Mouse Phospholipid hydroperoxide glutathione peroxidase, mitochondrial(GPX4) ELISA kit

產(chǎn)品詳情

文獻(xiàn)引用 (2) 說(shuō)明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

小鼠谷胱甘肽過(guò)氧化物酶，線粒體(GPX4)酶聯(lián)免疫試劑盒（CSB-EL009869MO）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的GPX4含量。GPX4是鐵死亡過(guò)程中的關(guān)鍵調(diào)節(jié)因子，通過(guò)將GSH轉(zhuǎn)化為GSSH來(lái)限制脂質(zhì)過(guò)氧化，保護(hù)細(xì)胞免受鐵死亡。其表達(dá)受NRF2調(diào)控，在多種腫瘤治療中具有潛在應(yīng)用價(jià)值。試劑盒檢測(cè)范圍為31.25 pg/mL-2000 pg/mL，適用于科研領(lǐng)域中對(duì)GPX4蛋白表達(dá)的動(dòng)態(tài)監(jiān)測(cè)，如鐵死亡機(jī)制研究、抗氧化藥物篩選、疾病模型構(gòu)建及相關(guān)信號(hào)通路探索。為細(xì)胞生物學(xué)、基礎(chǔ)醫(yī)學(xué)研究提供靈敏穩(wěn)定的分析工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
縮寫：

GPX4
Uniprot No.：

Q91XR9
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測(cè)范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.81 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Cancer
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse GPX4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	86-95
1:2	Average %	100
	Range %	97-104
1:4	Average %	94
	Range %	85-97
1:8	Average %	98
	Range %	91-103

回收率：

The recovery of mouse GPX4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	92	87-98

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.