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  4. Mouse Protein FAM132B(FAM132B) ELISA kit

Mouse Protein FAM132B(FAM132B) ELISA kit

  • 中文名稱：
    小鼠FAM132B蛋白(FAM132B)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL008059MO
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠FAM132B蛋白(FAM132B)酶聯(lián)免疫試劑盒（CSB-EL008059MO）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的FAM132B含量。試劑盒檢測(cè)范圍為18.75 pg/mL-1200 pg/mL，適用于探究FAM132B在代謝性疾病模型、基因敲除/過(guò)表達(dá)實(shí)驗(yàn)中的動(dòng)態(tài)表達(dá)特征，或用于評(píng)估特定干預(yù)措施對(duì)FAM132B調(diào)控通路的影響，為代謝調(diào)控機(jī)制、細(xì)胞應(yīng)激反應(yīng)等基礎(chǔ)研究提供可靠數(shù)據(jù)支持本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    Erfe ELISA Kit; C1qtnf15 ELISA Kit; Ctrp15 ELISA Kit; Fam132bErythroferrone ELISA Kit; Complement C1q tumor necrosis factor-related protein 15 ELISA Kit; Myonectin ELISA Kit
  • 縮寫(xiě)：
    FAM132B
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    18.75 pg/mL-1200 pg/mL
  • 靈敏度：
    4.68 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Cell Biology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse FAM132B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %85
    Range %80-90
    1:2Average %93
    Range %87-99
    1:4Average %102
    Range %98-107
    1:8Average %98
    Range %94-102
  • 回收率：
    The recovery of mouse FAM132B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9589-101
    EDTA plasma (n=4)8984-94
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    12002.388 2.533 2.461 2.297
    6001.560 1.588 1.574 1.410
    3000.978 0.901 0.940 0.776
    1500.622 0.602 0.612 0.448
    750.363 0.355 0.359 0.195
    37.50.274 0.295 0.285 0.121
    18.750.189 0.202 0.196 0.032
    00.159 0.168 0.164
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Iron-regulatory hormone that acts as an erythroid regulator after hemorrhage: produced by erythroblasts following blood loss and mediates suppression of hepcidin (HAMP) expression in the liver, thereby promoting increased iron absorption and mobilization from stores. Promotes lipid uptake into adipocytes and hepatocytes via transcriptional up-regulation of genes involved in fatty acid uptake.
  • 基因功能參考文獻(xiàn)：
    1. Erfe(-/-) mice failed to adequately suppress hepcidin expression after infection with Plasmodium berghei K173. Importantly, the sustained production of hepcidin allowed by erythroferrone ablation was associated with decreased parasitemia, providing further evidence that transient iron restriction could be beneficial in the treatment of malaria. PMID: 27658439
    2. The here presented data unearthed EPO-dependent erythroferrone expression acts as an erythropoiesis-driven regulator of iron metabolism under phenylhydrazine-induced hemolytic anemia. PMID: 27067488
    3. Erythroferrone contributes to hepcidin suppression and iron overload in a mouse model of beta-thalassemia. PMID: 26276665
    4. Compared with wild-type mice, Fam132b-deficient mice exhibited a more severe anemia, had higher hepcidin levels and consequently lower serum iron concentration on days 14 and 21, and manifested impaired mobilization of iron from stores (liver and spleen). PMID: 25193872
    5. myonectin is a novel regulator of cellular autophagy PMID: 24187137

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Adipolin/erythroferrone family
  • 組織特異性：
    Predominantly expressed in skeletal muscle and, at much lower levels, in other tissues, including lung, eye, smooth muscle, heart, brain and kidney. Within skeletal muscles, higher expression levels in soleus as compared with plantaris. Found in blood (at
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