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  4. Mouse Pulmonary surfactant-associated protein A,SP-A ELISA Kit

Mouse Pulmonary surfactant-associated protein A,SP-A ELISA Kit

  • 中文名稱：
    小鼠肺表面活性物質(zhì)相關(guān)蛋白A(SP-A)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E08685m
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3200/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠肺表面活性物質(zhì)相關(guān)蛋白A(SP-A)酶聯(lián)免疫試劑盒（CSB-E08685m）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的SFTPA1含量。SFTPA1是一個重要靶點(diǎn)。其它在相關(guān)生理或病理過程中可能發(fā)揮關(guān)鍵作用。研究機(jī)制上，目前可能聚焦于它在細(xì)胞信號傳導(dǎo)、生物合成等方面的功能，探索其與疾病發(fā)生發(fā)展的聯(lián)系，以尋找基于該靶點(diǎn)的治療策略。試劑盒檢測范圍為3.12 pg/mL-200 pg/mL，尤其適用于動物模型研究中的肺功能評估、呼吸道疾病機(jī)制探索以及藥物干預(yù)效果評價等科研場景，為研究者提供穩(wěn)定可靠的檢測工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Sftpa1 ELISA Kit; Sftp-1 ELISA Kit; Sftp1 ELISA Kit; Sftpa ELISA Kit; Pulmonary surfactant-associated protein A ELISA Kit; PSAP ELISA Kit; PSP-A ELISA Kit; SP-A ELISA Kit
  • 縮寫：
    SFTPA1
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    3.12 pg/mL-200 pg/mL
  • 靈敏度：
    0.78 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse SP-A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1000Average %84
    Range %80-92
    1:2000Average %96
    Range %91-105
    1:4000Average %98
    Range %93-103
    1:8000Average %93
    Range %86-98
  • 回收率：
    The recovery of mouse SP-A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9087-95
    EDTA plasma (n=4)9390-95
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    2002.637 2.714 2.676 2.549
    1002.331 2.368 2.350 2.223
    501.847 1.901 1.874 1.747
    251.273 1.267 1.270 1.143
    12.50.845 0.869 0.857 0.730
    6.250.542 0.583 0.563 0.436
    3.120.322 0.317 0.320 0.193
    00.128 0.126 0.127 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評價

靶點(diǎn)詳情

  • 功能：
    In presence of calcium ions, it binds to surfactant phospholipids and contributes to lower the surface tension at the air-liquid interface in the alveoli of the mammalian lung and is essential for normal respiration. Enhances the expression of MYO18A/SP-R210 on alveolar macrophages.
  • 亞細(xì)胞定位：
    Secreted, extracellular space, extracellular matrix. Secreted, extracellular space, surface film.
  • 蛋白家族：
    SFTPA family
  • 數(shù)據(jù)庫鏈接：