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  4. Mouse Resistin ELISA Kit
  • 中文名稱：
    小鼠抵抗素(Resistin)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E06886m
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠抵抗素(Resistin)酶聯(lián)免疫試劑盒（CSB-E06886m）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的RETN含量。RETN（抵抗素）是一種由脂肪組織分泌的激素，與胰島素抵抗和炎癥反應(yīng)密切相關(guān)。研究顯示，RETN基因多態(tài)性與多種疾病如多囊卵巢綜合征、2型糖尿病和心血管疾病等存在關(guān)聯(lián)。目前，RETN在代謝和生殖健康方面的作用成為研究熱點(diǎn)，其具體機(jī)制尚在探索中。試劑盒檢測(cè)范圍為62.5 pg/mL-4000 pg/mL，靈敏度為41.036 pg/mL。適用于探索小鼠模型中Resistin在代謝調(diào)控、炎癥通路中的作用機(jī)制，例如通過檢測(cè)肥胖模型血清Resistin水平變化，或分析脂肪組織勻漿中蛋白表達(dá)差異。其寬泛的線性范圍與多類型樣本兼容性，可支持細(xì)胞實(shí)驗(yàn)、動(dòng)物模型及離體組織研究，為糖代謝相關(guān)科研提供穩(wěn)定、可重復(fù)的數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Retn ELISA Kit; Fizz3 ELISA Kit; Resistin ELISA Kit; Adipose tissue-specific secretory factor ELISA Kit; ADSF ELISA Kit; Adipose-specific cysteine-rich secreted protein A12-alpha ELISA Kit; Cysteine-rich secreted protein FIZZ3 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測(cè)范圍：
    62.5 pg/mL-4000 pg/mL
  • 靈敏度：
    41.036 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長：
    450 nm
  • 研究領(lǐng)域：
    Cardiovascular
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse resistin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:100 Average % 96  
    Range % 91-103  
    1:200 Average % 85  
    Range % 80-90  
    1:400 Average % 93  
    Range % 89-98  
    1:800 Average % 100  
    Range % 94-105  
  • 回收率：
    The recovery of mouse resistin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 91 85-96  
    EDTA plasma (n=4) 94 90-98  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    4000 2.524 2.485 2.505 2.318  
    2000 1.917 2.007 1.962 1.775  
    1000 1.294 1.352 1.323 1.136  
    500 0.893 0.849 0.871 0.684  
    250 0.627 0.601 0.614 0.427  
    125 0.421 0.447 0.434 0.247  
    62.5 0.297 0.285 0.291 0.104  
    0 0.190 0.183 0.187    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

平均分:
5.0分 - 1 個(gè)評(píng)價(jià)

樣品類型：血清

樣品信息：小鼠

稀釋比：其他 1：200

產(chǎn)品評(píng)價(jià)： 我用 CSB-E06886m檢測(cè)高脂飲食誘導(dǎo)的C57小鼠，分別包括正常飲食、高脂誘導(dǎo)模型組及高脂誘導(dǎo)給藥組的小鼠血清，稀釋比例為1：200；實(shí)驗(yàn)流程簡(jiǎn)單易學(xué)習(xí)，易上手，且檢測(cè)結(jié)果準(zhǔn)確！

By 丁老師

靶點(diǎn)詳情

  • 最新研究進(jìn)展：
    RETN是一個(gè)脂肪細(xì)胞因子，也被稱為“Resistin”，可以影響體內(nèi)脂肪代謝和胰島素敏感性。最近的研究表明，RETN可能參與肥胖、糖尿病、心血管疾病、炎癥等疾病的發(fā)生和發(fā)展，成為一個(gè)重要的生物標(biāo)志物。
  • 功能：
    Hormone that seems to suppress insulin ability to stimulate glucose uptake into adipose cells. Potentially links obesity to diabetes.
  • 基因功能參考文獻(xiàn)：
    1. Resistin inhibited the activation of CREB, resulting in suppression of PGC-1alpha. Importantly, overexpressing PGC-1alpha can rescue the inhibitory effects of resistin on fatty acid beta oxidation. PMID: 29730652
    2. Resistin-associated VSMC dysfunction and intimal hyperplasia are related to PKCepsilon-dependent Nox activation and ROS generation PMID: 27573736
    3. OLI is a physiological repressor of systemic resistin release whereas FFA upregulate resistin release in vitro from adipocytes. PMID: 26285069
    4. Resistin possibly acts via an intracrine pathway as an intracellular sensor, regulating the adipocyte insulin sensitivity. PMID: 26036220
    5. Mouse Resistin has regulative effects on murine bone marrow hematopoiesis. PMID: 26572487
    6. murine resistin is increased in the lungs of wild-type mice following acute ozone exposure but does not promote ozone-induced lung pathology. PMID: 26386120
    7. Inhibition of miR-696 restored the triglycerides content by up to 80%, which suggests that, in C2C12 cells, resistin at least partially increases the deposition of lipids through miR-696. PMID: 25962325
    8. Antiresistin RNA oligo ameliorated metabolic abnormalities and histopathology of nonalcoholic fatty liver disease through attenuating proinflammatory cytokines PMID: 25922835
    9. Resistin regulates PAI-1 expression in 3T3-L1 adipocytes via Akt phosphorylation. PMID: 24667608
    10. Oxidized-LDL promotes the expression and secretion of visfatin and resistin through its activation of endoplasmic reticulum stress. PMID: 24278099
    11. ET type-A receptor, ERK1/2, JNKs, AKT, and JAK2, but not ET type-B receptor or p38, are necessary for the ET-1 stimulation of resistin gene expression PMID: 24424064
    12. resistin is a negative regulator of SIRT1 in both human hepatoma cell line HepG2 and mouse hepatocytes. PMID: 23827175
    13. Both resistin gene expression and its secretion from the epididymal adipose tissue were decreased in db/db obese mice, while the insulin-sensitizing drug rosiglitazone increased resistin production. PMID: 23776497
    14. lipid content was significantly decreased in 3T3-L1 cells transfected with anti-resistin small interfering RNA (siRNA) after adipocyte differentiation PMID: 24034627
    15. The effect between resistin and adiponectin on the proliferation of vascular smooth muscle cells (VSMCs), was investigated. PMID: 23267839
    16. The current study was initiated to determine whether resistin has additional roles in glucose-dependent insulinotropic polypeptide -regulated adipocyte functions. PMID: 23002036
    17. significant changes in fasting-induced adipose factor (FIAF), resistin (Rstn), long-form leptin receptor (LepRb) and suppressor of cytokine-3 (SOCS3) gene expression following chronic SCI and with advanced age PMID: 22815920
    18. results identify resistin as a potential inhibitory ligand for the receptor ROR1 and demonstrate, for the first time, that ROR1 plays an important role in adipogenesis and glucose homeostasis in 3T3-L1 cellsand glucose homeostasis in 3T3-L1 cells. PMID: 22074948
    19. Cleavage product of decorin serves as a functional receptor of resistin in adipocyte progenitors and may regulate white adipose tissue expansion. PMID: 21683670
    20. review of evidence that resistin (secreted by adipocytes in the mouse) is an inflammatory biomarker and a potential mediator of obesity, diabetes and cardiovascular disease [REVIEW] PMID: 21497511
    21. Valsartan did not alter resistin expression in the ob/ob mouse. PMID: 20472602
    22. Data suggest that s-resistin isoform may play a role in inflammatory processes. PMID: 20627112
    23. data suggest that endogenous resistin might be capable of fine-tuning the expression and enzymatic activity of various hypothalamic targets previously implicated in the delicate homeostatic control of food intake PMID: 19646421
    24. Tumor necrosis factor alpha is a negative regulator of resistin gene expression and secretion in 3T3-L1 adipocytes PMID: 11689013
    25. Mechanisms regulating adipocyte expression of resistin PMID: 11901161
    26. examined the effect on resistin expression of various hormones and cytokines known to affect insulin sensitivity. PMID: 12031960
    27. Data demonstrate that basal and hormonal stimulation of resistin secretion by insulin is inhibited by ET-1. PMID: 12163029
    28. results indicate that the substantial reduction of adiposity in lactation does not lead to any changes in resistin gene expression in white adipose tissue PMID: 12163041
    29. expression and regulation in mouse pituitary; data suggest that local resistin expression could have functional implications during prepubertal maturation of the hypothalamic-pituitary system PMID: 12208498
    30. during in vivo bone formation and remodeling, probably acting as a co-factor or accessory protein that modulates the effects of primary signaling molecules PMID: 12413946
    31. Resistin expression is regulated by C-EBP Proteins, peroxisome proliferator-activated receptor, and signal-transducing molecules. PMID: 12437985
    32. Resistin is regulated and expressed in mouse brown adipocytes PMID: 12482590
    33. resistin does not alter insulin receptor signaling but does affect insulin-stimulated glucose uptake, presumably by decreasing the intrinsic activity of cell surface glucose transporters PMID: 12618360
    34. ADSF/resistin secretion controls fat cell differentiation and adipose tissue development. PMID: 12660879
    35. Despite severe weight loss and significant falls in leptin expression and insulin concentration, resistin gene expression appears unchanged in white adipose tissue of mice with MAC16 tumour. PMID: 12660881
    36. Data show that neuropeptie Y significantly increased resistin mRNA expression in white adipose tissue compared with artificial cerebrospinal fluid treated controls. PMID: 12684710
    37. resistin is a novel secreted factor first identified as a TZD-suppressible gene PMID: 12700889
    38. results demonstrate that leptin decreases resistin expression and suggest that resistin may influence glucose homeostasis PMID: 14962997
    39. mice lacking resistin exhibit low blood glucose levels after fasting, due to reduced hepatic glucose production; data support a physiological function for resistin in the maintenance of blood glucose during fasting PMID: 14976316
    40. Inhibits insulin-stimulaed glucose uptake into skeletal muscle cells without affecting insulin signaling pathways. Review. PMID: 15055467
    41. demonstrates in vivo the inhibitory role of ADSF in adipogenesis PMID: 15090646
    42. Pituitary resistin expression is age- and gender-dependent. In ob/ob and fasted mice, resistin is regulated in a tissue-specific manner. Visceral fat obesity increases but starvation decreases resistin mRNA. PMID: 15103228
    43. Plasma resistin of leptin-treated mice was barely decreased. PMID: 15126241
    44. Chronic hyperresistinemia impairs normal glucose metabolism. PMID: 15189975
    45. Nutritional regulation of resistin and changes in resistin gene expression and circulating levels in obesity are mediated, at least in part, through insulin and glucose. PMID: 15220189
    46. results indicate that chronic "hyper-resistinemia" leads to whole-body insulin resistance involving impaired insulin signaling in skeletal muscle, liver, and adipose tissue, resulting in glucose intolerance, hyperinsulinemia, and hypertriglyceridemia PMID: 15254589
    47. These results provide strong support for a physiological role of resistin in the development of hepatic insulin resistance. PMID: 15254590
    48. Article reviews recent studies of Resistin/ADSF/FIZZ3 in transgenic and knockout mice. PMID: 15358276
    49. Resistin overexpression induces dyslipidemia in mice, which is commonly seen in the insulin-resistant state, partially through enhanced secretion of lipoproteins. PMID: 15471967
    50. gender differences in resistin, adiponectin and leptin mRNA expression and plasma levels were not ablated by castration and seem to be dependent on other factors in addition to gonadal steroids PMID: 15483213

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Resistin/FIZZ family
  • 組織特異性：
    Expressed in white but not brown adipose tissue in a variety of organs.
  • 數(shù)據(jù)庫鏈接：

    KEGG: mmu:57264

    STRING: 10090.ENSMUSP00000012849

    UniGene: Mm.1181