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  4. Mouse R-spondin-1(RSPO1) ELISA kit

Mouse R-spondin-1(RSPO1) ELISA kit

  • 中文名稱：
    小鼠R脊椎蛋白1(RSPO1)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL020550MO
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠R脊椎蛋白1(RSPO1)酶聯(lián)免疫試劑盒（CSB-EL020550MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的RSPO1含量。試劑盒檢測范圍為6.25 pg/mL-400 pg/mL，適用于體外研究RSPO1在發(fā)育生物學(xué)、干細(xì)胞分化、腫瘤微環(huán)境或再生醫(yī)學(xué)中的表達(dá)變化本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Rspo1R-spondin-1 ELISA kit; Cysteine-rich and single thrombospondin domain-containing protein 3 ELISA kit; Cristin-3 ELISA kit; mCristin-3 ELISA kit; Roof plate-specific spondin-1 ELISA kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    6.25 pg/mL-400 pg/mL
  • 靈敏度：
    1.56 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Developmental Biology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse RSPO1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %93
    Range %86-97
    1:2Average %101
    Range %96-105
    1:4Average %93
    Range %85-97
    1:8Average %92
    Range %84-96
  • 回收率：
    The recovery of mouse RSPO1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9081-94
    EDTA plasma (n=4)107101-111
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    4002.198 2.113 2.156 1.986
    2001.312 1.276 1.294 1.124
    1000.886 0.844 0.865 0.695
    500.504 0.531 0.518 0.348
    250.402 0.424 0.413 0.243
    12.50.319 0.333 0.326 0.156
    6.250.237 0.231 0.234 0.064
    00.171 0.169 0.170
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Has a essential roles in ovary determination. Regulates Wnt signaling by antagonizing DKK1/KREM1-mediated internalization of LRP6 through an interaction with KREM1.
  • 基因功能參考文獻(xiàn)：
    1. reciprocal control of antagonistic Wnt signaling pathways by Rspo1 in muscle stem cell progeny is a key step ensuring normal tissue architecture restoration following acute damage. PMID: 28273449
    2. In Rspo1 knock-out mice during pregnancy, a stronger expression of mesenchymal markers was observed, without modifications to the structure of mammary epithelial tissue. Mammary epithelial cell immunohistochemical analysis revealed a persistence of virgin markers, which signify a delay in cell differentiation. PMID: 27611670
    3. We also show that R-spondin1 is depleted in RANK-null progenitors, and that its exogenous administration rescues key aspects of RANK deficiency by reinstating a WNT response and mammary cell expansion PMID: 26095608
    4. R-spondin 1/dickkopf-1/beta-catenin machinery is involved in testicular embryonic angiogenesis PMID: 25910078
    5. The ascl2 is regulated in a direct autoactivatory loop, leading to a distinct on/off expression pattern, and Wnt/R-spondin can activate this regulatory loop. PMID: 25620640
    6. RSPO1, WNT4, and beta-catenin have roles in the signaling pathway during ovarian differentiation in mice. PMID: 25187620
    7. Rspo1 is required for ovarian differentiation by activation of the WNT/CTNNB1 signalling pathway PMID: 18250098
    8. Rspo1 is involved in maintenance of the immature state of the progenitors of the granulosa cells of the ovary PMID: 24036309
    9. Rspo1 is involved in the progenitor cells proliferation of the coelomic epithelium of the undifferentiated gonad of the mouse embryo PMID: 23095882
    10. ZNRF3 binds RSPO1 and LGR5-RSPO1 with micromolar affinity via RSPO1 furin-like 1 (Fu1) domain. PMID: 24349440
    11. overexpressed in culture-activated hepatic stellate cells PMID: 25218283
    12. R-spondin1 (Rspo1) as a novel hormonal mediator in the mammary gland PMID: 25260709
    13. Findings indicate that R-spondin1 (Rspo1) is a negative regulator of beta-cell neogenesis and suggest that Rspo1 may serve as a therapeutic target for the preservation or restoration of functional [beta]-cells in diabetes. PMID: 24263108
    14. R-Spondin 1 is upregulated by mechanical signals and secreted. R-Spondin 1 promotes bone formation in three mouse models of osteoporosis. PMID: 23974989
    15. Wnt/Rspondin/beta-catenin signals control axonal sorting and lineage progression in Schwann cell development. PMID: 24151333
    16. In conclusion, present study highlights the role of Rspo 1 in bone remodeling where it activates Wnt signaling to induce differentiation, as shown in human as well murine in vitro osteoblast cell models. PMID: 23617070
    17. Rspo1 is an essential activator of ovarian development not only in normal situations, but also in sex reversal situations PMID: 23300469
    18. this study identifies Rspo1 and Wnt4 as two new regulators of cell proliferation in the early gonad regardless of its sex, in addition to the specific role of these genes in ovarian differentiation. PMID: 23095882
    19. Foxl2 expression partly depends of Rspo1 signalisation. PMID: 22116255
    20. RSPO1/beta-catenin signaling is involved in meiosis in fetal germ cells and contributes to the cellular decision of germ cells to differentiate into oocyte or sperm. PMID: 21991325
    21. The study reveals a role for RSPO1 as a regulator of both beta cell proliferation and neogenesis in vivo, and reinforces the importance of cWNT signalling for the maintenance of normal pancreatic beta cell behaviour. PMID: 21484214
    22. The Wnt agonist R-spondin1 regulates systemic graft-versus-host disease by protecting intestinal stem cells. PMID: 21282378
    23. R-spondin-1 is a novel beta-cell growth factor and insulin secretagogue PMID: 20442404
    24. Intestinal ischemia-reperfusion injury may inhibit expression of RSpo1 in the early stage, and enhance its expression in the middle stage. PMID: 19726353
    25. R-spondin1, an intestinal stem cell growth factor, protects against radiation-induced gastrointestinal syndrome in mice PMID: 19956666
    26. R-spondin might be a novel marker of the boundary between the roof plate and neuroepithelium and may contribute to the development of dorsal neural tube under the regulation of Wnts. PMID: 14732490
    27. R-sponin1 (Rspo1) acted synergistically with Wnt3A to activate Wnt/beta-catenin signaling in the uncommitted mesenchymal C2C12 cells. PMID: 18242177
    28. Rspo1 is required to fully suppress the male differentiation program and to maintain germ cell survival during the development of XX gonads. PMID: 18250097
    29. Balance between Sox9 and beta-catenin activation determines the fate of the gonad, with Rspo1 acting as a crucial regulator of canonical beta-catenin signaling required for female development. PMID: 18250098
    30. RSPO1 is up-regulated in the embryonic gonads of female vertebrates with different sex-determining mechanisms. PMID: 18651984
    31. R-Spondin1 protects mice from chemotherapy or radiation-induced oral mucositis through the canonical Wnt/beta-catenin pathway PMID: 19179402
    32. SRY represses the transcriptional of the Rspo1/Wnt target genes involved in ovarian determination. PMID: 19376480
    33. these data demonstrate that local epithelial Rspo1 signalling is required for normal development of the mammary gland. PMID: 19857464

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  • 亞細(xì)胞定位：
    Secreted. Nucleus.
  • 蛋白家族：
    R-spondin family
  • 組織特異性：
    Expressed in the dorsal part of the neural tube on 10 and 12 dpc, especially in the boundary region between roof plate and neuroepithelium. This expression is enhanced in the rostral part. Also expressed in other tissues such as truncal region neighboring
  • 數(shù)據(jù)庫鏈接：