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  4. Mouse Regenerating islet-derived protein 3-gamma(REG3G) ELISA kit

Mouse Regenerating islet-derived protein 3-gamma(REG3G) ELISA kit

  • 中文名稱：
    小鼠再生胰島衍生蛋白3γ(REG3G）酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL019549MO
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠再生胰島衍生蛋白3γ(REG3G）酶聯(lián)免疫試劑盒（CSB-EL019549MO）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的REG3G含量。試劑盒檢測(cè)范圍為15.6 pg/mL-1000 pg/mL，科研人員可將其應(yīng)用于小鼠模型研究，如胰腺再生機(jī)制探索、腸道炎癥性疾病進(jìn)程分析或感染后宿主防御反應(yīng)評(píng)估，支持體外實(shí)驗(yàn)樣本中REG3G的動(dòng)態(tài)濃度檢測(cè)。為分子機(jī)制研究和生物標(biāo)志物發(fā)現(xiàn)提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Reg3g ELISA Kit; Pap3 ELISA Kit; Regenerating islet-derived protein 3-gamma ELISA Kit; REG-3-gamma ELISA Kit; Pancreatitis-associated protein 3 ELISA Kit; Regenerating islet-derived protein III-gamma ELISA Kit; Reg III-gamma) [Cleaved into: Regenerating islet-derived protein 3-gamma 16.5 kDa form; Regenerating islet-derived protein 3-gamma 15 kDa form] ELISA Kit
  • 縮寫：
    REG3G
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    15.6 pg/mL-1000 pg/mL
  • 靈敏度：
    3.9 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse REG3G in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:100Average %93
    Range %89-106
    1:200Average %92
    Range %83-100
    1:400Average %95
    Range %89-99
    1:800Average %97
    Range %89-103
  • 回收率：
    The recovery of mouse REG3G spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9491-98
    EDTA plasma (n=4)9792-105
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    10002.190 2.123 2.157 1.996
    5001.793 1.762 1.778 1.617
    2501.295 1.257 1.276 1.115
    1250.779 0.748 0.764 0.603
    62.50.562 0.554 0.558 0.397
    31.250.375 0.368 0.372 0.211
    15.60.263 0.254 0.259 0.098
    00.157 0.165 0.161
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

平均分:
5.0分 - 1 個(gè)評(píng)價(jià)

樣品類型：Serum ,細(xì)胞培養(yǎng)上清液

樣品信息：小鼠

稀釋比：其他

產(chǎn)品評(píng)價(jià)： 我使用Mouse Reg3genRating islet-derived protein 3-gamma（REG3G)ELISA kit，檢測(cè)小鼠血清內(nèi)REG3G,分別檢測(cè)了1：50，1：100，1：200的稀釋比例。以1：200為例，對(duì)照組和實(shí)驗(yàn)組的OD值分別是0.2603和0.4635，實(shí)驗(yàn)結(jié)果符合我們對(duì)實(shí)驗(yàn)前期的預(yù)測(cè)。產(chǎn)品評(píng)價(jià)：該elisa檢測(cè)試劑盒產(chǎn)品質(zhì)量過關(guān)，檢測(cè)效果好。能較好的滿足實(shí)驗(yàn)需求。

By 吳老師

靶點(diǎn)詳情

  • 功能：
    Bactericidal C-type lectin which acts exclusively against Gram-positive bacteria and mediates bacterial killing by binding to surface-exposed carbohydrate moieties of peptidoglycan. Restricts bacterial colonization of the intestinal epithelial surface and consequently limits activation of adaptive immune responses by the microbiota. The uncleaved form has bacteriostatic activity, whereas the cleaved form has bactericidal activity against L.monocytogenes and methicillin-resistant S.aureus. Regulates keratinocyte proliferation and differentiation after skin injury.
  • 基因功能參考文獻(xiàn)：
    1. the secretion of regenerating islet-derived IIIgamma (RegIIIgamma) is responsible for the protective effect of Adipose-derived stem cells. PMID: 26866937
    2. The study shows that cardiac stress activates Reg3gamma expression and p38 MAPK is an upstream regulator of Reg3gamma gene expression in heart. Altogether the data suggest Reg3gamma is associated with cardiac inflammatory signaling. PMID: 27798352
    3. regenerating islet-derived gene gamma promotes beta cell regeneration and preserves beta cells from autoimmunity damage by increasing regulatory T cell differentiation and inducing tolerated dendritic cells. PMID: 26667474
    4. Recombinant IFN-I or IFN-I expression induced by RIG-I promoted growth of intestinal organoids in vitro and production of the antimicrobial peptide regenerating islet-derived protein 3 gamma (RegIIIgamma). PMID: 28424327
    5. REG3gamma-deficient mice have altered mucus distribution and increased mucosal inflammatory responses to the microbiota and enteric pathogens in the ileum. PMID: 24345802
    6. antibacterial function for lung epithelium through Stat3-mediated induction of Reg3gamma. PMID: 23401489
    7. Reg-IIIgamma expression is mainly observed in epithelial cells of the airways and intestine, but not in the nervous system; expression levels show a gradually increasing pattern along with development. PMID: 21681751
    8. Letter: aggregation status of PAP1 and PAP2 could change their role in regulating the inflammation in acute pancreatitis. PMID: 21926556
    9. findings show that RegIIIgamma is essential for maintaining a ~50-micrometer zone that physically separates the microbiota from the small intestinal epithelial surface PMID: 21998396
    10. findings show that RegIIIgamma and its human counterpart, HIP/PAP, are directly antimicrobial proteins that bind their bacterial targets via interactions with peptidoglycan carbohydrate PMID: 16931762
    11. antibacterial activities of mouse RegIIIgamma and its human ortholog, HIP/PAP, are tightly controlled by an inhibitory N-terminal prosegment that is removed by trypsin in vivo. PMID: 19095652

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  • 亞細(xì)胞定位：
    Secreted. Cytoplasm.
  • 組織特異性：
    Predominantly expressed in the small intestine, including Paneth cells (at protein level). Hardly detectable in the colon (at protein level). Highly expressed in the lung epithelium during methicillin-resistant S.aureus infection (at protein level). Skin
  • 數(shù)據(jù)庫鏈接：