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  4. Mouse Protein-arginine deiminase type-4(PADI4) ELISA kit

Mouse Protein-arginine deiminase type-4(PADI4) ELISA kit

  • 中文名稱：
    小鼠精氨酸脫亞胺酶4(PADI4)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL017379MO
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠精氨酸脫亞胺酶4(PADI4)酶聯(lián)免疫試劑盒（CSB-EL017379MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的PADI4含量。PADI4 是一種頗受關(guān)注的靶點(diǎn)。它在多種生理和病理過程中發(fā)揮作用。研究發(fā)現(xiàn)其參與蛋白質(zhì)瓜氨酸化過程，與自身免疫性疾病、腫瘤的發(fā)生發(fā)展等機(jī)制相關(guān)。對 PADI4 的深入研究有助于揭示疾病發(fā)病機(jī)理，為相關(guān)疾病治療提供新方向。試劑盒檢測范圍為4.7 pg/mL-300 pg/mL，適用于體外實(shí)驗(yàn)，能夠支持多種研究場景，如探索炎癥性疾病模型中PADI4的表達(dá)動態(tài)、評估基因編輯或藥物干預(yù)后酶活性變化，或分析腫瘤微環(huán)境中蛋白質(zhì)翻譯后修飾機(jī)制。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Padi4 ELISA Kit; Pad4 ELISA Kit; Pdi4 ELISA Kit; Protein-arginine deiminase type-4 ELISA Kit; EC 3.5.3.15 ELISA Kit; Peptidylarginine deiminase IV ELISA Kit; Protein-arginine deiminase type IV ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    4.7 pg/mL-300 pg/mL
  • 靈敏度：
    1.17 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Epigenetics and Nuclear Signaling
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse PADI4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %91
    Range %85-99
    1:2Average %95
    Range %89-101
    1:4Average %96
    Range %92-110
    1:8Average %93
    Range %86-102
  • 回收率：
    The recovery of mouse PADI4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9788-105
    EDTA plasma (n=4)9689-104
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    3002.228 2.230 2.229 2.051
    1501.457 1.378 1.418 1.240
    750.854 0.822 0.838 0.660
    37.50.483 0.513 0.498 0.320
    18.750.355 0.363 0.359 0.181
    9.40.274 0.259 0.267 0.089
    4.70.233 0.226 0.230 0.052
    00.175 0.181 0.178
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    Catalyzes the citrullination/deimination of arginine residues of proteins such as histones, thereby playing a key role in histone code and regulation of stem cell maintenance. Citrullinates histone H1 at 'Arg-54' (to form H1R54ci), histone H3 at 'Arg-2', 'Arg-8', 'Arg-17' and/or 'Arg-26' (to form H3R2ci, H3R8ci, H3R17ci, H3R26ci, respectively) and histone H4 at 'Arg-3' (to form H4R3ci). Acts as a key regulator of stem cell maintenance by mediating citrullination of histone H1: citrullination of 'Arg-54' of histone H1 (H1R54ci) results in H1 displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance. Promotes profound chromatin decondensation during the innate immune response to infection in neutrophils by mediating formation of H1R54ci. Required for the formation of neutrophil extracellular traps (NETs); NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation. Citrullination of histone H3 prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1.
  • 基因功能參考文獻(xiàn)：
    1. PAD4 in neutrophils is essential for kidney NET formation and ischemic kidney injury. PMID: 29061334
    2. PADi4 is critical for neutrophil extracellular traps formation, but is dispensable for generalized citrullination PMID: 28188029
    3. sFlt-1 overexpression in Padi4(-/-) mice resulted in dramatically lower inflammatory and thrombotic response, which was accompanied by significant reduction in pregnancy losses. Inhibition of NETosis may serve as a novel target in disorders of impaired placentation. PMID: 28007693
    4. a role for NETs in cardiac fibrosis and conclude that PAD4 regulates age-related organ fibrosis and dysfunction. PMID: 28031479
    5. PADI4 exacerbates arthritis with diverse immunological modifications. PMID: 26293116
    6. Selective inhibition of peptidylarginine deiminase IV with Cl-amidine blocked NET formation, reduced atherosclerotic lesion area, and delayed time to carotid artery thrombosis in a photochemical injury model. PMID: 24425713
    7. Padi4 is part of the pluripotency transcriptional network, binding to regulatory elements of key stem-cell genes and activating their expression PMID: 24463520
    8. PAD4-mediated chromatin decondensation in the neutrophil is crucial for pathological venous thrombosis and present neutrophil activation and PAD4 as potential drug targets for deep vein thrombosis. PMID: 23650392
    9. PAD4 is dispensable in this effector phase model of arthritis PMID: 22551352
    10. analysis of regulation of histone modification and chromatin structure by the p53-PADI4 pathway PMID: 22334079

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  • 亞細(xì)胞定位：
    Cytoplasm. Nucleus. Cytoplasmic granule.
  • 蛋白家族：
    Protein arginine deiminase family
  • 組織特異性：
    Expressed in pluripotent embryonic stem and induced pluripotent stem cells but not multipotent neural stem cells.
  • 數(shù)據(jù)庫鏈接：