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  4. Mouse Spondin-1(SPON1) ELISA kit

Mouse Spondin-1(SPON1) ELISA kit

  • 中文名稱(chēng)：
    小鼠脊椎蛋白-1(SPON1)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL022599MO
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠脊椎蛋白-1(SPON1)酶聯(lián)免疫試劑盒（CSB-EL022599MO）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的SPON1含量。小鼠脊椎蛋白-1（SPON1）是一種在脊椎動(dòng)物中廣泛表達(dá)的蛋白質(zhì)，參與細(xì)胞外基質(zhì)構(gòu)建和細(xì)胞粘附。其研究涉及細(xì)胞信號(hào)傳導(dǎo)、組織修復(fù)和腫瘤發(fā)生等機(jī)制，對(duì)理解脊椎發(fā)育和疾病治療具有重要意義。試劑盒檢測(cè)范圍為0.156 ng/mL-10 ng/mL，適用于實(shí)驗(yàn)室環(huán)境下的小鼠模型研究，如探究骨骼發(fā)育、神經(jīng)系統(tǒng)疾病或腫瘤微環(huán)境中SPON1的調(diào)控機(jī)制，亦可應(yīng)用于基因編輯動(dòng)物或藥物干預(yù)實(shí)驗(yàn)中生物標(biāo)志物的動(dòng)態(tài)監(jiān)測(cè)本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    Spon1 ELISA Kit; Spondin-1 ELISA Kit; F-spondin ELISA Kit
  • 縮寫(xiě)：
    SPON1
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類(lèi)型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    0.156 ng/mL-10 ng/mL
  • 靈敏度：
    0.039 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Neuroscience
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線(xiàn)性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse SPON1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 102
    Range % 97-106
    1:2 Average % 104
    Range % 99-108
    1:4 Average % 86
    Range % 81-92
    1:8 Average % 94
    Range % 89-98
  • 回收率：
    The recovery of mouse SPON1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 102 97-106
    EDTA plasma (n=4) 87 83-92
  • 標(biāo)準(zhǔn)曲線(xiàn)：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    10 2.249 2.342 2.296 2.142
    5 1.472 1.587 1.530 1.376
    2.5 0.925 0.964 0.945 0.791
    1.25 0.591 0.607 0.599 0.445
    0.625 0.392 0.386 0.389 0.235
    0.312 0.301 0.311 0.306 0.152
    0.156 0.218 0.222 0.220 0.066
    0 0.153 0.154 0.154 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Cell adhesion protein that promotes the attachment of spinal cord and sensory neuron cells and the outgrowth of neurites in vitro. May contribute to the growth and guidance of axons in both the spinal cord and the PNS.
  • 基因功能參考文獻(xiàn)：
    1. We conclude that upon ligand binding, TLR4 activates PI3K/Akt signaling to induce F-spondin expression, subsequently control CREB-mediated IL-6 production to promote VSMC migration. These findings provide vital insights into the essential role of F-spondin in VSMC function and will be valuable for developing new therapeutic strategies against atherosclerosis. PMID: 27091427
    2. These findings demonstrated novel roles for F-spondin and IL-1Ra in type B CpG oligodeoxynucleotides-mediated cell proliferation and migration of macrophages. PMID: 26042735
    3. Spon1 deletion leads to a local and systemic reduction of TGF-beta levels resulting in increased BMP signaling and increased bone deposition in adult mice. PMID: 24875054
    4. F-spondin is expressed in embryonic cartilage, where it has the capacity to enhance chondrocyte terminal differentiation and mineralization. PMID: 20839318
  • 亞細(xì)胞定位：
    Secreted, extracellular space, extracellular matrix.
  • 數(shù)據(jù)庫(kù)鏈接：