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  4. Mouse Pulmonary Surfactant-associated protein C (SP-C) ELISA kit

Mouse Pulmonary Surfactant-associated protein C (SP-C) ELISA kit

  • 中文名稱：
    小鼠肺表面活性物質(zhì)相關(guān)蛋白C(SP-C)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E12639m
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3200/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠肺表面活性物質(zhì)相關(guān)蛋白C(SP-C)酶聯(lián)免疫試劑盒（CSB-E12639m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的SFTPC含量。SFTPC編碼表面活性蛋白C，是肺表面活性物質(zhì)的重要成分，對維持肺泡穩(wěn)定性至關(guān)重要。其突變會(huì)影響蛋白結(jié)構(gòu)與功能，干擾肺表面活性物質(zhì)代謝。相關(guān)研究聚焦突變致病機(jī)制，探索其在間質(zhì)性肺疾病發(fā)病中的作用及潛在治療靶點(diǎn)。試劑盒檢測范圍為2.3 ng/mL-150 ng/mL，適用于基礎(chǔ)科研中探究肺部疾病模型（如肺纖維化、急性肺損傷）、肺泡上皮細(xì)胞功能研究或藥物干預(yù)下SP - C的表達(dá)動(dòng)態(tài)變化。其寬泛的樣本兼容性支持體外細(xì)胞實(shí)驗(yàn)（培養(yǎng)上清）、動(dòng)物模型（血清/血漿）及組織樣本（肺勻漿）的多維度分析，為研究肺部生理病理機(jī)制提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Sftpc ELISA Kit; Sftp2 ELISA Kit; Pulmonary surfactant-associated protein C ELISA Kit; SP-C ELISA Kit; Pulmonary surfactant-associated proteolipid SPL(Val) ELISA Kit; SP5 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    2.3 ng/mL-150 ng/mL
  • 靈敏度：
    0.58 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse SP-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %86
    Range %80-92
    1:2Average %91
    Range %85-96
    1:4Average %95
    Range %90-100
    1:8Average %98
    Range %92-102
  • 回收率：
    The recovery of mouse SP-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9488-98
    EDTA plasma (n=4)9690-100
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    1502.147 2.052 2.100 2.009
    751.693 1.576 1.635 1.544
    37.51.121 1.044 1.083 0.992
    18.80.648 0.639 0.644 0.553
    9.40.429 0.424 0.427 0.336
    4.70.295 0.279 0.287 0.196
    2.30.194 0.179 0.187 0.096
    00.092 0.090 0.091 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    Pulmonary surfactant associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces.
  • 亞細(xì)胞定位：
    Secreted, extracellular space, surface film.
  • 數(shù)據(jù)庫鏈接：