Mouse Platelet-derived growth factor subunit B(PDGFB) ELISA kit
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中文名稱:小鼠血小板衍生生長(zhǎng)因子β(PDGFB)酶聯(lián)免疫試劑盒
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貨號(hào):CSB-EL017709MO
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規(guī)格:96T/48T
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價(jià)格:¥3600/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:小鼠血小板衍生生長(zhǎng)因子β(PDGFB)酶聯(lián)免疫試劑盒(CSB-EL017709MO)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的PDGFB含量。PDGFB 即血小板衍生生長(zhǎng)因子 B,在細(xì)胞生長(zhǎng)、增殖和分化等過(guò)程中扮演關(guān)鍵角色。其異常表達(dá)與腫瘤生長(zhǎng)、纖維化疾病等密切相關(guān)。研究機(jī)制主要聚焦于其與受體結(jié)合后激活下游信號(hào)通路,調(diào)控細(xì)胞行為,為相關(guān)疾病治療提供靶點(diǎn)。試劑盒檢測(cè)范圍為93.75 pg/mL-6000 pg/mL,其動(dòng)態(tài)監(jiān)測(cè)可為心血管疾病、纖維化疾病及腫瘤機(jī)制研究提供重要數(shù)據(jù)支持;適用于體外實(shí)驗(yàn)中PDGFB的表達(dá)水平分析,例如細(xì)胞因子調(diào)控研究、疾病模型病理分析或藥物干預(yù)效果評(píng)估等科研場(chǎng)景;可為細(xì)胞實(shí)驗(yàn)、動(dòng)物模型及離體組織研究提供穩(wěn)定可靠的定量檢測(cè)工具,助力科研人員探索PDGFB在生物學(xué)過(guò)程中的分子機(jī)制。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
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縮寫(xiě):
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種屬:Mus musculus (Mouse)
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樣本類型:serum, plasma, cell culture supernates, tissue homogenates
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檢測(cè)范圍:93.75 pg/mL-6000 pg/mL
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靈敏度:23.44 pg/mL
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測(cè)波長(zhǎng):450 nm
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研究領(lǐng)域:Cardiovascular
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測(cè)定原理:quantitative
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測(cè)定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
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線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse PDGFB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
86
Range %
82-91
1:2
Average %
88
Range %
83-93
1:4
Average %
92
Range %
85-99
1:8
Average %
85
Range %
81-90
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回收率:
The recovery of mouse PDGFB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
85
81-90
EDTA plasma (n=4)
90
83-97
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標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml
OD1
OD2
Average
Corrected
6000
2.090
2.034
2.062
1.901
3000
1.308
1.227
1.267
1.106
1500
0.864
0.828
0.846
0.685
750
0.526
0.519
0.523
0.361
375
0.334
0.321
0.327
0.166
187.5
0.243
0.253
0.248
0.087
93.75
0.201
0.194
0.198
0.036
0
0.158
0.165
0.161
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數(shù)據(jù)處理:
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貨期:3-5 working days
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