Mouse Protein Wnt-7a(WNT7A) ELISA kit
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中文名稱:小鼠Wnt-7a蛋白(WNT7A)酶聯(lián)免疫試劑盒
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貨號(hào):CSB-EL026141MO
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規(guī)格:96T/48T
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價(jià)格:¥3600/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:小鼠Wnt-7a蛋白(WNT7A)酶聯(lián)免疫試劑盒(CSB-EL026141MO)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的WNT7A含量。WNT7A是一種參與神經(jīng)發(fā)生、血管生成和血腦屏障維持的重要蛋白。其研究機(jī)制涉及通過調(diào)節(jié)細(xì)胞周期、神經(jīng)元分化和突觸形成等過程,對(duì)神經(jīng)發(fā)生的多個(gè)步驟進(jìn)行關(guān)鍵控制。此外,WNT7A在疾病如神經(jīng)退行性疾病、癌癥和角膜損傷修復(fù)中發(fā)揮重要作用。試劑盒檢測(cè)范圍為0.156 ng/mL-10 ng/mL,適用于基礎(chǔ)研究中對(duì)Wnt - 7a功能的探索,如發(fā)育生物學(xué)中組織特異性表達(dá)分析、骨骼疾病或神經(jīng)系統(tǒng)病變機(jī)制研究,以及靶向Wnt通路的藥物干預(yù)效果評(píng)估。其兼容性設(shè)計(jì)支持多種生物樣本的并行檢測(cè),為科研人員在細(xì)胞模型、動(dòng)物模型及離體組織實(shí)驗(yàn)中定量追蹤Wnt - 7a動(dòng)態(tài)變化提供可靠工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名:Wnt7a ELISA Kit; Wnt-7a ELISA Kit; Protein Wnt-7a ELISA Kit
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縮寫:
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Uniprot No.:
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種屬:Mus musculus (Mouse)
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樣本類型:serum, plasma, tissue homogenates, cell lysates
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檢測(cè)范圍:0.156 ng/mL-10 ng/mL
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靈敏度:0.039 ng/mL
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測(cè)波長(zhǎng):450 nm
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研究領(lǐng)域:Stem Cells
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測(cè)定原理:quantitative
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測(cè)定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
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線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse WNT7A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
93
Range %
86-100
1:2
Average %
98
Range %
91-105
1:4
Average %
94
Range %
89-100
1:8
Average %
102
Range %
97-106
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回收率:
The recovery of mouse WNT7A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
91
85-98
EDTA plasma (n=4)
96
90-102
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標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml
OD1
OD2
Average
Corrected
10
3.163
3.014
3.088
2.979
5
2.308
2.267
2.288
2.178
2.5
1.554
1.528
1.541
1.431
1.25
0.928
0.919
0.923
0.814
0.625
0.613
0.629
0.621
0.511
0.312
0.403
0.393
0.398
0.288
0.156
0.287
0.284
0.285
0.176
0
0.111
0.108
0.110
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數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- Involvement of Wnt7a in the role of M2c microglia in neural stem cell oligodendrogenesis M Mecha,Journal of Neuroinflammation,2020
- M2 macrophages promote myofibroblast differentiation of LR-MSCs and are associated with pulmonary fibrogenesis Hou J, et al,Cell communication and signaling,2018
- Does murine spermatogenesis require WNT signalling? A lesson from Gpr177 conditional knockout mouse models. Chen SR.et al,Cell Death Dis.,2016
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能:Ligand for members of the frizzled family of seven transmembrane receptors that functions in the canonical Wnt/beta-catenin signaling pathway. Plays an important role in embryonic development, including dorsal versus ventral patterning during limb development, skeleton development and urogenital tract development. Required for central nervous system (CNS) angiogenesis and blood-brain barrier regulation. Required for normal, sexually dimorphic development of the Mullerian ducts, and for normal fertility in both sexes. Required for normal neural stem cell proliferation in the hippocampus dentate gyrus. Required for normal progress through the cell cycle in neural progenitor cells, for self-renewal of neural stem cells, and for normal neuronal differentiation and maturation. Promotes formation of synapses via its interaction with FZD5.
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基因功能參考文獻(xiàn):
- Wnt7a induces a unique phenotype of monocyte-derived macrophages with lower phagocytic capacity and differential expression of pro- and anti-inflammatory cytokines PMID: 28872671
- experiments indicate that Reck and Gpr124 are part of the cell surface protein complex that transduces Wnt7a- and Wnt7b-specific signals in mammalian CNS epithelial cells to promote angiogenesis and regulate the BBB PMID: 28803732
- WNT7a is sufficient to restore post-pubertal endometrial gland formation. PMID: 27965056
- we found that post-ejaculated semen from fertile wild-type males was solidified and the sperm were entrapped in Wnt7aCre/+;Esr1f/f uteri, compared to the watery semen (liquefied) found in Esr1f/f controls.Kallikrein-related peptidases 3 (KLK3) and other kallikrein-related peptidases from male prostate glands are responsible for semen liquefaction by cleaving gel-forming proteins PMID: 28414719
- Data show that the WIF domain of Wnt Inhibitory Factor 1 (WIF1) is bound by C-terminal domains of Wnt proteins Wnt5a and Wnt7a at two sites. PMID: 26342861
- Wnt7a is a novel regulator of ventral midbrain neurogenesis and dopaminergic neuron axon growth and guidance. PMID: 24803261
- Truncated Wnt7a lacking the conserved palmitoylation sites retains full biological activity in skeletal muscle. PMID: 24287629
- epithelial Wnt7a and Wnt7b are possible ligands of Fzd-mediated beta-catenin (Ctnnb1)-dependent (canonical) Wnt signaling in the adjacent undifferentiated periotic mesenchyme of fibrocytes in the murine inner ear PMID: 24727668
- Binding of Wnt7a to Fzd7 leads to an activation of noncanonical Wnt signaling, resulting in directed myogenic stem cell migration and enhanced engraftment. PMID: 24711502
- Wnt7a triggers melanocyte stem cell differentiation through beta-catenin activation, and Kitl might induce following migration of melanoblasts to epidermis PMID: 23702581
- Wnt7a exercised critical control over multiple steps of neurogenesis by regulating genes involved in both cell cycle control and neuronal differentiation. PMID: 23629626
- FN and Wnt7a together regulate the homeostatic levels of satellite stem cells and satellite myogenic cells during regenerative myogenesis. PMID: 23290138
- Hyperactivated c-Met led to increased NF-kappaB signaling, which in turn, drove de novo expression of Wnt7a and overexpression of Wnt7b in Pkd1(-/-) mouse kidneys. PMID: 22677559
- Our data demonstrate that Wnt7a is the earliest known gamma-motor neuron marker PMID: 22723712
- These results indicate that WNT7A plays a critical role in postnatal uterine gland morphogenesis and function, which are important for blastocyst implantation and fertility in the adult uterus. PMID: 21508348
- Data show that female Wnt7a-Cre(+)PR(f/-) mice are infertile due to defects in embryo attachment, stromal cell decidualization, and the inability to cease estrogen-induced epithelial cell proliferation. PMID: 22155565
- Data show that the Fzd7 receptor complex was associated with Galpha(s) and PI(3)K and these components were required for Wnt7a to activate the Akt/mTOR growth pathway in myotubes. PMID: 22179044
- Wnt-7a and sonic hedgehog (Shh) interfere differently with the process of neuronal differentiation by promoting distinct stages of neuronal differentiation in neonatal neural stem cells. PMID: 20868729
- Data show that removal of canonical Wnt inhibitor DKK1 from DKK1-involuted mice results in full recovery, suggesting that canonical Wnt signaling is required for the differentiation or proliferation of TEC populations. PMID: 20161711
- Wnt7a coordinates a variety of cell and developmental pathways that guide postnatal uterine growth and hormonal responses and that disruption of these pathways leads to aberrant cell death. PMID: 15070830
- stage-specific physiological roles of Wnt signaling during neural development. PMID: 15142975
- Wnt7a acts through low density lipoprotein receptor-related protein 6 and the canonical Wnt signaling pathway during dorsal and posterior limb development in the mouse PMID: 15880584
- Thus, our findings suggest that Wnt7a overexpression disrupts normal Wnt signaling in the neural tube, resulting in defective adherens junctions and neurulation. PMID: 16154760
- We show that loss of function of Dishevelled-1 (Dvl1) mimics and enhances the Wnt7a phenotype in the cerebellum.Wnt7a/Dvl1 double mutant mice reveal a defect in neurotransmitter release at mossy fiber-granule cell synapses PMID: 16818724
- Results suggest a critical role for Wnt7a in control of neuronal progenitor maturation. PMID: 17174942
- Wnt7a induces dissociation of the adenomatous polyposis coli protein from the beta-catenin cytoplasmic complex and the interaction of APC with alpha 7-nicotinic acetylcholine receptors in hippocampal neurons. PMID: 17507554
- CNS uses the canonical Wnt signaling pathway to promote formation and CNS-specific differentiation of the organ's vasculature PMID: 19023080
- Heterozygous and homozygous Vangl2(Lp) mutant postnatal tissues exhibit similar phenotypes and polarity defects and display a 50% reduction in Wnt7a levels PMID: 19363157
- Wnt7a signaling through the planar cell polarity pathway controls the homeostatic level of satellite stem cells and hence regulates the regenerative potential of muscle PMID: 19497282
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相關(guān)疾?。?/div>Defects in Wnt7a cause the postaxial hemimelia (px) phenotype that is characterized by limb patterning defects accompanied by Mullerian duct-associated sterility in both sexes.亞細(xì)胞定位:Secreted, extracellular space, extracellular matrix. Secreted.蛋白家族:Wnt family數(shù)據(jù)庫(kù)鏈接:
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