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  4. Mouse Soluable Thrombomodulin (STM) ELISA KIT

Mouse Soluable Thrombomodulin (STM) ELISA KIT

  • 中文名稱(chēng)：
    小鼠可溶性血栓調(diào)節(jié)蛋白 (sTM)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E13383m
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠可溶性血栓調(diào)節(jié)蛋白 (sTM)酶聯(lián)免疫試劑盒（CSB-E13383m）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的THBD含量。THBD即血栓調(diào)節(jié)蛋白，其在維持血管內(nèi)凝血與抗凝平衡、抗炎等方面有重要背景意義。研究機(jī)制上，它可與凝血酶結(jié)合，改變凝血酶性質(zhì)，激活蛋白C抗凝系統(tǒng)，抑制凝血，還能減輕炎癥反應(yīng)，在血栓性疾病等研究中受關(guān)注。試劑盒檢測(cè)范圍為0.156 ng/mL-10 ng/mL，適用于心血管疾病模型、血栓形成機(jī)制研究、內(nèi)皮功能評(píng)估等科研領(lǐng)域，尤其可用于動(dòng)脈粥樣硬化、糖尿病血管病變或敗血癥等動(dòng)物模型的生物標(biāo)志物分析。為血管生物學(xué)、凝血病理機(jī)制及藥物干預(yù)效果評(píng)估提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    Thbd; Thrombomodulin; TM; Fetomodulin; CD antigen CD141
  • 縮寫(xiě)：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類(lèi)型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    0.156 ng/mL-10 ng/mL
  • 靈敏度：
    0.039 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Cardiovascular
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse STM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:5 Average % 88
    Range % 81-92
    1:10 Average % 107
    Range % 103-110
    1:20 Average % 86
    Range % 83-89
    1:40 Average % 95
    Range % 91-99
  • 回收率：
    The recovery of mouse STM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 84 81-86
    EDTA plasma (n=4) 89 85-93
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    10 2.657 2.503 2.580 2.447
    5 1.743 1.698 1.721 1.588
    2.5 0.967 0.997 0.982 0.849
    1.25 0.572 0.591 0.582 0.449
    0.625 0.416 0.421 0.419 0.286
    0.312 0.293 0.286 0.290 0.157
    0.156 0.194 0.187 0.191 0.058
    0 0.132 0.134 0.133 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated.
  • 基因功能參考文獻(xiàn)：
    1. macrophage infiltration and neointima formation following arterial ligation-induced vascular injury were higher in wild-type than in myeloid-specific thrombomodulin-deficient mice PMID: 27808085
    2. discovery of a novel physiological function of thrombomodulin-dependent activated protein C generation within the CNS PMID: 27590316
    3. The present study found that the fifth epidermal growth factor-like domain of thrombomodulin (TME5) possesses the cytoprotective function in association with an increase in levels of anti-apoptotic myeloid cell leukemia-1 protein in an activated protein C-independent PMID: 27427915
    4. the lectin-like domain of thrombomodulin is critically involved in host defence against E. coli induced acute pyelonephritis PMID: 26573396
    5. Identified Nur77/Nor1 as novel regulators of thrombomodulin expression and function in vascular endothelial cells. PMID: 26634653
    6. TM expression in corneal epithelium was modulated during the corneal wound healing process, and may be regulated by PDGF-BB. In addition, rTMD23 has therapeutic potential in corneal injury PMID: 25816372
    7. Membrane-bound TM in macrophages plays an essential role in the development of abdominal aortic aneurysms by enhancing proinflammatory mediator elaboration, macrophage recruitment, and oxidative stress. PMID: 26338301
    8. Thrombomodulin contributes to gamma tocotrienol-mediated lethality protection and hematopoietic cell recovery in irradiated mice PMID: 25860286
    9. the recombinant epidermal growth factor-like domain plus serine/threonine-rich domain of thrombomodulin suppresses the inflammatory response in mice PMID: 25609841
    10. Thrombomodulin may modulate allergic responses by inhibiting the activation of both eosinophils and mast cells. PMID: 25497974
    11. Thrombomodulin modulates dendritic cells via both antagonism of high mobility group protein B1 and an independent mechanism. PMID: 24368584
    12. The lectin-like domain of TM does not play an important role in the host response to M. tuberculosis infection in mice. PMID: 24136651
    13. Recombinant thrombomodulin protects mice against histone-induced lethal thromboembolism. PMID: 24098750
    14. TM has a critical role in skin differentiation and wound healing. PMID: 23321922
    15. TM's lectin-like domain constrains glucose-induced complement activation on endothelial cells and podocytes and ameliorates albuminuria and glomerular damage. PMID: 23014597
    16. FFAs inhibit TM-EPCR-Protein C system in endothelial cells through activating JNK signaling, which may be a mechanism for the prothrombotic state in metabolic syndrome. PMID: 22903729
    17. Genetic or acquired thrombomodulin defects might have an impact on the severity of microangiopathic lesions after exposure to Shiga toxin-producing E. coli infections. PMID: 22942429
    18. we conclude that monocytic membrane-bound thrombomodulin participates in the LPS- and gram-negative bacteria-induced inflammatory response. PMID: 22573811
    19. Recombinant thrombomodulin domains 2 and 3 protein significantly reduces atherosclerosis and neointima formation through its thrombin-binding ability. PMID: 21840881
    20. RHBDL2 and soluble thrombomodulin were upregulated in ex vivo tissue culture of injured mouse skin. 3,4-Dichloroisocoumarin inhibited thrombomodulin production and wound healing; this was reversed by recombinant thrombomodulin in mice. PMID: 21833011
    21. Mutations in Thrombomodulin is associated with metastasis through a mechanism linked to the thrombin binding domain but not the lectin-like domain. PMID: 21788337
    22. During cardiac hypertrophy, thrombomodulin treatment decreases cardiomyocyte apoptosis and maintains left ventricular contractile function in response to pressure overload. PMID: 21111988
    23. Thrombomodulin and platelet-derived growth factor receptor alpha (PDGFRalpha) identify a population of fibroblastic reticular cells in lymph nodes where chemokine secretion is controlled by junction adhesion molecule 3 protein (JAM-C). PMID: 21685324
    24. Paclitaxel downregulates thrombomodulin expression in aortic endothelial cells. PMID: 21423464
    25. Age-dependent vulnerability to endotoxemia is associated with reduction of anticoagulant factors activated protein C and thrombomodulin. PMID: 20348393
    26. lectin-like domain of TM interferes with polymorphonuclear leukocyte adhesion to ECs by intercellular adhesion molecule 1-dependent and -independent pathways through the suppression of extracellular signal-regulated kinase activation PMID: 12208873
    27. These findings show a new function for the thrombomodulin-protein C system in controlling the growth and survival of trophoblast cells in the placenta, which is essential for the maintenance of pregnancy. PMID: 12579195
    28. Deficiency in TM results in enhanced thrombus formation in a murine model of carotid artery thrombosis and support a role for TM defects in arterial thrombotic disease PMID: 14556079
    29. thrombomodulin generates activated protein C but does not play a role in the pulmonary response to respiratory pathogens or lipopolysaccharide PMID: 14592828
    30. thrombomodulin has an antimetastatic role in reducing cell proliferation and enhancing Ca2+-independent cell aggregation PMID: 15448005
    31. surviving Thrombomodulin activated protein C generation mice displayed increased lung inflammation accompanied by higher mycobacterial loads in liver and spleen PMID: 16014564
    32. analysis of the role of TM in modulating innate immunity PMID: 16879225
    33. peripheral clock in vascular endothelial cells regulates TM gene expression and that the oscillation of TM expression may contribute to the circadian variation of cardiovascular events PMID: 17848551
    34. Par4 deficiency of the mother, or the absence of maternal platelets, restores normal development in one-third of Thbd-null embryos PMID: 18490515
    35. the lectin-like domain of thrombomodulin protects against ischaemia-reperfusion lung injury PMID: 18508817
    36. Ets-1 mediates platelet-derived growth factor-BB-induced thrombomodulin expression in human vascular smooth muscle cells. PMID: 19091791

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  • 亞細(xì)胞定位：
    Membrane; Single-pass type I membrane protein.
  • 組織特異性：
    Endothelial cells are unique in synthesizing thrombomodulin.
  • 數(shù)據(jù)庫(kù)鏈接：