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  4. Mouse Retinol-binding protein,RBP ELISA Kit

Mouse Retinol-binding protein,RBP ELISA Kit

  • 中文名稱：
    小鼠視黃醇結(jié)合蛋白(RBP)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E09151m
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠視黃醇結(jié)合蛋白(RBP)酶聯(lián)免疫試劑盒（CSB-E09151m）為競爭法ELISA試劑盒，定量檢測血清、血漿、尿液、組織勻漿樣本中的RBP4含量。RBP4即視黃醇結(jié)合蛋白4，是一種在肝臟合成并分泌至血液的載體蛋白。它參與視黃醇的轉(zhuǎn)運(yùn)和代謝過程。研究發(fā)現(xiàn)其異常表達(dá)與多種疾病如糖尿病、心血管疾病等相關(guān)，機(jī)制可能包括影響胰島素敏感性、脂肪代謝等。試劑盒檢測范圍為0.312 μg/mL-20 μg/mL，為研究維生素A代謝通路、構(gòu)建相關(guān)疾病動(dòng)物模型或評估藥物干預(yù)效果提供可靠的檢測工具，可滿足基礎(chǔ)科研中對RBP動(dòng)態(tài)變化的定量監(jiān)測需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Rbp4 ELISA Kit; Retinol-binding protein 4 ELISA Kit; Plasma retinol-binding protein ELISA Kit; PRBP ELISA Kit; RBP ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, urine, tissue homogenates
  • 檢測范圍：
    0.312 μg/mL-20 μg/mL
  • 靈敏度：
    0.078 μg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse RBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 92    
    Range % 86-96    
    1:2 Average % 95    
    Range % 90-103    
    1:4 Average % 88    
    Range % 85-93    
    1:8 Average % 87    
    Range % 83-92    
  • 回收率：
    The recovery of mouse RBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 99 93-104    
    EDTA plasma (n=4) 94 90-97    
                   
                   
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    μg/ml OD1 OD2 Average      
    20 0.157 0.145 0.151      
    10 0.259 0.264 0.262      
    5 0.369 0.385 0.377      
    2.5 0.587 0.545 0.566      
    1.25 0.830 0.823 0.827      
    0.625 1.278 1.233 1.256      
    0.312 1.646 1.696 1.671      
    0 2.622 2.745 2.684      
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    Retinol-binding protein that mediates retinol transport in blood plasma. Delivers retinol from the liver stores to the peripheral tissues. Transfers the bound all-trans retinol to STRA6, that then facilitates retinol transport across the cell membrane.
  • 基因功能參考文獻(xiàn)：
    1. RBP4 is involved in all-trans retinoic acid-induced cleft palate. PMID: 28849085
    2. RBP4-induced inflammation is largely mediated by TLR4. PMID: 28400700
    3. New insights into ghrelin cell physiology, and given the known functions of RBP4 and TTR, support an emerging role for the ghrelin cell in blood glucose handling and metabolism. PMID: 23840311
    4. Retinal degeneration in RBP4-Tg mice is RBP4-dependent and light-independent. PMID: 28813718
    5. Rbp4-deficient mice accumulated retinol in the liver but it was undetectable in the serum, indicating an inverse relation between serum and liver retinol levels. RBP4 is critical for the mobilization of retinol from hepatic storage pools, and such mobilization is necessary for ocular development and visual function. PMID: 26974396
    6. Hepatocytes Are the Principal Source of Circulating RBP4 in Mice PMID: 27797907
    7. RBP4 may be a critical modulator promoting the vicious cycle of insulin resistance and heart failure by activating TLR4/MyD88-mediated inflammatory pathways. Potentially, lowering RBP4 might break the vicious cycle and improve both insulin resistance and cardiac hypertrophy. PMID: 27100622
    8. Data (including data from studies in knockout mice) suggest that Rbp4 (plasma retinol-binding protein 4) is critical for antigen presentation and activation of CD4-positive T-lymphocyte in development of insulin resistance in mice obese due to high-fat diet. PMID: 26936962
    9. A novel mechanism for circadian regulation of RBP4, but also a critical role of RBP4, acting as a hepatokine in the regulation of glucose metabolism by the circadian clock. PMID: 26564180
    10. Elevated serum RBP4 raises BP. PMID: 25911613
    11. These data show that decreasing circulating TTR levels or altering TTR-RBP4 binding could be a potential therapeutic approach for the treatment of type 2 diabetes. PMID: 25524914
    12. findings suggest that RBP4 impaired in vivo adipogenesis, partly through the repression of the insulin pathway PMID: 24590924
    13. The data implicate the involvement of chondrocytic Rbp4 in bone growth, particularly in the formation of the secondary ossification center of the limb. PMID: 23224267
    14. STRA6 in tissues other than the eye appears to be the coupling of circulating holo-RBP levels to cell signaling, in turn regulating key processes such as insulin response. PMID: 23839944
    15. expression of RBPR2 in liver and fat suggests a possible role in mediating established metabolic actions of RBP4 in those tissues PMID: 23105095
    16. TTR blocks the ability of holo-retinol-binding protein to associate with STRA6 and thereby effectively suppresses both STRA6-mediated retinol uptake and STRA6-initiated cell signaling. PMID: 22826435
    17. Suggest that RBP4 may be involved in the dyslipidemia associated with polycystic ovary syndrome in nonobese adolescents and that there may be an independent relationship between RBP4 and triglycerides but not between RBP4 and insulin resistance. PMID: 22341881
    18. RBP4 is not only an adipocytokine, but also a hepatic cytokine leading to metabolic syndrome, non-alcoholic fatty liver disease and type 2 diabetes. PMID: 21983273
    19. results indicate that rimonabant may improve vascular function by modulating RBP4 along with pro-inflammatory cytokines PMID: 21585349
    20. Basal and cyclic AMP-induced Rbp4 transcription is regulated by a multiprotein complex that is similar to ones that modulate expression of genes of steroid hormone biosynthesis. PMID: 19389484
    21. RBP4 is an adipocyte-derived 'signal' that may contribute to the pathogenesis of type 2 diabetes PMID: 16034410
    22. Lowering transthyretin(TTR) levels or interfering with RBP4-TTR binding may enhance insulin sensitivity in obesity and type 2 diabetes PMID: 18285525
    23. These results reveal a selective effect of all-trans retinoic acid inhibiting RBP4 expression specifically in adipocytes. PMID: 18769064
    24. Identification and characterization of a non-retinoid ligand for retinol-binding protein 4 which lowers serum retinol-binding protein 4 levels in vivo. PMID: 19147488
    25. HMGA1 is an important modulator of RBP4 gene expression in vivo. PMID: 19460132
    26. Although mice lacking HMGA1 are diabetic and express very low levels of the insulin receptor, they have increased insulin sensitivity. Changes in circulating Rbp4 might partly account for this paradox. [REVIEW] PMID: 19664187

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Calycin superfamily, Lipocalin family
  • 數(shù)據(jù)庫鏈接：

    KEGG: mmu:19662

    STRING: 10090.ENSMUSP00000025951

    UniGene: Mm.2605