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中文名稱:小鼠過氧化物氧化還原酶-1(PRDX1)酶聯(lián)免疫試劑盒
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貨號:CSB-EL018653MO
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規(guī)格:96T/48T
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價格:¥3600/¥2500
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其他:
產品詳情
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產品描述:小鼠過氧化物氧化還原酶-1(PRDX1)酶聯(lián)免疫試劑盒(CSB-EL018653MO)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的PRDX1含量。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL,該產品適用于研究氧化應激相關疾?。ㄈ缟窠浲诵行约膊 ⒋x綜合征或炎癥模型)中PRDX1的動態(tài)變化,也可用于評估抗氧化藥物或基因干預手段對PRDX1表達調控的效果,為探索氧化還原平衡機制提供可靠的實驗工具。本品僅用于科研,不用于臨床診斷,產品具體參數(shù)及操作步驟詳見產品說明書。
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別名:Prdx1 ELISA Kit; Msp23 ELISA Kit; Paga ELISA Kit; Tdpx2 ELISA Kit; Peroxiredoxin-1 ELISA Kit; EC 1.11.1.15 ELISA Kit; Macrophage 23 kDa stress protein ELISA Kit; Osteoblast-specific factor 3 ELISA Kit; OSF-3 ELISA Kit; Thioredoxin peroxidase 2 ELISA Kit; Thioredoxin-dependent peroxide reductase 2 ELISA Kit
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縮寫:
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Uniprot No.:
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種屬:Mus musculus (Mouse)
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樣本類型:serum, plasma, tissue homogenates
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檢測范圍:0.312 ng/mL-20 ng/mL
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靈敏度:0.078 ng/mL
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反應時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領域:Cell Biology
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse PRDX1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 90 Range % 86-94 1:2 Average % 98 Range % 92-104 1:4 Average % 102 Range % 98-106 1:8 Average % 94 Range % 90-98 -
回收率:
The recovery of mouse PRDX1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 97 93-101 EDTA plasma (n=4) 94 91-97 -
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average Corrected 20 2.788 2.652 2.720 2.539 10 1.764 1.693 1.729 1.548 5 1.110 1.063 1.087 0.906 2.5 0.721 0.689 0.705 0.524 1.25 0.366 0.387 0.377 0.196 0.625 0.293 0.276 0.285 0.104 0.312 0.229 0.235 0.232 0.051 0 0.179 0.182 0.181 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻
- Extracellular Prdx1 mediates bacterial infection and inflammatory bone diseases JH Kang,Life sciences,2023
- Circulating Peroxiredoxin-1 is a novel damage-associated molecular pattern and aggravates acute liver injury via promoting inflammation He Y, et al,Free Radical Biology and Medicine,2019
相關產品
靶點詳情
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功能:Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation.
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基因功能參考文獻:
- Data indicate the basic mechanisms connecting peroxiredoxin 1 (PRDX1) and progesterone production in the functional stage of corpus luteum (CL). PMID: 30111318
- These phenomena increased liver apoptosis through increasing cleaved caspase3 protein expression in Prx I/ mice after LPS injections, resulting in high lethality after LPS challenges. These findings provide a new insight for understanding the function of Prx I against endotoxininduced injury. PMID: 29901182
- Data suggests that Prdx1 and Prdx2 in pronuclei of zygotes are involved in antioxidant mechanisms and epigenetic programming during maternal-to-zygotic transition (during ectogenesis following in vitro fertilization). PRDX1 and PRDX2 appear to be ninth and twenty-second highest expressed proteins, respectively, among 64 proteins identified in mouse zygotes. PMID: 29503398
- Prdx1 knockout can aggravate the oxidative stress and lung injury by increasing the level of Reactive Oxygen Species (ROS), and also activate P38/JNK signaling pathway. PMID: 28485790
- validated as novel and reliable, tissue-specific reference gene in qRT-PCR studies of postnatal mammary gland development PMID: 27752147
- PRX1 and PRX5 were upregulated in osteoblasts in the proximal tibial metaphysis of ovariectomized mice. Interestingly, PRX1 and PRX5 showed different distribution patterns, with PRX1 mainly accumulated in cell nuclei and PRX5 in the cytoplasm. PMID: 27786251
- HDAC6 inhibition and the regulation of Prx1 activity may play a significant role in protecting retinal cells and in particular photoreceptors. PMID: 29048427
- This is the first report proclaiming that the ESAT-6 regulates Prdx-1 which is involved in the increase of mycobacterial uptake and survival. The intermediate mechanisms involve the increased Prdx-1 production in macrophages through the activation of p38 and NRF-2 dependent signaling. PMID: 29032183
- in the absence of PAG, Csk becomes more associated with alternative partners; i.e., phosphatase PTPN22 and Dok adaptors. Combining PAG deficiency with PTPN22 or Dok adaptor deficiency further enhances effector T cell responses. Unlike PAG, Cbl ubiquitin ligases inhibit the activation of naive, but not of effector, T cells. PMID: 27926878
- This study demonstrated that Prdx1 showed a distinct nuclear localization in the spinal cord also present in the nucleus of dorsal root ganglia (DRG) cells as well as in the sensory nerve root in embryonic development. PMID: 25975898
- Prx I and Prx II appear to have a tight association with the mechanism underlying the protection of ESC stemness in developing teratomas. PMID: 24715692
- Prx I is an antioxidant that is up-regulated in a reactive oxygen species/p38 MAPK-dependent manner and governs the progression of neuroinflammation by suppressing microglial activation. PMID: 23602274
- NO/SNO donors such as S-nitrosocysteine and S-nitrosoglutathione readily induced the S-nitrosylation of Prx1, causing structural and functional alterations PMID: 23479738
- PrxI is not an effective protector against ozone-induced oxidative damage but plays a positive role in the initiation of lung inflammation following exposure. PMID: 23019345
- Peroxiredoxin 1 stimulates endothelial cell expression of VEGF via TLR4 dependent activation of HIF-1alpha. PMID: 23185615
- The clearance rate of Heinz body-containing RBCs in peroxiredoxin I(-/-) mice decreased significantly through the treatment of aniline hydrochloride (AH) compared with wild-type mice. PMID: 23101509
- Yiqi chutan recipe can inhibit the growth and metastasis of Lewis lung carcinoma and reduce expression of PRDX1 and PRDX6. PMID: 21434354
- up-regulation of Prx I gene expression by LPS is part of the cellular response to stress and may protect against oxidative stress-related injury in RAW 264.7 cells PMID: 19941984
- The localized inactivation of PrxI allows for the transient accumulation of H(2)O(2) around membranes, where signaling components are concentrated, while preventing the toxic accumulation of H(2)O(2) elsewhere PMID: 20178744
- incubation of Prx1 with thioglycollate-elicited murine macrophages or immature bone marrow-derived dendritic cells resulted in TLR4-dependent secretion of TNF-alpha and IL-6 and dendritic cell maturation PMID: 20018613
- Pathways of induction of peroxiredoxin I expression in osteoblasts: roles of p38 mitogen-activated protein kinase and protein kinase C. PMID: 11796722
- induced by oxidative and nitrosative stress in pancreatic beta cells PMID: 12107731
- a putative tumor suppressor which interacts with the Myc Box II domain of c-Myc and selectively alters its biological function and target gene expression PMID: 12196529
- results show that the sulfinic form of peroxiredoxin I, produced during the exposure of cells to H2O2, is rapidly reduced to the catalytically active thiol form PMID: 12714748
- mice lacking Prdx1 are viable and fertile but have a shortened lifespan owing to the development beginning at about 9 months of severe haemolytic anaemia and several malignant cancers PMID: 12891360
- peroxiredoxin I gene expression is activated via a protein kinase C, Ras, p38 mitogen-activated protein kinase signaling pathway PMID: 12960165
- Mouse peroxiredoxin I can be successfully expressed in IEC-6 cells. PMID: 15237445
- mammalian sulfiredoxin reactivates hyperoxidized peroxiredoxin through reduction of cysteine sulfinic acid in the active site to cysteine PMID: 15448164
- increase in NFkappaB activation and reactive oxygen species (ROS) levels in the cytoplasm as a result of Prdx1 knockdown PMID: 15858817
- Prdx1 regulates reactive oxygen species, DNA damage, and c-Myc function. PMID: 16170382
- Prdx1 is activated by histone deacetylase inhibitor FK228, which causes apoptosis induction in esophageal cancer cells PMID: 16278420
- multiple roles for Prx I in macrophage-derived foam cells that include functionality as both an antioxidant and a regulator of oxidant-sensitive signal transduction. PMID: 16880205
- eEF1A-2 interacts with Prdx-I to functionally provide cells with extraordinary resistance to oxidative stress-induced cell death[eEF1A-2 and Prdx-I] PMID: 16888816
- Expression of 40 proteins, including the antioxidative enzymes peroxiredoxin 1 and 3, glutathione peroxidase 1, and SOD-1, was increased with diabetes, suggesting an adaptive response to oxidative stress associated with this diabetic model. PMID: 17609286
- endogenously produced NO participates in protection of murine primary macrophages against oxidative and nitrosative stress by inducing Prx I and VI expression at mRNA and protein levels. PMID: 17921138
- LPS-dependent inhibition of Prx I gene activation by TPA in monocytes is regulated via a pathway that involves phosphorylation of the NF-kappaB subunit p65 at serine 276. PMID: 18070609
- In a spontaneous model of atherosclerosis, the antioxidant enzyme Prdx1 is shown to protect against endothelial activation and inflammation. PMID: 18689572
- The authors found binding of the peroxidase Prdx1 to PTEN essential for protecting PTEN from oxidation-induced inactivation. PMID: 19369943
- Study identified the antioxidant enzyme Prdx1 as an interactor of GDE2 and uncover a developmental role for Prdx1 in regulating neuronal differentiation in the spinal cord. PMID: 19766572
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亞細胞定位:Cytoplasm.
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蛋白家族:Peroxiredoxin family, AhpC/Prx1 subfamily
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組織特異性:Found in various tissues; high concentration in liver.
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數(shù)據(jù)庫鏈接:
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