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  4. Mouse Secreted frizzled-related protein 4(SFRP4) ELISA kit

Mouse Secreted frizzled-related protein 4(SFRP4) ELISA kit

  • 中文名稱：
    小鼠分泌型卷曲相關(guān)蛋白4(SFRP4)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL021140MO
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠分泌型卷曲相關(guān)蛋白4(SFRP4)酶聯(lián)免疫試劑盒（CSB-EL021140MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的SFRP4含量。SFRP4即分泌型卷曲相關(guān)蛋白4，它主要對Wnt信號通路進(jìn)行負(fù)調(diào)控，在胚胎發(fā)育、細(xì)胞增殖等過程發(fā)揮作用。目前研究發(fā)現(xiàn)其表達(dá)異常與多種疾病如糖尿病、腫瘤等密切相關(guān)，深入探究其機(jī)制或?yàn)橄嚓P(guān)疾病治療提供新靶點(diǎn)。試劑盒檢測范圍為0.47 ng/mL-30 ng/mL，該產(chǎn)品適用于探索SFRP4在骨骼發(fā)育、腫瘤微環(huán)境調(diào)控、糖尿病相關(guān)代謝紊亂等科研場景中的動態(tài)表達(dá)特征，為構(gòu)建疾病模型、驗(yàn)證分子機(jī)制提供可靠工具，特別適用于需要多類型樣本檢測的體外實(shí)驗(yàn)研究。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Sfrp4 ELISA Kit; Secreted frizzled-related sequence protein 4 ELISA Kit; FRP-4 ELISA Kit; sFRP-4 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    0.47 ng/mL-30 ng/mL
  • 靈敏度：
    0.11 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Stem Cells
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse SFRP4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %89
    Range %84-92
    1:2Average %100
    Range %95-105
    1:4Average %88
    Range %84-93
    1:8Average %107
    Range %104-110
  • 回收率：
    The recovery of mouse SFRP4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9390-96
    EDTA plasma (n=4)8985-92
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    302.809 2.869 2.839 2.631
    152.231 2.268 2.250 2.042
    7.51.514 1.567 1.541 1.333
    3.750.845 0.800 0.823 0.615
    1.870.585 0.575 0.580 0.372
    0.940.462 0.469 0.466 0.258
    0.470.342 0.344 0.343 0.135
    00.209 0.207 0.208
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點(diǎn)詳情

  • 功能：
    Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP4 plays a role in bone morphogenesis. May also act as a regulator of adult uterine morphology and function. May also increase apoptosis during ovulation possibly through modulation of FZ1/FZ4/WNT4 signaling. Has phosphaturic effects by specifically inhibiting sodium-dependent phosphate uptake.
  • 基因功能參考文獻(xiàn)：
    1. SFRP4 expression levels were gradually increased and proportionally associated with epididymal white adipose tissue adipocyte differentiation toward maturation at 14 days, while inguinal white adipose tissue adipocyte just showed an opposite tendency. PMID: 29730658
    2. Results indicate that sFRP4 plays a critical role in bone development and remodeling by regulating osteoblasts and osteoclasts, and that its functional loss prevents age-related bone loss in the trabecular bone. These findings imply that it functions as a potential balancer of the Wnt signaling pathway by direct influencing bone formation and absorption during skeletal bone development and maintenance through remodeling. PMID: 27117872
    3. the cross-talk between SFRP4, integrin alpha1beta1, and Notch1 suppresses the cardiac differentiation of P19CL6 cells. PMID: 27542621
    4. Our study showed that Pyle's disease was caused by a deficiency of sFRP4 and that sFRP4-mediated cross-regulation between Wnt and BMP signaling was critical for achieving proper cortical-bone thickness and stability. PMID: 27355534
    5. MicroRNA-124 regulates cell specification in the cochlea through modulation of Sfrp4 and Sfrp5. PMID: 26387953
    6. In diet-induced obesity, loss of SFRP4 alters body length and bone mineral density as well as energy expenditure and food intake. However, SFRP4 does not control glucose homeostasis and beta-cell mass in mice. PMID: 26406932
    7. MG-derived oxidative stress (not CpG demethylation) epigenetically and rapidly derepress sFRP-4 gene expression. PMID: 25036934
    8. findings suggest that an epigenetic event is critically involved in the pathogenesis of psoriasis, and the downregulation of SFRP4 by CpG island methylation is one possible mechanism contributing to the hyperplasia of epidermis in the disease PMID: 25825452
    9. extra-follicular modulators Bmp2, Dkk1, and Sfrp4 increase in early anagen. PMID: 24618599
    10. Data suggest that Sfrp4 does not contribute to the long-term regulation of serum phosphate levels in mice. PMID: 21427221
    11. sFRP4 negatively regulates bone formation without disrupting phosphorus homeostasis. PMID: 20472109
    12. the first promoter analysis of mouse Frp genes providing the basis for understanding the functions and the regulation of Frp and its role in regulating Wnt signals PMID: 12974383
    13. beta-catenin signaling is activated in tubular epithelial and interstitial cells after renal injury, and recombinant sFRP4 interferes with epithelial de-differentiation and fibroblast differentiation and function during progression of renal fibrosis PMID: 15944336
    14. Sfrp4 negatively regulates bone formation and decreases BMD through the inhibition of Wnt signaling PMID: 17002585
    15. These results indicate that Sfrp4 decreases bone formation at least in part by attenuating canonical Wnt signaling in vivo. PMID: 17907918
    16. SFRP4 may modulate Wnt functions in Tsk skin fibrosis. PMID: 17943183

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Secreted frizzled-related protein (sFRP) family
  • 組織特異性：
    Expressed in the ovary. Localized to granulosa cells of periovulatory follicles and corpora lutea. Weakly expressed in adult tissues including kidney, brain and lung.
  • 數(shù)據(jù)庫鏈接：