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  4. Mouse Tetranectin(CLEC3B) ELISA kit

Mouse Tetranectin(CLEC3B) ELISA kit

  • 中文名稱：
    小鼠四連接素(CLEC3B)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL005531MO
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠四連接素(CLEC3B)酶聯(lián)免疫試劑盒（CSB-EL005531MO）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的CLEC3B含量。CLEC3B，也稱為四連蛋白，是一種分泌的同源三聚體蛋白，主要存在于血漿中。它能夠與纖溶酶原和kringle 4結(jié)合，可能參與胞吐作用的分子包裝。CLEC3B的表達(dá)與癌癥進(jìn)展和不良預(yù)后相關(guān)，可能通過(guò)調(diào)節(jié)免疫細(xì)胞浸潤(rùn)和抑制細(xì)胞增殖來(lái)影響腫瘤發(fā)展。試劑盒檢測(cè)范圍為1.56 ng/mL-100 ng/mL，該產(chǎn)品廣泛應(yīng)用于腫瘤生物學(xué)、細(xì)胞信號(hào)轉(zhuǎn)導(dǎo)等領(lǐng)域的實(shí)驗(yàn)研究，特別適用于基因敲除/過(guò)表達(dá)模型的蛋白表達(dá)驗(yàn)證、疾病動(dòng)物模型的分子標(biāo)志物動(dòng)態(tài)監(jiān)測(cè)等科研場(chǎng)景。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
  • 別名：
    Clec3b ELISA Kit; Tna ELISA Kit; Tetranectin ELISA Kit; TN ELISA Kit; C-type lectin domain family 3 member B ELISA Kit; Plasminogen kringle 4-binding protein ELISA Kit
  • 縮寫：
    CLEC3B
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    1.56 ng/mL-100 ng/mL
  • 靈敏度：
    0.39 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Others
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse CLEC3B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:100 Average % 104    
    Range % 100-112    
    1:200 Average % 90    
    Range % 86-94    
    1:400 Average % 86    
    Range % 82-90    
    1:800 Average % 93    
    Range % 89-98    
  • 回收率：
    The recovery of mouse CLEC3B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 95 90-100    
    EDTA plasma (n=4) 88 84-92    
                   
                   
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    ng/ml OD1 OD2 Average Corrected    
    100 2.221 2.380 2.301 2.162    
    50 1.975 2.014 1.995 1.856    
    25 1.616 1.528 1.572 1.433    
    12.5 1.004 0.979 0.992 0.853    
    6.25 0.634 0.652 0.643 0.504    
    3.12 0.392 0.407 0.400 0.261    
    1.56 0.250 0.243 0.247 0.108    
    0 0.142 0.135 0.139 　    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Tetranectin binds to plasminogen and to isolated kringle 4. May be involved in the packaging of molecules destined for exocytosis.
  • 亞細(xì)胞定位：
    Secreted.
  • 組織特異性：
    Highest expression in lung and skeletal muscle.
  • 數(shù)據(jù)庫(kù)鏈接：