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中文名稱:小鼠delta蛋白同源物1(DLK1)酶聯免疫試劑盒
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貨號:CSB-EL006945MO
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規(guī)格:96T/48T
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價格:¥3600/¥2500
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其他:
產品詳情
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產品描述:小鼠delta蛋白同源物1(DLK1)酶聯免疫試劑盒(CSB-EL006945MO)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的DLK1含量。DLK1是一個重要靶點。它在胚胎發(fā)育中發(fā)揮作用,且與多種疾病發(fā)生發(fā)展相關。研究機制方面,主要聚焦于其信號通路以及與其他分子的相互作用,了解其如何調控細胞增殖、分化等過程,為疾病治療靶點開發(fā)提供依據。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL,適用于小鼠模型中DLK1蛋白表達的動態(tài)監(jiān)測,可廣泛用于脂肪代謝研究、發(fā)育生物學機制探索、腫瘤微環(huán)境分析等科研領域,為探究DLK1在生理及病理過程中的調控機制提供可靠工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
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別名:Dlk1 ELISA kit; Dlk ELISA kit; Pref1 ELISA kit; Scp-1Protein delta homolog 1 ELISA kit; DLK-1 ELISA kit; Adipocyte differentiation inhibitor protein ELISA kit; Preadipocyte factor 1 ELISA kit; Pref-1) [Cleaved into: Fetal antigen 1 ELISA kit; FA1)] ELISA kit
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縮寫:
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Uniprot No.:
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種屬:Mus musculus (Mouse)
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樣本類型:serum, plasma, tissue homogenates
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檢測范圍:15.6 pg/mL-1000 pg/mL
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靈敏度:3.9 pg/mL
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反應時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領域:Neuroscience
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse DLK1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 88 Range % 84-96 1:2 Average % 95 Range % 91-99 1:4 Average % 94 Range % 90-98 1:8 Average % 102 Range % 92-108 -
回收率:
The recovery of mouse DLK1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 96 92-102 EDTA plasma (n=4) 89 82-98 -
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 1000 2.045 1.944 1.995 1.844 500 1.644 1.523 1.584 1.433 250 1.073 1.052 1.063 0.912 125 0.673 0.662 0.668 0.517 62.5 0.371 0.382 0.377 0.226 31.2 0.284 0.273 0.279 0.128 15.6 0.235 0.231 0.233 0.082 0 0.148 0.153 0.151 -
數據處理:
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貨期:3-5 working days
引用文獻
- Preadipocyte factor 1 regulates adipose tissue browning via TNF-α-converting enzyme-mediated cleavage Rhee M, et al,Metabolism,2019
- IMP2/p62 induces genomic instability and an aggressive hepatocellular carcinoma phenotype Kessler SM. et al,Cell Death Dis,2015
靶點詳情
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功能:May have a role in neuroendocrine differentiation. Inhibits adipocyte differentiation.
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基因功能參考文獻:
- Functional analysis revealed that ground-state miRNAs embedded in the Dlk1-Dio3 locus (miR-541-5p, miR-410-3p, and miR-381-3p) promoted pluripotency via inhibition of multi-lineage differentiation and stimulation of self-renewal PMID: 29129685
- This study suggests that Pref-1 is an endocrine factor which is synergistically increased by obesity and age. PMID: 29138005
- Gene body methylation of noncoding RNA genes was observed and among these microRNA genes were prominent. Of particular note, observed only in hyperphenylalaninemic animals, was hypomethylation of miRNA genes within the imprinted Dlk1-Dio3 locus on chromosome 12. PMID: 26822703
- The role of elevated oxygen levels in eroding imprinted Dlk1 expression during prolonged culture and in vitro differentiation. PMID: 27729406
- Loss of DLK1 expression is associated with fetal growth retardation complications of pregnancy. PMID: 27776119
- Pref-1 mRNA is a novel substrate of RNase-L. PMID: 27831565
- the conserved sequences in IG-DMR are involved in the expression regulation of some of the imprinted genes in the Dlk1-Dio3 domain PMID: 27904015
- To investigate the nature of these more variably methylated secondary differentially methylated regions, we adopted a hairpin linker bisulfite mutagenesis approach to examine CpG dyad methylation at differentially methylated regions associated with the murine Dlk1/Gtl2 imprinting cluster on both complementary strands. PMID: 28649282
- NOTCH1 ligand Delta-like 1 homolog (DLK1) self interacts PMID: 28099888
- the salivary gland phenotype of Dlk1 knock-out mice, was investigated. PMID: 26711912
- The brain-specific miR-379/miR-410 gene cluster at the imprinted Dlk1-Dio3 domain is implicated in several aspects of brain development and function. PMID: 26744330
- data provide the first evidence for a direct interaction between DLK1 and NOTCH1 in mammals, and substantiate that non-canonical NOTCH ligands exist, adding to the complexity of NOTCH signaling. PMID: 26791579
- the Dlk1-Gtl2 locus plays a critical role in preserving long-term repopulating HSCs (LT-HSCs). PMID: 26627594
- DNA methylation regulates genomic imprinted DLK1-Dio3 miRNAs in autoimmune lupus PMID: 27070142
- identify the molecular regulators involved in the recruitment of AFF3 to gDMRs and provide mechanistic insight into the requirement of AFF3 at an enhancer for the expression of an approximately 200-kb polycistronic transcript within the imprinted Dlk1-Dio3 locus PMID: 26728555
- Dppa3 has a critical role in generation of fully reprogrammed iPS cells and maintenance of Dlk1-Dio3 imprinting PMID: 25613421
- results suggest that maternal expression of the imprinted miR-379/miR-544 cluster regulates paternal expression of the Dlk1 gene in mice PMID: 26487685
- Dll1 and Jag1, Notch ligands, function redundantly and are necessary to maintain the centroacinar cells as an environmental niche in the developing pancreas. PMID: 25919081
- Gtl2((-/+)) teratomas have decreased expression of 28 miRNAs encoded by the Dlk1-Dio3 domain PMID: 25355544
- DLK1 is a novel inflammatory inhibitor which interferes with NOTCH1 signaling in TLR-activated murine macrophages. PMID: 26115479
- these data suggest that miR-143 promotes adipogenesis by directly modulating the pref-1 expression in adipocytes. PMID: 25366176
- This study provides the spatiotemporal expression and dynamic changes of lncRNAs from Dlk1-Dio3 imprinted region in mouse preimplantation stage embryos and offers insight into the potential mechanisms responsible for Gtl2 activation. PMID: 26005002
- Pref-1 expressing cells are required for adipose tissue development. PMID: 25088414
- Data indicate that systemic delta-like 1 protein Dll1/delta-like4 protein Dll4 inhibition decreased T cell cytokines and graft infiltration. PMID: 25687759
- the function of DLK1 is to shift the metabolic mode of the organism toward peripheral lipid oxidation and away from lipid storage PMID: 25349437
- DNA methylation dynamics of a maternally methylated DMR in the mouse Dlk1-Dio3 domain PMID: 25447521
- Multiple Dlk1 splice variants are expressed during early mouse embryogenesis. PMID: 24860997
- DLK1 regulates submandibular salivary glands morphogenesis and parasympathetic nerve fibre outgrowth through inhibition of NOTCH signalling PMID: 24828459
- Upregulation of Prdm14 results in a highly homogeneous population of authentic pluripotent colonies and prevents the abnormal silencing of the Dlk1-Dio3 locus. PMID: 24552707
- Study characterized in detail AK044800, a transcript from the Dlk1-Dio3 imprinted region. PMID: 23515577
- Dlk1 isoforms have distinctive effects on myogenesis, and its regulation during myogenesis is critical for normal muscle development. PMID: 24582655
- Dlk1 is both necessary and sufficient for determining fast motor neurons and their corresponding biophysical signature in the mouse and chick. PMID: 24626931
- Dlk1 is a negative regulator of emerging hematopoietic stem and progenitor cells. PMID: 22801971
- delta-like 1 homolog (Dlk1), an imprinted gene best known for its ability to inhibit adipogenesis, is a crucial regulator of the myogenic program in skeletal muscle. PMID: 23946446
- data suggest that loss of Dlk1 gives rise to minor pituitary defects manifesting as an age- and sex-dependent reduction in pituitary hormone contents PMID: 23279263
- data identify Dlk1-Dio3 ncRNAs as novel candidate early biomarkers for mouse liver tumor promotion and provide new opportunities for assessing the carcinogenic potential of novel compounds PMID: 23091169
- Dlk1 expression is not essential for the normal development of beta-cells, somatotrophs and endothelial cells. PMID: 23059197
- Data indicate a set of 29 up-regulated miRNAs originating from the Dlk1-Dio3 genomic imprinted region, which has been identified as a hallmark of pluripotency and proliferation. PMID: 23554452
- Data indicate that DLK+ fetal hepatic progenitors cells support long-term expansion of hematopoietic stem cells (HSCs). PMID: 23415675
- Data also found that Dlk interrupts the binding between Cfr and Fgf18. The Fgf18 signaling pathway seems to be finely tuned by Cfr and Dlk for skeletal development. PMID: 20023171
- Data found that upon liver injury, Dlk1 was dramatically induced and initially expressed in hepatocytes and then into the HSCs by a paracrine manner. PMID: 21239501
- Data suggest that only membrane-bound Dlk1 (Dlk1-M) represses preadipocyte proliferation, not soluble form (Dlk1-S). Adult Dlk1-null mice exhibit higher amounts of fat than wildtype control mice, possibly due to increased preadipocyte replication. PMID: 22891218
- localize the majority of Dlk1 cis-regulatory elements to a 41 kb region upstream of the gene PMID: 22606264
- DLK1 may have a dual role in the hypothalamus: it may play a role in the post-natal hypothalamic maturation of the arginine vasopressin and oxytocin neurons. PMID: 22563444
- the imprinted delta-like homolog 1/preadipocyte factor (Dlk1/Pref1) and iodothyronine deiodinase type 3 (Dio3) functions converge on the development of brown fat at the transition to independent life. PMID: 22326222
- crosstalk between TGF-beta1 and Dlk1 was shown to promote early chondrogenesis during the embryonic endochondral ossification process. PMID: 22102178
- Dlk1-null mice showed increased levels of NOTCH downstream targets, such as Snail and Slug PMID: 22068159
- novel roles of HSC-derived DLK1 in activating HSCs via epigenetic Ppargamma repression and participating in liver regeneration and development in a manner involving the mesenchymal-epithelial interaction. PMID: 22298767
- These findings provide evidence supporting several regulatory functions of delta-like protein 1 (DLK1) in the pituitary gland. PMID: 21756269
- Dlk1/FA1 acts as an inhibitor of the PI3K/Akt pathways that leads to its inhibitory effects on chondrogenesis. PMID: 21724852
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亞細胞定位:Membrane; Single-pass type I membrane protein. Cytoplasm.
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組織特異性:Highly expressed in fetal liver, placenta, adult adrenal gland, brain, testis and ovary and, to a lesser degree, in adult kidney, muscle, thymus and heart.
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數據庫鏈接:
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