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  4. Mouse Semaphorin-3D(SEMA3D) ELISA kit

Mouse Semaphorin-3D(SEMA3D) ELISA kit

  • 中文名稱：
    小鼠臂板蛋白-3D(SEMA3D)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL020983MO
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠臂板蛋白-3D(SEMA3D)酶聯(lián)免疫試劑盒（CSB-EL020983MO）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的SEMA3D含量。試劑盒檢測(cè)范圍為23.5 pg/mL-1500 pg/mL，適用于研究SEMA3D在神經(jīng)系統(tǒng)疾病、腫瘤微環(huán)境調(diào)控或免疫應(yīng)答機(jī)制中的功能，尤其適合探索其在神經(jīng)軸突導(dǎo)向、細(xì)胞遷移及組織再生等生物過程中的分子機(jī)制，為神經(jīng)生物學(xué)、免疫學(xué)及腫瘤學(xué)等基礎(chǔ)研究領(lǐng)域提供高效的分析工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Sema3d ELISA Kit; Semaphorin-3D ELISA Kit
  • 縮寫：
    SEMA3D
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    23.5 pg/mL-1500 pg/mL
  • 靈敏度：
    5.8 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Neuroscience
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse SEMA3D in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %87
    Range %84-95
    1:2Average %95
    Range %91-98
    1:4Average %99
    Range %96-102
    1:8Average %96
    Range %89-103
  • 回收率：
    The recovery of mouse SEMA3D spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8680-94
    EDTA plasma (n=4)9892-104
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    15002.433 2.332 2.383 2.224
    7502.049 1.928 1.989 1.830
    3751.339 1.318 1.329 1.170
    187.50.727 0.741 0.734 0.575
    940.485 0.496 0.491 0.332
    470.280 0.291 0.286 0.127
    23.50.221 0.225 0.223 0.064
    00.159 0.158 0.159 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Induces the collapse and paralysis of neuronal growth cones. Could potentially act as repulsive cues toward specific neuronal populations. Binds to neuropilin.
  • 基因功能參考文獻(xiàn)：
    1. Authors did not find evidence for genetic interaction between Ret and Sema3d affecting survival, presence of myenteric plexus or intestine transcriptome. PMID: 28334784
    2. Data indicate that annexin A2 (AnxA2) regulates the secretion of semaphorin 3D (Sema3D) from pancreatic ductal adenocarcinoma (PDA) cells. PMID: 26243191
    3. Semaphorin 3d requires neuropilin 1 or PI3K/Akt, whereas semaphorin 3e requires plexin D1 in directing endothelial motility. PMID: 24825896
    4. In the absence of Sema3d, endothelial tubes form in a region that is normally avascular PMID: 23685842
    5. Two proepicardial markers (Scleraxis and Semaphorin3D) were identified that genetically delineate heretofore uncharacterized proepicardial subcompartments. PMID: 22421048
    6. Sema 3D and Sema 3F genes are overlappingly and distinctly expressed in the developing parathyroid and thymus, respectively, and they might play roles in the interactions between these organs and surrounding tissues PMID: 18489001

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Semaphorin family
  • 數(shù)據(jù)庫(kù)鏈接：