis in castration-insensitive prostate cancer and is inhibited by osteoprotegerin to prevent bone metastasis. PMID: 28373003
- endocortical resorption is driven by reduced OPG rather than elevated RANKL expression PMID: 27460899
- Diabetes significantly increased OPG and the OPG/TRAIL ratio expression in the aorta, while dyslipidemia was the major determinant of the changes observed in the heart, where it significantly increased OPG and reduced TRAIL expression, thus increasing cardiac OPG/TRAIL ratio. PMID: 28070143
- These discoveries strongly suggest that Heparan Sulfate plays an active role in regulating OPG-RANKL interaction and osteoclastogenesis. PMID: 27697839
- OPG plays an important role in regulating bone marrow mesenchymal stem cells differentiation and bone marrow adipogenesis. PMID: 27898403
- These data suggested that OPG played an important role in maintaining the homeostasis of articular cartilage of femoral head. PMID: 27541035
- Data show that p38alpha mitogen-activated protein kinase (MAPK) in paired related homeobox 1 protein (Prx1+) bone marrow-derived mesenchymal stromal cells acts to preserve the bone by sustaining osteoprotegerin (OPG) production. PMID: 26947973
- OPG expression in chondrocyte increases bone mass in the proximal metaphysis of tibiae through negative regulation of osteoclast formation. PMID: 26329493
- the generation of Rag2GFP+ regulatory T cells is unaltered in Tnfrsf11b(-/-) mice PMID: 26254339
- identify osteoprotegerin as a HIF target gene capable of directing osteoblast-mediated osteoclastogenesis to regulate bone homeostasis PMID: 25846796
- Osteoprotegerin is increased in metabolic syndrome and promotes adipose tissue proinflammatory changes. PMID: 24998520
- These results indicate that OPG derived either from the bone marrow or from the vessel wall is sufficient to slow down lesion progression and vascular calcification independent of bone turnover. PMID: 23990207
- High concentrations of alendronate decrease the expressions of OPG and RANKL in cultured osteoblasts. PMID: 23268391
- In a xenograft model, increased osteoprotegerin expression correlated with a delay to pathologic fracture suggesting a potential role for mTOR inhibitors in the treatment of neuroblastoma bone metastases. PMID: 23331792
- Coptisine inhibits RANKL-induced NF-kappaB phosphorylation in osteoclast precursors and suppresses function through the regulation of RANKL and OPG gene expression in osteoblastic cells. PMID: 21656335
- OPG gene transfer to periodontal tissues could inhibit relapse after orthodontic tooth movement, through the inhibition of osteoclastogenesis PMID: 22196183
- Omentin-1 ameliorates arterial calcification and bone loss in vivo through the regulation of the RANK signalling pathway in osteoprotegerin-deficient mice. PMID: 21750093
- Increased bone resorption is a noncell autonomous defect, caused by exuberant stimulation of osteo-clastogenesis by Cx43-deficient bone marrow stromal cells, via decreased Opg production. PMID: 21346198
- Osteoprotegerin serum levels were decreased in all IL-6 deficient mice and in a highest degree in sham-operated hypothyroid mice. PMID: 21478097
- TRAIL and osteoprotegerin are expressed in atherosclerotic plaques in a calcification-prone mouse model PMID: 21426505
- RANKL and OPG could have a close relationship with the osteoclast activity during mandibular first molar eruption. PMID: 18788552
- distribution of the osteocytic lacunar-canalicular system and osteocyte-secreting molecules--dentin matrix protein 1 and sclerostin--in the epiphyses and cortical bones in osteoprotegerin deficiency PMID: 21079361
- overexpression in preosteoblasts promotes their differentiation into mature osteoblasts PMID: 20936961
- Co2+ and Cr3+ can stimulate the mRNA expressions of RANKL and osteoprotegerin in osteoblasts. PMID: 20369527
- mechanical stretching may play an important role in bone remodeling through the upregulation of OPG and the mechanical signaling leading to OPG induction involves the noncanonical Wnt pathway. PMID: 20200998
- Osteoprotegerin(OPG) ex vivo gene therapy may be useful to treat patients for aseptic loosening of prosthetic implants. PMID: 11918293
- expression of RANKL and osteoprotegerin mRNAs by marrow stromal cells PMID: 12211438
- Osteosclerosis in mice with thrombopoietin overexpression occurs predominantly via up-regulation of osteoprotegerin in host stromal cells leading to disruption of osteoclastogenesis. PMID: 12506018
- The presence of OPG on the membrane of these cells was confirmed by western blotting and immunostaining, sRANKL was found to be bound to the OPG on the osteoclast precursors. PMID: 12526098
- expression of RANKL and OPG change with age in whole bone and in cultured osteoblast-like cells PMID: 12568404
- Runx2 promotes osteoclast differentiation by inducing RANKL and inhibiting OPG PMID: 12697767
- osteoprotegerin and RANK ligand have roles in breast cancer bone metastasis PMID: 12923331
- bone formation is coupled with bone resorption at local sites in OPG-/- mice, and that serum RANKL levels do not reflect this coupling. PMID: 14500574
- OPG secretion is regulated through calmodulin-sensitive protein kinase signaling that depends on the activity of the L-type VSCC and is mediated through the cAMP response element-binding protein. PMID: 14525906
- suppression of gene expression affects osteoclastogenic activity of parathyroid hormone PMID: 14969393
- Expression in mice with collagen-induced arthritis treated with Kanzo-bushi-to, a Kampo medicine. PMID: 15340228
- expression of RANKL and OPG and the formation of osteoclast-like cells do not require Runx2 PMID: 15474477
- RANKL inhibitor osteoprotegerin is used to evaluate the role of osteoclast-mediated hypercalcemia in two murine models of hypercalcemia of malignancy. PMID: 15845617
- Microorganism-reactive CD4+ T-cells and the RANKL-osteoprotegerin axis provide the molecular basis of the advanced periodontal breakdown in diabetes. PMID: 15855336
- findings in OPG knockout mice support the hypothesis that OPG is important in the inhibition of bone remodeling within the otic capsule and the maintenance of normal auditory function PMID: 16467704
- Glucocorticoids regulate osteoclast differentiation and expression of receptor activator of nuclear factor-kappaB (NF-kappaB) ligand, osteoprotegerin, and receptor activator of NF-kappaB in mouse calvarial bones PMID: 16614077
- OPG inhibits advanced plaque progression by preventing an increase in lesion size and lesion calcification. OPG may act as a survival factor and may modulate MMP9 production in vascular cells. PMID: 16840715
- microgravity is capable to indirectly stimulate osteoclast formation and activity by regulating osteoblast secretion of crucial regulatory factors such as RANKL and OPG PMID: 16927271
- Altogether, these findings suggest that increased OPG production represents an early event in the natural history of diabetes mellitus, possibly contributing to disease-associated endothelial cell dysfunction. PMID: 17148684
- OPG suppresses the shedding of RANKL from osteoblasts and the serum RANKL in OPG(-/-) mice exactly reflects the state of bone resorption. PMID: 17182555
- bone resorptive action of Dexamethasone is mediated mainly by the inhibition of OPG by transrepressing the OPG gene through the AP-1 site PMID: 17516544
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亞細(xì)胞定位:Secreted.
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組織特異性:Highly expressed in liver, lung, stomach, intestines and calvaria. Highly expressed in decidua and placenta, and in embryo.
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