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  4. Mouse Protein NOV homolog(NOV) ELISA kit

Mouse Protein NOV homolog(NOV) ELISA kit

  • 中文名稱：
    小鼠NOV蛋白同源物(NOV)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL015956MO
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠NOV蛋白同源物(NOV)酶聯(lián)免疫試劑盒（CSB-EL015956MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的NOV含量。試劑盒檢測范圍為23.5 pg/mL-1500 pg/mL，可應(yīng)用于小鼠模型中的NOV蛋白表達水平研究，例如在組織再生、心血管疾病或代謝性疾病相關(guān)機制探索中，評估不同處理條件或病理狀態(tài)下蛋白的動態(tài)變化，為分子機制研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Ccn3 ELISA Kit; NovCCN family member 3 ELISA Kit; Cellular communication network factor 3 ELISA Kit; Nephroblastoma-overexpressed gene protein homolog ELISA Kit; Protein NOV homolog ELISA Kit; NovH ELISA Kit
  • 縮寫：
    NOV
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    23.5 pg/mL-1500 pg/mL
  • 靈敏度：
    5.85 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse NOV in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1000 Average % 87
    Range % 82-91
    1:2000 Average % 96
    Range % 92-100
    1:4000 Average % 91
    Range % 86-95
    1:8000 Average % 104
    Range % 100-112
  • 回收率：
    The recovery of mouse NOV spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 92 88-96
    EDTA plasma (n=4) 84 80-88
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    1500 2.737 2.623 2.680 2.590
    750 1.841 1.715 1.778 1.688
    375 1.060 1.004 1.032 0.942
    187.5 0.683 0.650 0.667 0.577
    94 0.363 0.371 0.367 0.277
    47 0.244 0.259 0.252 0.162
    23.5 0.171 0.162 0.167 0.077
    0 0.093 0.087 0.090 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Immediate-early protein playing a role in various cellular processes including proliferation, adhesion, migration, differentiation and survival. Acts by binding to integrins or membrane receptors such as NOTCH1. Essential regulator of hematopoietic stem and progenitor cell function. Inhibits myogenic differentiation through the activation of Notch-signaling pathway. Inhibits vascular smooth muscle cells proliferation by increasing expression of cell-cycle regulators such as CDKN2B or CDKN1A independently of TGFB1 signaling. Ligand of integrins ITGAV:ITGB3 and ITGA5:ITGB1, acts directly upon endothelial cells to stimulate pro-angiogenic activities and induces angiogenesis. In endothelial cells, supports cell adhesion, induces directed cell migration (chemotaxis) and promotes cell survival. Plays also a role in cutaneous wound healing acting as integrin receptor ligand. Supports skin fibroblast adhesion through ITGA5:ITGB1 and ITGA6:ITGB1 and induces fibroblast chemotaxis through ITGAV:ITGB5. Seems to enhance bFGF-induced DNA synthesis in fibroblasts. Involved in bone regeneration as a negative regulator. Enhances the articular chondrocytic phenotype, whereas it repressed the one representing endochondral ossification. Impairs pancreatic beta-cell function, inhibits beta-cell proliferation and insulin secretion. Plays a role as negative regulator of endothelial pro-inflammatory activation reducing monocyte adhesion, its anti-inflammatory effects occur secondary to the inhibition of NF-kappaB signaling pathway. Contributes to the control and coordination of inflammatory processes in atherosclerosis. Attenuates inflammatory pain through regulation of IL1B- and TNF-induced MMP9, MMP2 and CCL2 expression. Inhibits MMP9 expression through ITGB1 engagement.
  • 基因功能參考文獻：
    1. Taken together, our results suggest that palmitoylation by ZDHHC22 at C241 in the CCN3 TSP1 domain may be required for the secretion of CCN3. Aberrant palmitoylation induces intracellular accumulation of CCN3, inhibiting neuronal axon growth. PMID: 29287726
    2. Administration of EET alters Wnt1, NOV, and HO-1 signaling to prevent obesity-induced cardiomyopathy in obese mice. PMID: 28576832
    3. results show that NOV is a new adipocytokine that could be involved in obesity-associated insulin-resistance. PMID: 27284105
    4. Genetic and pharmacological approaches implicated the ERK1/2 pathway as a critical regulator of CCN3-dependent AAA development. PMID: 26974158
    5. Reduced NOV expression limits inflammation and interstitial renal fibrosis after obstructive nephropathy in mice. PMID: 26367310
    6. NOV is required for the maintenance of articular cartilage and for joint homeostasis, with disruption of NOV in ageing Nov(del3)-/- male mice causing osteoarthritis-like disease. PMID: 25541297
    7. tight regulation of CCN3 expression is necessary for normal cortical neuronal connectivity during development, and RAB25 negatively regulates neuronal differentiation as a downstream effector of CCN3 PMID: 25871796
    8. CCN3 overexpression is associated with control of inflammatory processes and reversion of dyslipidemia in the process of atherosclerosis, which implies that CCN3 may be a promising target in the treatment of atherosclerosis. PMID: 24722330
    9. in obese humans and mice plasma NOV levels positively correlated with NOV expression in adipose tissue, and support a possible contribution of NOV to obesity-related inflammation. PMID: 23785511
    10. CCN3 is up-regulated in the early phase of bone regeneration and acts as a negative regulator for bone regeneration. PMID: 23653360
    11. CCN3 upregulates RANK expression in murine osteoclast precursors in RAW 264.7 cells to promote RANKL-dependent osteoclastogenesis. PMID: 23536580
    12. Expression of NOV and BNIP3 in leukemia AML-M(4) is significantly higher than that in normal controls. PMID: 21518474
    13. Nov stabilizes gremlin transcripts, and this effect is possibly mediated by AU-rich elements present in the 3'-UTR of gremlin. PMID: 21268093
    14. Ccn3/Nov is directly induced by cytokines through STAT5A/B. PMID: 20720003
    15. CCN3 counter-regulates positive signals from TGF-beta and Wnt for fibrillin fibrillogenesis and profibrotic gene expression. PMID: 20182440
    16. CCN3 suppresses neointimal thickening through the inhibition of vascular smooth cell migration and proliferation. PMID: 20139355
    17. Nov sensitizes osteoblasts to BMP-2, but Nov is dispensable for the maintenance of bone mass. PMID: 19934377
    18. CCN3 binds to the C terminus of Cx43 and has a role in mediating growth control induced by specific gap junction proteins PMID: 15213231
    19. Study indicates that NOV is a positive modulator of chondrogenesis. PMID: 16294274
    20. Taken together, these results identify NOV as an adhesion substrate for myoblasts which, in concert with growth factors, could play a role in the physiology of muscle cells. PMID: 16371345
    21. Results suggest that NOV (nephroblastoma overexpressed) is a specific cell fate regulator in the myogenic lineage, acting negatively on key myogenic genes thus controlling the transition from progenitor cells to myoblasts. PMID: 16600215
    22. Exerts inhibitory effects on bone morphogenetic protein-2(BMP-2)-induced osteoblast differentiation by its involvement of the BMP and Notch signaling pathways. PMID: 17250806
    23. Nov binds BMP-2 and antagonizes BMP-2 and Wnt activity, and its overexpression inhibits osteoblastogenesis and causes osteopenia. PMID: 17500060
    24. NOV is a regulator of skeletal and cardiac development, and implicates NOV in various disease processes including cardiomyopathy, muscle atrophy and cataract formation. PMID: 18289368
    25. CCN2 induces chondrocyte differentiation by regulating the expression of chondrocyte-associated genes but that these effects are counteracted by CCN3. PMID: 18597638

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  • 亞細胞定位：
    Secreted. Cytoplasm. Cell junction, gap junction.
  • 蛋白家族：
    CCN family
  • 組織特異性：
    Expressed in large vessels including the ascending aorta, carotid arteries, and the thoracic aorta, in medium-sized vessels such as coronary arteries and small pulmonary veins and also in small vessels. In addition, also found to be present in the heart (
  • 數(shù)據(jù)庫鏈接：

    KEGG: mmu:18133

    STRING: 10090.ENSMUSP00000054389

    UniGene: Mm.5167